A serological assay to detect SARS-CoV-2 antibodies in at-home collected finger-prick dried blood spots

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Accurate surveillance of coronavirus disease 2019 (COVID-19) incidence requires large-scale testing of the population. Current testing methods require in-person collection of biospecimens by a healthcare worker, limiting access of individuals who do not have access to testing facilities while placin...

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Autores principales: Karp, Donna Grace, Danh, Kenneth, Seftel, David, Robinson, Peter, Tsai, Cheng-ting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7273297/
https://www.ncbi.nlm.nih.gov/pubmed/32511529
http://dx.doi.org/10.1101/2020.05.29.20116004
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author Karp, Donna Grace
Danh, Kenneth
Seftel, David
Robinson, Peter
Tsai, Cheng-ting
author_facet Karp, Donna Grace
Danh, Kenneth
Seftel, David
Robinson, Peter
Tsai, Cheng-ting
author_sort Karp, Donna Grace
collection PubMed
description Accurate surveillance of coronavirus disease 2019 (COVID-19) incidence requires large-scale testing of the population. Current testing methods require in-person collection of biospecimens by a healthcare worker, limiting access of individuals who do not have access to testing facilities while placing both the patient and healthcare worker at risk of exposure to infection. We report the development and validation of a at-home finger-prick dried blood spot collection kit and an analysis method. We demonstrated 100% sensitivity and specificity using at-home collected specimens across the US. Such methods may facilitate the conduct of unbiased serosurveys within hard to reach populations and help reduce the sample collection burden of serological testing on both health care systems and individuals alike.
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spelling pubmed-72732972020-06-07 A serological assay to detect SARS-CoV-2 antibodies in at-home collected finger-prick dried blood spots Karp, Donna Grace Danh, Kenneth Seftel, David Robinson, Peter Tsai, Cheng-ting medRxiv Article Accurate surveillance of coronavirus disease 2019 (COVID-19) incidence requires large-scale testing of the population. Current testing methods require in-person collection of biospecimens by a healthcare worker, limiting access of individuals who do not have access to testing facilities while placing both the patient and healthcare worker at risk of exposure to infection. We report the development and validation of a at-home finger-prick dried blood spot collection kit and an analysis method. We demonstrated 100% sensitivity and specificity using at-home collected specimens across the US. Such methods may facilitate the conduct of unbiased serosurveys within hard to reach populations and help reduce the sample collection burden of serological testing on both health care systems and individuals alike. Cold Spring Harbor Laboratory 2020-06-03 /pmc/articles/PMC7273297/ /pubmed/32511529 http://dx.doi.org/10.1101/2020.05.29.20116004 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Article
Karp, Donna Grace
Danh, Kenneth
Seftel, David
Robinson, Peter
Tsai, Cheng-ting
A serological assay to detect SARS-CoV-2 antibodies in at-home collected finger-prick dried blood spots
title A serological assay to detect SARS-CoV-2 antibodies in at-home collected finger-prick dried blood spots
title_full A serological assay to detect SARS-CoV-2 antibodies in at-home collected finger-prick dried blood spots
title_fullStr A serological assay to detect SARS-CoV-2 antibodies in at-home collected finger-prick dried blood spots
title_full_unstemmed A serological assay to detect SARS-CoV-2 antibodies in at-home collected finger-prick dried blood spots
title_short A serological assay to detect SARS-CoV-2 antibodies in at-home collected finger-prick dried blood spots
title_sort serological assay to detect sars-cov-2 antibodies in at-home collected finger-prick dried blood spots
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7273297/
https://www.ncbi.nlm.nih.gov/pubmed/32511529
http://dx.doi.org/10.1101/2020.05.29.20116004
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