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Investigation of monoterpenoid resistance mechanisms in Pseudomonas putida and their consequences for biotransformations

Monoterpenoids are widely used in industrial applications, e.g. as active ingredients in pharmaceuticals, in flavor and fragrance compositions, and in agriculture. Severe toxic effects are known for some monoterpenoids making them challenging compounds for biotechnological production processes. Some...

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Autores principales: Schempp, Florence Miramella, Hofmann, Katharina Elisabeth, Mi, Jia, Kirchner, Ferdinand, Meffert, Annika, Schewe, Hendrik, Schrader, Jens, Buchhaupt, Markus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7275096/
https://www.ncbi.nlm.nih.gov/pubmed/32296906
http://dx.doi.org/10.1007/s00253-020-10566-3
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author Schempp, Florence Miramella
Hofmann, Katharina Elisabeth
Mi, Jia
Kirchner, Ferdinand
Meffert, Annika
Schewe, Hendrik
Schrader, Jens
Buchhaupt, Markus
author_facet Schempp, Florence Miramella
Hofmann, Katharina Elisabeth
Mi, Jia
Kirchner, Ferdinand
Meffert, Annika
Schewe, Hendrik
Schrader, Jens
Buchhaupt, Markus
author_sort Schempp, Florence Miramella
collection PubMed
description Monoterpenoids are widely used in industrial applications, e.g. as active ingredients in pharmaceuticals, in flavor and fragrance compositions, and in agriculture. Severe toxic effects are known for some monoterpenoids making them challenging compounds for biotechnological production processes. Some strains of the bacterium Pseudomonas putida show an inherent extraordinarily high tolerance towards solvents including monoterpenoids. An understanding of the underlying factors can help to create suitable strains for monoterpenoids de novo production or conversion. In addition, knowledge about tolerance mechanisms could allow a deeper insight into how bacteria can oppose monoterpenoid containing drugs, like tea tree oil. Within this work, the resistance mechanisms of P. putida GS1 were investigated using selected monoterpenoid-hypertolerant mutants. Most of the mutations were found in efflux pump promoter regions or associated transcription factors. Surprisingly, while for the tested monoterpenoid alcohols, ketone, and ether high efflux pump expression increased monoterpenoid tolerance, it reduced the tolerance against geranic acid. However, an increase of geranic acid tolerance could be gained by a mutation in an efflux pump component. It was also found that increased monoterpenoid tolerance can counteract efficient biotransformation ability, indicating the need for a fine-tuned and knowledge-based tolerance improvement for production strain development. Key points • Altered monoterpenoid tolerance mainly related to altered activity of efflux pumps. • Increased tolerance to geranic acid surprisingly caused by decreased export activity. • Reduction of export activity can be beneficial for biotechnological conversions. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00253-020-10566-3) contains supplementary material, which is available to authorized users.
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spelling pubmed-72750962020-06-16 Investigation of monoterpenoid resistance mechanisms in Pseudomonas putida and their consequences for biotransformations Schempp, Florence Miramella Hofmann, Katharina Elisabeth Mi, Jia Kirchner, Ferdinand Meffert, Annika Schewe, Hendrik Schrader, Jens Buchhaupt, Markus Appl Microbiol Biotechnol Applied Microbial and Cell Physiology Monoterpenoids are widely used in industrial applications, e.g. as active ingredients in pharmaceuticals, in flavor and fragrance compositions, and in agriculture. Severe toxic effects are known for some monoterpenoids making them challenging compounds for biotechnological production processes. Some strains of the bacterium Pseudomonas putida show an inherent extraordinarily high tolerance towards solvents including monoterpenoids. An understanding of the underlying factors can help to create suitable strains for monoterpenoids de novo production or conversion. In addition, knowledge about tolerance mechanisms could allow a deeper insight into how bacteria can oppose monoterpenoid containing drugs, like tea tree oil. Within this work, the resistance mechanisms of P. putida GS1 were investigated using selected monoterpenoid-hypertolerant mutants. Most of the mutations were found in efflux pump promoter regions or associated transcription factors. Surprisingly, while for the tested monoterpenoid alcohols, ketone, and ether high efflux pump expression increased monoterpenoid tolerance, it reduced the tolerance against geranic acid. However, an increase of geranic acid tolerance could be gained by a mutation in an efflux pump component. It was also found that increased monoterpenoid tolerance can counteract efficient biotransformation ability, indicating the need for a fine-tuned and knowledge-based tolerance improvement for production strain development. Key points • Altered monoterpenoid tolerance mainly related to altered activity of efflux pumps. • Increased tolerance to geranic acid surprisingly caused by decreased export activity. • Reduction of export activity can be beneficial for biotechnological conversions. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00253-020-10566-3) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2020-04-16 2020 /pmc/articles/PMC7275096/ /pubmed/32296906 http://dx.doi.org/10.1007/s00253-020-10566-3 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Applied Microbial and Cell Physiology
Schempp, Florence Miramella
Hofmann, Katharina Elisabeth
Mi, Jia
Kirchner, Ferdinand
Meffert, Annika
Schewe, Hendrik
Schrader, Jens
Buchhaupt, Markus
Investigation of monoterpenoid resistance mechanisms in Pseudomonas putida and their consequences for biotransformations
title Investigation of monoterpenoid resistance mechanisms in Pseudomonas putida and their consequences for biotransformations
title_full Investigation of monoterpenoid resistance mechanisms in Pseudomonas putida and their consequences for biotransformations
title_fullStr Investigation of monoterpenoid resistance mechanisms in Pseudomonas putida and their consequences for biotransformations
title_full_unstemmed Investigation of monoterpenoid resistance mechanisms in Pseudomonas putida and their consequences for biotransformations
title_short Investigation of monoterpenoid resistance mechanisms in Pseudomonas putida and their consequences for biotransformations
title_sort investigation of monoterpenoid resistance mechanisms in pseudomonas putida and their consequences for biotransformations
topic Applied Microbial and Cell Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7275096/
https://www.ncbi.nlm.nih.gov/pubmed/32296906
http://dx.doi.org/10.1007/s00253-020-10566-3
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