Canine mesenchymal stromal cell-conditioned medium promotes survival and neurite outgrowth of neural stem cells

We examined the paracrine action of canine mesenchymal stromal cells (MSCs) derived from bone marrow on the survival and differentiation of neural stem cells (NSCs) in vitro. MSCs were collected from the proximal end of the diaphysis of femur of healthy beagle dogs. The 70–80% confluent MSCs were re...

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Autores principales: NAKAMURA, Michi, NISHIDA, Hidetaka, YOSHIZAKI, Karin, AKIYOSHI, Hideo, HATOYA, Shingo, SUGIURA, Kikuya, INABA, Toshio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2020
Materias:
Surgery
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7273601/
https://www.ncbi.nlm.nih.gov/pubmed/32249241
http://dx.doi.org/10.1292/jvms.19-0141
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author NAKAMURA, Michi
NISHIDA, Hidetaka
YOSHIZAKI, Karin
AKIYOSHI, Hideo
HATOYA, Shingo
SUGIURA, Kikuya
INABA, Toshio
author_facet NAKAMURA, Michi
NISHIDA, Hidetaka
YOSHIZAKI, Karin
AKIYOSHI, Hideo
HATOYA, Shingo
SUGIURA, Kikuya
INABA, Toshio
author_sort NAKAMURA, Michi
collection PubMed
description We examined the paracrine action of canine mesenchymal stromal cells (MSCs) derived from bone marrow on the survival and differentiation of neural stem cells (NSCs) in vitro. MSCs were collected from the proximal end of the diaphysis of femur of healthy beagle dogs. The 70–80% confluent MSCs were re-fed with serum-free DMEM. The MSCs were incubated for 48 hr and the supernatant was collected as the conditioned medium (MSC-CM). The survival rate of NSCs in MSC-CM was significantly greater than in the medium without MSC-CM. The percentage of differentiated neurons and neurite length in MSC-CM was also significantly higher than in the medium without MSC-CM. These results suggested that canine MSC-CM promotes stem cell survival and neural differentiation of NSCs.
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spelling pubmed-72736012020-06-10 Canine mesenchymal stromal cell-conditioned medium promotes survival and neurite outgrowth of neural stem cells NAKAMURA, Michi NISHIDA, Hidetaka YOSHIZAKI, Karin AKIYOSHI, Hideo HATOYA, Shingo SUGIURA, Kikuya INABA, Toshio J Vet Med Sci Surgery We examined the paracrine action of canine mesenchymal stromal cells (MSCs) derived from bone marrow on the survival and differentiation of neural stem cells (NSCs) in vitro. MSCs were collected from the proximal end of the diaphysis of femur of healthy beagle dogs. The 70–80% confluent MSCs were re-fed with serum-free DMEM. The MSCs were incubated for 48 hr and the supernatant was collected as the conditioned medium (MSC-CM). The survival rate of NSCs in MSC-CM was significantly greater than in the medium without MSC-CM. The percentage of differentiated neurons and neurite length in MSC-CM was also significantly higher than in the medium without MSC-CM. These results suggested that canine MSC-CM promotes stem cell survival and neural differentiation of NSCs. The Japanese Society of Veterinary Science 2020-04-06 2020-05 /pmc/articles/PMC7273601/ /pubmed/32249241 http://dx.doi.org/10.1292/jvms.19-0141 Text en ©2020 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Surgery
NAKAMURA, Michi
NISHIDA, Hidetaka
YOSHIZAKI, Karin
AKIYOSHI, Hideo
HATOYA, Shingo
SUGIURA, Kikuya
INABA, Toshio
Canine mesenchymal stromal cell-conditioned medium promotes survival and neurite outgrowth of neural stem cells
title Canine mesenchymal stromal cell-conditioned medium promotes survival and neurite outgrowth of neural stem cells
title_full Canine mesenchymal stromal cell-conditioned medium promotes survival and neurite outgrowth of neural stem cells
title_fullStr Canine mesenchymal stromal cell-conditioned medium promotes survival and neurite outgrowth of neural stem cells
title_full_unstemmed Canine mesenchymal stromal cell-conditioned medium promotes survival and neurite outgrowth of neural stem cells
title_short Canine mesenchymal stromal cell-conditioned medium promotes survival and neurite outgrowth of neural stem cells
title_sort canine mesenchymal stromal cell-conditioned medium promotes survival and neurite outgrowth of neural stem cells
topic Surgery
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7273601/
https://www.ncbi.nlm.nih.gov/pubmed/32249241
http://dx.doi.org/10.1292/jvms.19-0141
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