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Iron-Chelating Agent Can Maintain Bone Homeostasis Disrupted by Iron Overload by Upregulating Wnt/Beta-Catenin Signaling

BACKGROUND: The incidence of osteoporotic fractures is increasing. In this study, we explored the activities of Wnt/β-catenin signaling in bone tissues with iron accumulation. METHODS: We established rat bipedal walking models (RBWM), and a portion of our RBWM rats were intraperitoneally injected wi...

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Autores principales: Xu, Wei, Yu, Ronghua, Zhu, Xiaodong, Li, Zhikun, Jia, Jianjun, Li, Dachuan, Chen, Yu, Zhang, Xiangyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7273370/
https://www.ncbi.nlm.nih.gov/pubmed/32596380
http://dx.doi.org/10.1155/2020/8256261
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author Xu, Wei
Yu, Ronghua
Zhu, Xiaodong
Li, Zhikun
Jia, Jianjun
Li, Dachuan
Chen, Yu
Zhang, Xiangyang
author_facet Xu, Wei
Yu, Ronghua
Zhu, Xiaodong
Li, Zhikun
Jia, Jianjun
Li, Dachuan
Chen, Yu
Zhang, Xiangyang
author_sort Xu, Wei
collection PubMed
description BACKGROUND: The incidence of osteoporotic fractures is increasing. In this study, we explored the activities of Wnt/β-catenin signaling in bone tissues with iron accumulation. METHODS: We established rat bipedal walking models (RBWM), and a portion of our RBWM rats were intraperitoneally injected with ferric ammonium citrate, normal saline, and deferoxamine. Bone mineral density was measured with a small animal in vivo imaging system. The protein levels of ferritin, TRAP-5B, RANKL, and OPG in serum were measured by the enzyme-linked immunosorbent assay (ELISA). Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot were used to quantify the RNA and protein expression levels of certain regulators involved in Wnt/β-catenin signaling in bone tissues. RESULTS: In the present study, we established a rat bipedal walking model containing 32 bipedal rats, which were randomly classified into four groups, termed as NS, FAC, FAC+NS, and FAC+DFO. Those three experimental groups with FAC injection had significantly lower bone mineral density (BMD) than the control group NS (P < 0.05). The disruption of bone homeostasis and downregulation of Wnt/β-catenin signaling were also observed in the three groups with FAC injection. Moreover, after the injection of deferoxamine, those aberrations in samples with FAC injection seemed repaired as test results returning or getting close to normal ranges. CONCLUSION: The osteoporosis could be caused by iron overload, which reduced the bone mineral density by disrupting the homeostasis of bone formation and absorption and attenuating the Wnt/β-catenin signaling in bone tissues. The deferoxamine had the potential to improve the bone health by reducing the accumulation of iron and increasing the bone mass, which might be a promising therapeutic solution for osteoporosis.
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spelling pubmed-72733702020-06-27 Iron-Chelating Agent Can Maintain Bone Homeostasis Disrupted by Iron Overload by Upregulating Wnt/Beta-Catenin Signaling Xu, Wei Yu, Ronghua Zhu, Xiaodong Li, Zhikun Jia, Jianjun Li, Dachuan Chen, Yu Zhang, Xiangyang Biomed Res Int Research Article BACKGROUND: The incidence of osteoporotic fractures is increasing. In this study, we explored the activities of Wnt/β-catenin signaling in bone tissues with iron accumulation. METHODS: We established rat bipedal walking models (RBWM), and a portion of our RBWM rats were intraperitoneally injected with ferric ammonium citrate, normal saline, and deferoxamine. Bone mineral density was measured with a small animal in vivo imaging system. The protein levels of ferritin, TRAP-5B, RANKL, and OPG in serum were measured by the enzyme-linked immunosorbent assay (ELISA). Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot were used to quantify the RNA and protein expression levels of certain regulators involved in Wnt/β-catenin signaling in bone tissues. RESULTS: In the present study, we established a rat bipedal walking model containing 32 bipedal rats, which were randomly classified into four groups, termed as NS, FAC, FAC+NS, and FAC+DFO. Those three experimental groups with FAC injection had significantly lower bone mineral density (BMD) than the control group NS (P < 0.05). The disruption of bone homeostasis and downregulation of Wnt/β-catenin signaling were also observed in the three groups with FAC injection. Moreover, after the injection of deferoxamine, those aberrations in samples with FAC injection seemed repaired as test results returning or getting close to normal ranges. CONCLUSION: The osteoporosis could be caused by iron overload, which reduced the bone mineral density by disrupting the homeostasis of bone formation and absorption and attenuating the Wnt/β-catenin signaling in bone tissues. The deferoxamine had the potential to improve the bone health by reducing the accumulation of iron and increasing the bone mass, which might be a promising therapeutic solution for osteoporosis. Hindawi 2020-05-24 /pmc/articles/PMC7273370/ /pubmed/32596380 http://dx.doi.org/10.1155/2020/8256261 Text en Copyright © 2020 Wei Xu et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Xu, Wei
Yu, Ronghua
Zhu, Xiaodong
Li, Zhikun
Jia, Jianjun
Li, Dachuan
Chen, Yu
Zhang, Xiangyang
Iron-Chelating Agent Can Maintain Bone Homeostasis Disrupted by Iron Overload by Upregulating Wnt/Beta-Catenin Signaling
title Iron-Chelating Agent Can Maintain Bone Homeostasis Disrupted by Iron Overload by Upregulating Wnt/Beta-Catenin Signaling
title_full Iron-Chelating Agent Can Maintain Bone Homeostasis Disrupted by Iron Overload by Upregulating Wnt/Beta-Catenin Signaling
title_fullStr Iron-Chelating Agent Can Maintain Bone Homeostasis Disrupted by Iron Overload by Upregulating Wnt/Beta-Catenin Signaling
title_full_unstemmed Iron-Chelating Agent Can Maintain Bone Homeostasis Disrupted by Iron Overload by Upregulating Wnt/Beta-Catenin Signaling
title_short Iron-Chelating Agent Can Maintain Bone Homeostasis Disrupted by Iron Overload by Upregulating Wnt/Beta-Catenin Signaling
title_sort iron-chelating agent can maintain bone homeostasis disrupted by iron overload by upregulating wnt/beta-catenin signaling
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7273370/
https://www.ncbi.nlm.nih.gov/pubmed/32596380
http://dx.doi.org/10.1155/2020/8256261
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