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Cotinine inhibits TLR4/NF-κB signaling pathway and improves deep vein thrombosis in rats
Background: The present study was designed to explore the regulatory mechanisms and influences of cotinine on deep vein thrombosis (DVT) in rats via the toll-like receptor 4/nuclear factor κ binding (TLR-4/NF-κB) pathway. Methods: In this experimental study, 30 SD rats were randomly assigned to cont...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7273908/ https://www.ncbi.nlm.nih.gov/pubmed/32441737 http://dx.doi.org/10.1042/BSR20201293 |
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author | Cheng, Zhiyuan Jia, Wei Tian, Xuan Jiang, Peng Zhang, Yunxin Li, Jinyong Tian, Chenyang Liu, Jianlong |
author_facet | Cheng, Zhiyuan Jia, Wei Tian, Xuan Jiang, Peng Zhang, Yunxin Li, Jinyong Tian, Chenyang Liu, Jianlong |
author_sort | Cheng, Zhiyuan |
collection | PubMed |
description | Background: The present study was designed to explore the regulatory mechanisms and influences of cotinine on deep vein thrombosis (DVT) in rats via the toll-like receptor 4/nuclear factor κ binding (TLR-4/NF-κB) pathway. Methods: In this experimental study, 30 SD rats were randomly assigned to control group, sham operation group, model group, cotinine (10 μg/kg) group, and model + cotinine (10 μg/kg) group. The thromboxane B2 (TXB2), 6-keto-PGF(1α), plasminogen activator inhibitor (PAI), tissue plasminogen activator (t-PA), TLR4, NF-κB, and p65 mRNA and protein expression and tissue changes were analyzed by ELISA, Hematoxylin–Eosin (HE) staining, RT-PCR, and Western blot. Results: There was no significant difference between the control and sham operation groups (P>0.05). The model and cotinine groups showed significantly higher mRNA and protein levels of TXB2, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), PAI, TLR-4, and NF-κB, and significantly lower levels of 6-keto-PGF(1α) and t-PA than the control and sham operation groups (P<0.05), and the model + cotinine group showed significantly higher mRNA and protein levels of TXB2, IL-6 and TNF-α, PAI, TLR-4, and NF-κB and significantly lower levels of 6-keto-PGF(1α) and t-PA than the model group (P<0.05). Conclusion: Cotinine can aggravate thrombus and inflammation in rats with DVT, and the mechanism may be associated with the activation of the TLR-4/NF-κB inflammatory signaling pathway. |
format | Online Article Text |
id | pubmed-7273908 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-72739082020-06-16 Cotinine inhibits TLR4/NF-κB signaling pathway and improves deep vein thrombosis in rats Cheng, Zhiyuan Jia, Wei Tian, Xuan Jiang, Peng Zhang, Yunxin Li, Jinyong Tian, Chenyang Liu, Jianlong Biosci Rep Signaling Background: The present study was designed to explore the regulatory mechanisms and influences of cotinine on deep vein thrombosis (DVT) in rats via the toll-like receptor 4/nuclear factor κ binding (TLR-4/NF-κB) pathway. Methods: In this experimental study, 30 SD rats were randomly assigned to control group, sham operation group, model group, cotinine (10 μg/kg) group, and model + cotinine (10 μg/kg) group. The thromboxane B2 (TXB2), 6-keto-PGF(1α), plasminogen activator inhibitor (PAI), tissue plasminogen activator (t-PA), TLR4, NF-κB, and p65 mRNA and protein expression and tissue changes were analyzed by ELISA, Hematoxylin–Eosin (HE) staining, RT-PCR, and Western blot. Results: There was no significant difference between the control and sham operation groups (P>0.05). The model and cotinine groups showed significantly higher mRNA and protein levels of TXB2, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), PAI, TLR-4, and NF-κB, and significantly lower levels of 6-keto-PGF(1α) and t-PA than the control and sham operation groups (P<0.05), and the model + cotinine group showed significantly higher mRNA and protein levels of TXB2, IL-6 and TNF-α, PAI, TLR-4, and NF-κB and significantly lower levels of 6-keto-PGF(1α) and t-PA than the model group (P<0.05). Conclusion: Cotinine can aggravate thrombus and inflammation in rats with DVT, and the mechanism may be associated with the activation of the TLR-4/NF-κB inflammatory signaling pathway. Portland Press Ltd. 2020-06-04 /pmc/articles/PMC7273908/ /pubmed/32441737 http://dx.doi.org/10.1042/BSR20201293 Text en © 2020 The Author(s). https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY). |
spellingShingle | Signaling Cheng, Zhiyuan Jia, Wei Tian, Xuan Jiang, Peng Zhang, Yunxin Li, Jinyong Tian, Chenyang Liu, Jianlong Cotinine inhibits TLR4/NF-κB signaling pathway and improves deep vein thrombosis in rats |
title | Cotinine inhibits TLR4/NF-κB signaling pathway and improves deep vein thrombosis in rats |
title_full | Cotinine inhibits TLR4/NF-κB signaling pathway and improves deep vein thrombosis in rats |
title_fullStr | Cotinine inhibits TLR4/NF-κB signaling pathway and improves deep vein thrombosis in rats |
title_full_unstemmed | Cotinine inhibits TLR4/NF-κB signaling pathway and improves deep vein thrombosis in rats |
title_short | Cotinine inhibits TLR4/NF-κB signaling pathway and improves deep vein thrombosis in rats |
title_sort | cotinine inhibits tlr4/nf-κb signaling pathway and improves deep vein thrombosis in rats |
topic | Signaling |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7273908/ https://www.ncbi.nlm.nih.gov/pubmed/32441737 http://dx.doi.org/10.1042/BSR20201293 |
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