Canine mesenchymal stromal cell-conditioned medium promotes survival and neurite outgrowth of neural stem cells

We examined the paracrine action of canine mesenchymal stromal cells (MSCs) derived from bone marrow on the survival and differentiation of neural stem cells (NSCs) in vitro. MSCs were collected from the proximal end of the diaphysis of femur of healthy beagle dogs. The 70–80% confluent MSCs were re...

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Detalles Bibliográficos
Autores principales: NAKAMURA, Michi, NISHIDA, Hidetaka, YOSHIZAKI, Karin, AKIYOSHI, Hideo, HATOYA, Shingo, SUGIURA, Kikuya, INABA, Toshio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2020
Materias:
Surgery
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7273601/
https://www.ncbi.nlm.nih.gov/pubmed/32249241
http://dx.doi.org/10.1292/jvms.19-0141
Descripción
Sumario:We examined the paracrine action of canine mesenchymal stromal cells (MSCs) derived from bone marrow on the survival and differentiation of neural stem cells (NSCs) in vitro. MSCs were collected from the proximal end of the diaphysis of femur of healthy beagle dogs. The 70–80% confluent MSCs were re-fed with serum-free DMEM. The MSCs were incubated for 48 hr and the supernatant was collected as the conditioned medium (MSC-CM). The survival rate of NSCs in MSC-CM was significantly greater than in the medium without MSC-CM. The percentage of differentiated neurons and neurite length in MSC-CM was also significantly higher than in the medium without MSC-CM. These results suggested that canine MSC-CM promotes stem cell survival and neural differentiation of NSCs.

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