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In Vitro Biocompatibility Evaluation of Nine Dermal Fillers on L929 Cell Line
OBJECTIVE: Biomaterial research for soft tissue augmentation is an increasing topic in aesthetic medicine. Hyaluronic acid (HA) fillers are widely used for their low invasiveness and easy application to correct aesthetic defects or traumatic injuries. Some complications as acute or chronic inflammat...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7273492/ https://www.ncbi.nlm.nih.gov/pubmed/32596390 http://dx.doi.org/10.1155/2020/8676343 |
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author | Cannella, Vincenza Altomare, Roberta Leonardi, Vincenza Russotto, Laura Di Bella, Santina Mira, Francesco Guercio, Annalisa |
author_facet | Cannella, Vincenza Altomare, Roberta Leonardi, Vincenza Russotto, Laura Di Bella, Santina Mira, Francesco Guercio, Annalisa |
author_sort | Cannella, Vincenza |
collection | PubMed |
description | OBJECTIVE: Biomaterial research for soft tissue augmentation is an increasing topic in aesthetic medicine. Hyaluronic acid (HA) fillers are widely used for their low invasiveness and easy application to correct aesthetic defects or traumatic injuries. Some complications as acute or chronic inflammation can occur in patients following the injection. Biocompatibility assays are required for medical devices intended for human use, in order to prevent damages or injuries in the host. In this study, nine HA fillers were tested in order to evaluate their cytotoxicity and their effects on L929 cell line, according to the UNI EN ISO 10993 regulation. METHODS: Extracts were prepared from nine HA fillers, and MTS viability assay was performed after 24 h, 48 h, and 72 h of exposure of cells to extracts. Cells cultured with HA filler extracts were monitored for up to 72 h, counted, and stained with haematoxylin/eosin in order to evaluate the cell proliferation rate and morphology. RESULTS: None of the filler tested showed a cytotoxic effect. Two samples showed a higher vitality percentage and higher cell number while two samples showed a lower vitality percentage and lower cell number at 72 h. CONCLUSION: Data obtained suggest that although examined fillers are not cytotoxic, they show different effects on the in vitro cell proliferation rate. In vitro studies of medical devices could lead to important implications since these could aid to predict effects about their in vivo application. These easy and rapid assays could be useful to test new materials intended for human use avoiding animal tests. |
format | Online Article Text |
id | pubmed-7273492 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-72734922020-06-27 In Vitro Biocompatibility Evaluation of Nine Dermal Fillers on L929 Cell Line Cannella, Vincenza Altomare, Roberta Leonardi, Vincenza Russotto, Laura Di Bella, Santina Mira, Francesco Guercio, Annalisa Biomed Res Int Research Article OBJECTIVE: Biomaterial research for soft tissue augmentation is an increasing topic in aesthetic medicine. Hyaluronic acid (HA) fillers are widely used for their low invasiveness and easy application to correct aesthetic defects or traumatic injuries. Some complications as acute or chronic inflammation can occur in patients following the injection. Biocompatibility assays are required for medical devices intended for human use, in order to prevent damages or injuries in the host. In this study, nine HA fillers were tested in order to evaluate their cytotoxicity and their effects on L929 cell line, according to the UNI EN ISO 10993 regulation. METHODS: Extracts were prepared from nine HA fillers, and MTS viability assay was performed after 24 h, 48 h, and 72 h of exposure of cells to extracts. Cells cultured with HA filler extracts were monitored for up to 72 h, counted, and stained with haematoxylin/eosin in order to evaluate the cell proliferation rate and morphology. RESULTS: None of the filler tested showed a cytotoxic effect. Two samples showed a higher vitality percentage and higher cell number while two samples showed a lower vitality percentage and lower cell number at 72 h. CONCLUSION: Data obtained suggest that although examined fillers are not cytotoxic, they show different effects on the in vitro cell proliferation rate. In vitro studies of medical devices could lead to important implications since these could aid to predict effects about their in vivo application. These easy and rapid assays could be useful to test new materials intended for human use avoiding animal tests. Hindawi 2020-05-21 /pmc/articles/PMC7273492/ /pubmed/32596390 http://dx.doi.org/10.1155/2020/8676343 Text en Copyright © 2020 Vincenza Cannella et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Cannella, Vincenza Altomare, Roberta Leonardi, Vincenza Russotto, Laura Di Bella, Santina Mira, Francesco Guercio, Annalisa In Vitro Biocompatibility Evaluation of Nine Dermal Fillers on L929 Cell Line |
title | In Vitro Biocompatibility Evaluation of Nine Dermal Fillers on L929 Cell Line |
title_full | In Vitro Biocompatibility Evaluation of Nine Dermal Fillers on L929 Cell Line |
title_fullStr | In Vitro Biocompatibility Evaluation of Nine Dermal Fillers on L929 Cell Line |
title_full_unstemmed | In Vitro Biocompatibility Evaluation of Nine Dermal Fillers on L929 Cell Line |
title_short | In Vitro Biocompatibility Evaluation of Nine Dermal Fillers on L929 Cell Line |
title_sort | in vitro biocompatibility evaluation of nine dermal fillers on l929 cell line |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7273492/ https://www.ncbi.nlm.nih.gov/pubmed/32596390 http://dx.doi.org/10.1155/2020/8676343 |
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