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MiR-486-3p inhibits the proliferation, migration and invasion of retinoblastoma cells by targeting ECM1
It has been reported that miR-486-3p expression is decreased in retinoblastoma (RB) tumor tissues, however, its function in RB has been less reported. The present study aimed to explore the regulatory effects of miR-486-3p on RB cells. The expression of miR-486-3p in RB tissues and cells was detecte...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7273916/ https://www.ncbi.nlm.nih.gov/pubmed/32401301 http://dx.doi.org/10.1042/BSR20200392 |
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author | Yang, Hongwei Huang, Yonggang He, Jian Chai, Guangrui Di, Yu Wang, Aiyuan Gui, Dongmei |
author_facet | Yang, Hongwei Huang, Yonggang He, Jian Chai, Guangrui Di, Yu Wang, Aiyuan Gui, Dongmei |
author_sort | Yang, Hongwei |
collection | PubMed |
description | It has been reported that miR-486-3p expression is decreased in retinoblastoma (RB) tumor tissues, however, its function in RB has been less reported. The present study aimed to explore the regulatory effects of miR-486-3p on RB cells. The expression of miR-486-3p in RB tissues and cells was detected by quantitative real-time polymerase chain reaction (qRT-PCR). Cell viability, proliferation, apoptosis, migration and invasion ability were determined by cell counting kit-8 (CCK-8) kit, clone formation assay, flow cytometry, scratch assay and transwell, respectively. Targetscan 7.2 and dual-luciferase reporter were used to verify target genes for miR-486-3p. The expressions of apoptosis-related proteins and ECM1 were detected by Western blot. The miR-486-3p expression was decreased in RB tissues and cells. In RB cells, overexpression of miR-486-3p inhibited cell proliferation, migration and invasion, while promoted apoptosis. Moreover, overexpression of miR-486-3p decreased Bcl-2 expression, while increased the expressions of Bax and Cleaved Caspase-3 (C caspase-3). ECM1 was the target gene of miR-486-3p, and miR-486-3p inhibited the expression of ECM1. Furthermore, ECM1 partially reversed the inhibitory effect of miR-486-3p on the proliferation, migration and invasion of RB cells. MiR-486-3p inhibited the proliferation, migration and invasion of RB by down-regulating ECM1. |
format | Online Article Text |
id | pubmed-7273916 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-72739162020-06-16 MiR-486-3p inhibits the proliferation, migration and invasion of retinoblastoma cells by targeting ECM1 Yang, Hongwei Huang, Yonggang He, Jian Chai, Guangrui Di, Yu Wang, Aiyuan Gui, Dongmei Biosci Rep Cancer It has been reported that miR-486-3p expression is decreased in retinoblastoma (RB) tumor tissues, however, its function in RB has been less reported. The present study aimed to explore the regulatory effects of miR-486-3p on RB cells. The expression of miR-486-3p in RB tissues and cells was detected by quantitative real-time polymerase chain reaction (qRT-PCR). Cell viability, proliferation, apoptosis, migration and invasion ability were determined by cell counting kit-8 (CCK-8) kit, clone formation assay, flow cytometry, scratch assay and transwell, respectively. Targetscan 7.2 and dual-luciferase reporter were used to verify target genes for miR-486-3p. The expressions of apoptosis-related proteins and ECM1 were detected by Western blot. The miR-486-3p expression was decreased in RB tissues and cells. In RB cells, overexpression of miR-486-3p inhibited cell proliferation, migration and invasion, while promoted apoptosis. Moreover, overexpression of miR-486-3p decreased Bcl-2 expression, while increased the expressions of Bax and Cleaved Caspase-3 (C caspase-3). ECM1 was the target gene of miR-486-3p, and miR-486-3p inhibited the expression of ECM1. Furthermore, ECM1 partially reversed the inhibitory effect of miR-486-3p on the proliferation, migration and invasion of RB cells. MiR-486-3p inhibited the proliferation, migration and invasion of RB by down-regulating ECM1. Portland Press Ltd. 2020-06-04 /pmc/articles/PMC7273916/ /pubmed/32401301 http://dx.doi.org/10.1042/BSR20200392 Text en © 2020 The Author(s). https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY). |
spellingShingle | Cancer Yang, Hongwei Huang, Yonggang He, Jian Chai, Guangrui Di, Yu Wang, Aiyuan Gui, Dongmei MiR-486-3p inhibits the proliferation, migration and invasion of retinoblastoma cells by targeting ECM1 |
title | MiR-486-3p inhibits the proliferation, migration and invasion of retinoblastoma cells by targeting ECM1 |
title_full | MiR-486-3p inhibits the proliferation, migration and invasion of retinoblastoma cells by targeting ECM1 |
title_fullStr | MiR-486-3p inhibits the proliferation, migration and invasion of retinoblastoma cells by targeting ECM1 |
title_full_unstemmed | MiR-486-3p inhibits the proliferation, migration and invasion of retinoblastoma cells by targeting ECM1 |
title_short | MiR-486-3p inhibits the proliferation, migration and invasion of retinoblastoma cells by targeting ECM1 |
title_sort | mir-486-3p inhibits the proliferation, migration and invasion of retinoblastoma cells by targeting ecm1 |
topic | Cancer |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7273916/ https://www.ncbi.nlm.nih.gov/pubmed/32401301 http://dx.doi.org/10.1042/BSR20200392 |
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