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MicroRNA-33a negatively regulates myoblast proliferation by targeting IGF1, follistatin and cyclin D1
MiR-33a is found as a regulator of cell proliferation in many cancer cells. However, it remains unknown if and how miR-33a plays a role in myoblast proliferation. To investigate the effect of miR-33a on myoblast proliferation, miR-33a mimic or inhibitor was co-administered with or without insulin-li...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7273915/ https://www.ncbi.nlm.nih.gov/pubmed/32436962 http://dx.doi.org/10.1042/BSR20191327 |
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author | Li, Xinxin Qiu, Jiamin Liu, Hehe Deng, Yan Hu, Shenqiang Hu, Jiwei Wang, Yushi Wang, Jiwen |
author_facet | Li, Xinxin Qiu, Jiamin Liu, Hehe Deng, Yan Hu, Shenqiang Hu, Jiwei Wang, Yushi Wang, Jiwen |
author_sort | Li, Xinxin |
collection | PubMed |
description | MiR-33a is found as a regulator of cell proliferation in many cancer cells. However, it remains unknown if and how miR-33a plays a role in myoblast proliferation. To investigate the effect of miR-33a on myoblast proliferation, miR-33a mimic or inhibitor was co-administered with or without insulin-like growth factor 1 (IGF1) to simulation myoblasts. Our study showed that up-regulation of miR-33a impaired myoblast proliferation, while down-regulation of miR-33a enhanced myoblast proliferation. Mechanistically, we examined that miR-33a can inhibit the transcription of IGF1, follistatin (FST) and cyclin D1 (CCND1) by targeting their 3′UTR region in both HEK293T cells and duck myoblasts. Moreover, up-regulation of miR-33a decreased and its down-regulation increased the mRNA expression of PI3K, Akt, mTOR and S6K. Importantly, the decreased PI3K, Akt, mTOR and S6K expression by miR-33a mimics was abrogated by co-administered with IGF1. Altogether, our results demonstrated that miR-33a may directly target IGF1, FST and CCND1 to inhibit myoblast proliferation via PI3K/Akt/mTOR signaling pathway. In conclusion, miR-33a is a potential negative regulator of myoblast proliferation and by modulating its expression could promote the early development of skeletal muscle. |
format | Online Article Text |
id | pubmed-7273915 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-72739152020-06-16 MicroRNA-33a negatively regulates myoblast proliferation by targeting IGF1, follistatin and cyclin D1 Li, Xinxin Qiu, Jiamin Liu, Hehe Deng, Yan Hu, Shenqiang Hu, Jiwei Wang, Yushi Wang, Jiwen Biosci Rep Cell Cycle, Growth & Proliferation MiR-33a is found as a regulator of cell proliferation in many cancer cells. However, it remains unknown if and how miR-33a plays a role in myoblast proliferation. To investigate the effect of miR-33a on myoblast proliferation, miR-33a mimic or inhibitor was co-administered with or without insulin-like growth factor 1 (IGF1) to simulation myoblasts. Our study showed that up-regulation of miR-33a impaired myoblast proliferation, while down-regulation of miR-33a enhanced myoblast proliferation. Mechanistically, we examined that miR-33a can inhibit the transcription of IGF1, follistatin (FST) and cyclin D1 (CCND1) by targeting their 3′UTR region in both HEK293T cells and duck myoblasts. Moreover, up-regulation of miR-33a decreased and its down-regulation increased the mRNA expression of PI3K, Akt, mTOR and S6K. Importantly, the decreased PI3K, Akt, mTOR and S6K expression by miR-33a mimics was abrogated by co-administered with IGF1. Altogether, our results demonstrated that miR-33a may directly target IGF1, FST and CCND1 to inhibit myoblast proliferation via PI3K/Akt/mTOR signaling pathway. In conclusion, miR-33a is a potential negative regulator of myoblast proliferation and by modulating its expression could promote the early development of skeletal muscle. Portland Press Ltd. 2020-06-04 /pmc/articles/PMC7273915/ /pubmed/32436962 http://dx.doi.org/10.1042/BSR20191327 Text en © 2020 The Author(s). https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY). |
spellingShingle | Cell Cycle, Growth & Proliferation Li, Xinxin Qiu, Jiamin Liu, Hehe Deng, Yan Hu, Shenqiang Hu, Jiwei Wang, Yushi Wang, Jiwen MicroRNA-33a negatively regulates myoblast proliferation by targeting IGF1, follistatin and cyclin D1 |
title | MicroRNA-33a negatively regulates myoblast proliferation by targeting IGF1, follistatin and cyclin D1 |
title_full | MicroRNA-33a negatively regulates myoblast proliferation by targeting IGF1, follistatin and cyclin D1 |
title_fullStr | MicroRNA-33a negatively regulates myoblast proliferation by targeting IGF1, follistatin and cyclin D1 |
title_full_unstemmed | MicroRNA-33a negatively regulates myoblast proliferation by targeting IGF1, follistatin and cyclin D1 |
title_short | MicroRNA-33a negatively regulates myoblast proliferation by targeting IGF1, follistatin and cyclin D1 |
title_sort | microrna-33a negatively regulates myoblast proliferation by targeting igf1, follistatin and cyclin d1 |
topic | Cell Cycle, Growth & Proliferation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7273915/ https://www.ncbi.nlm.nih.gov/pubmed/32436962 http://dx.doi.org/10.1042/BSR20191327 |
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