Cargando…
Morphological and molecular identification of Eimeria spp. in breeding chicken farms of Japan
There have been no reports of the prevalence of Eimeria spp. in poultry breeding farms in Japan unlike those of broiler farms. From 2017 to 2018, we examined the prevalence of Eimeria spp. on breeding farms in Japan by oocyst morphology and PCR analyses. A total of 143 samples was collected from 37...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japanese Society of Veterinary Science
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7273590/ https://www.ncbi.nlm.nih.gov/pubmed/32238624 http://dx.doi.org/10.1292/jvms.19-0661 |
_version_ | 1783542436703764480 |
---|---|
author | MATSUBAYASHI, Makoto SHIBAHARA, Tomoyuki MATSUO, Tomohide HATABU, Toshimitsu YAMAGISHI, Junya SASAI, Kazumi ISOBE, Takashi |
author_facet | MATSUBAYASHI, Makoto SHIBAHARA, Tomoyuki MATSUO, Tomohide HATABU, Toshimitsu YAMAGISHI, Junya SASAI, Kazumi ISOBE, Takashi |
author_sort | MATSUBAYASHI, Makoto |
collection | PubMed |
description | There have been no reports of the prevalence of Eimeria spp. in poultry breeding farms in Japan unlike those of broiler farms. From 2017 to 2018, we examined the prevalence of Eimeria spp. on breeding farms in Japan by oocyst morphology and PCR analyses. A total of 143 samples was collected from 37 breeding farms in 21 prefectures of Japan. We detected oocysts of seven species at 34 of 37 breeding farms by PCR, and we identified E. brunetti at 51.5% of farms found to be positive for Eimeria. The differences in the identification of Eimeria spp. between the morphology and PCR assay methods of oocysts were pronounced for E. maxima and E. necatrix. We confirmed that molecular tools were more suitable for accurately estimating prevalence of Eimeria spp., and these findings suggest that E. brunetti could be widespread in Japan. |
format | Online Article Text |
id | pubmed-7273590 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | The Japanese Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-72735902020-06-10 Morphological and molecular identification of Eimeria spp. in breeding chicken farms of Japan MATSUBAYASHI, Makoto SHIBAHARA, Tomoyuki MATSUO, Tomohide HATABU, Toshimitsu YAMAGISHI, Junya SASAI, Kazumi ISOBE, Takashi J Vet Med Sci Avian Pathology There have been no reports of the prevalence of Eimeria spp. in poultry breeding farms in Japan unlike those of broiler farms. From 2017 to 2018, we examined the prevalence of Eimeria spp. on breeding farms in Japan by oocyst morphology and PCR analyses. A total of 143 samples was collected from 37 breeding farms in 21 prefectures of Japan. We detected oocysts of seven species at 34 of 37 breeding farms by PCR, and we identified E. brunetti at 51.5% of farms found to be positive for Eimeria. The differences in the identification of Eimeria spp. between the morphology and PCR assay methods of oocysts were pronounced for E. maxima and E. necatrix. We confirmed that molecular tools were more suitable for accurately estimating prevalence of Eimeria spp., and these findings suggest that E. brunetti could be widespread in Japan. The Japanese Society of Veterinary Science 2020-04-01 2020-05 /pmc/articles/PMC7273590/ /pubmed/32238624 http://dx.doi.org/10.1292/jvms.19-0661 Text en ©2020 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Avian Pathology MATSUBAYASHI, Makoto SHIBAHARA, Tomoyuki MATSUO, Tomohide HATABU, Toshimitsu YAMAGISHI, Junya SASAI, Kazumi ISOBE, Takashi Morphological and molecular identification of Eimeria spp. in breeding chicken farms of Japan |
title | Morphological and molecular identification of Eimeria spp. in breeding chicken farms of Japan |
title_full | Morphological and molecular identification of Eimeria spp. in breeding chicken farms of Japan |
title_fullStr | Morphological and molecular identification of Eimeria spp. in breeding chicken farms of Japan |
title_full_unstemmed | Morphological and molecular identification of Eimeria spp. in breeding chicken farms of Japan |
title_short | Morphological and molecular identification of Eimeria spp. in breeding chicken farms of Japan |
title_sort | morphological and molecular identification of eimeria spp. in breeding chicken farms of japan |
topic | Avian Pathology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7273590/ https://www.ncbi.nlm.nih.gov/pubmed/32238624 http://dx.doi.org/10.1292/jvms.19-0661 |
work_keys_str_mv | AT matsubayashimakoto morphologicalandmolecularidentificationofeimeriasppinbreedingchickenfarmsofjapan AT shibaharatomoyuki morphologicalandmolecularidentificationofeimeriasppinbreedingchickenfarmsofjapan AT matsuotomohide morphologicalandmolecularidentificationofeimeriasppinbreedingchickenfarmsofjapan AT hatabutoshimitsu morphologicalandmolecularidentificationofeimeriasppinbreedingchickenfarmsofjapan AT yamagishijunya morphologicalandmolecularidentificationofeimeriasppinbreedingchickenfarmsofjapan AT sasaikazumi morphologicalandmolecularidentificationofeimeriasppinbreedingchickenfarmsofjapan AT isobetakashi morphologicalandmolecularidentificationofeimeriasppinbreedingchickenfarmsofjapan |