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Cotinine inhibits TLR4/NF-κB signaling pathway and improves deep vein thrombosis in rats

Background: The present study was designed to explore the regulatory mechanisms and influences of cotinine on deep vein thrombosis (DVT) in rats via the toll-like receptor 4/nuclear factor κ binding (TLR-4/NF-κB) pathway. Methods: In this experimental study, 30 SD rats were randomly assigned to cont...

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Autores principales: Cheng, Zhiyuan, Jia, Wei, Tian, Xuan, Jiang, Peng, Zhang, Yunxin, Li, Jinyong, Tian, Chenyang, Liu, Jianlong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7273908/
https://www.ncbi.nlm.nih.gov/pubmed/32441737
http://dx.doi.org/10.1042/BSR20201293
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author Cheng, Zhiyuan
Jia, Wei
Tian, Xuan
Jiang, Peng
Zhang, Yunxin
Li, Jinyong
Tian, Chenyang
Liu, Jianlong
author_facet Cheng, Zhiyuan
Jia, Wei
Tian, Xuan
Jiang, Peng
Zhang, Yunxin
Li, Jinyong
Tian, Chenyang
Liu, Jianlong
author_sort Cheng, Zhiyuan
collection PubMed
description Background: The present study was designed to explore the regulatory mechanisms and influences of cotinine on deep vein thrombosis (DVT) in rats via the toll-like receptor 4/nuclear factor κ binding (TLR-4/NF-κB) pathway. Methods: In this experimental study, 30 SD rats were randomly assigned to control group, sham operation group, model group, cotinine (10 μg/kg) group, and model + cotinine (10 μg/kg) group. The thromboxane B2 (TXB2), 6-keto-PGF(1α), plasminogen activator inhibitor (PAI), tissue plasminogen activator (t-PA), TLR4, NF-κB, and p65 mRNA and protein expression and tissue changes were analyzed by ELISA, Hematoxylin–Eosin (HE) staining, RT-PCR, and Western blot. Results: There was no significant difference between the control and sham operation groups (P>0.05). The model and cotinine groups showed significantly higher mRNA and protein levels of TXB2, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), PAI, TLR-4, and NF-κB, and significantly lower levels of 6-keto-PGF(1α) and t-PA than the control and sham operation groups (P<0.05), and the model + cotinine group showed significantly higher mRNA and protein levels of TXB2, IL-6 and TNF-α, PAI, TLR-4, and NF-κB and significantly lower levels of 6-keto-PGF(1α) and t-PA than the model group (P<0.05). Conclusion: Cotinine can aggravate thrombus and inflammation in rats with DVT, and the mechanism may be associated with the activation of the TLR-4/NF-κB inflammatory signaling pathway.
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spelling pubmed-72739082020-06-16 Cotinine inhibits TLR4/NF-κB signaling pathway and improves deep vein thrombosis in rats Cheng, Zhiyuan Jia, Wei Tian, Xuan Jiang, Peng Zhang, Yunxin Li, Jinyong Tian, Chenyang Liu, Jianlong Biosci Rep Signaling Background: The present study was designed to explore the regulatory mechanisms and influences of cotinine on deep vein thrombosis (DVT) in rats via the toll-like receptor 4/nuclear factor κ binding (TLR-4/NF-κB) pathway. Methods: In this experimental study, 30 SD rats were randomly assigned to control group, sham operation group, model group, cotinine (10 μg/kg) group, and model + cotinine (10 μg/kg) group. The thromboxane B2 (TXB2), 6-keto-PGF(1α), plasminogen activator inhibitor (PAI), tissue plasminogen activator (t-PA), TLR4, NF-κB, and p65 mRNA and protein expression and tissue changes were analyzed by ELISA, Hematoxylin–Eosin (HE) staining, RT-PCR, and Western blot. Results: There was no significant difference between the control and sham operation groups (P>0.05). The model and cotinine groups showed significantly higher mRNA and protein levels of TXB2, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), PAI, TLR-4, and NF-κB, and significantly lower levels of 6-keto-PGF(1α) and t-PA than the control and sham operation groups (P<0.05), and the model + cotinine group showed significantly higher mRNA and protein levels of TXB2, IL-6 and TNF-α, PAI, TLR-4, and NF-κB and significantly lower levels of 6-keto-PGF(1α) and t-PA than the model group (P<0.05). Conclusion: Cotinine can aggravate thrombus and inflammation in rats with DVT, and the mechanism may be associated with the activation of the TLR-4/NF-κB inflammatory signaling pathway. Portland Press Ltd. 2020-06-04 /pmc/articles/PMC7273908/ /pubmed/32441737 http://dx.doi.org/10.1042/BSR20201293 Text en © 2020 The Author(s). https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY).
spellingShingle Signaling
Cheng, Zhiyuan
Jia, Wei
Tian, Xuan
Jiang, Peng
Zhang, Yunxin
Li, Jinyong
Tian, Chenyang
Liu, Jianlong
Cotinine inhibits TLR4/NF-κB signaling pathway and improves deep vein thrombosis in rats
title Cotinine inhibits TLR4/NF-κB signaling pathway and improves deep vein thrombosis in rats
title_full Cotinine inhibits TLR4/NF-κB signaling pathway and improves deep vein thrombosis in rats
title_fullStr Cotinine inhibits TLR4/NF-κB signaling pathway and improves deep vein thrombosis in rats
title_full_unstemmed Cotinine inhibits TLR4/NF-κB signaling pathway and improves deep vein thrombosis in rats
title_short Cotinine inhibits TLR4/NF-κB signaling pathway and improves deep vein thrombosis in rats
title_sort cotinine inhibits tlr4/nf-κb signaling pathway and improves deep vein thrombosis in rats
topic Signaling
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7273908/
https://www.ncbi.nlm.nih.gov/pubmed/32441737
http://dx.doi.org/10.1042/BSR20201293
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