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Vital members in the gut microbiotas altered by two probiotic Bifidobacterium strains against liver damage in rats

BACKGROUND: Probiotics are effective to rectify the imbalanced gut microbiota in the diseased cohorts. Two Bifidobacterium strains (LI09 and LI10) were found to alleviate D-galactosamine-induced liver damage (LD) in rats in our previous work. A series of bioinformatic and statistical analyses were p...

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Autores principales: Zha, Hua, Fang, Dai-Qiong, van der Reis, Aimee, Chang, Kevin, Yang, Li-Ya, Xie, Jiao-Jiao, Shi, Ding, Xu, Qiao-Mai, Li, Ya-Ting, Li, Lan-Juan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7275491/
https://www.ncbi.nlm.nih.gov/pubmed/32503418
http://dx.doi.org/10.1186/s12866-020-01827-2
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author Zha, Hua
Fang, Dai-Qiong
van der Reis, Aimee
Chang, Kevin
Yang, Li-Ya
Xie, Jiao-Jiao
Shi, Ding
Xu, Qiao-Mai
Li, Ya-Ting
Li, Lan-Juan
author_facet Zha, Hua
Fang, Dai-Qiong
van der Reis, Aimee
Chang, Kevin
Yang, Li-Ya
Xie, Jiao-Jiao
Shi, Ding
Xu, Qiao-Mai
Li, Ya-Ting
Li, Lan-Juan
author_sort Zha, Hua
collection PubMed
description BACKGROUND: Probiotics are effective to rectify the imbalanced gut microbiota in the diseased cohorts. Two Bifidobacterium strains (LI09 and LI10) were found to alleviate D-galactosamine-induced liver damage (LD) in rats in our previous work. A series of bioinformatic and statistical analyses were performed to determine the vital bacteria in the gut microbiotas altered by the LI09 or LI10 in rats. RESULTS: Two groups of representative phylotypes could distinguish the gut microbiotas of LI09 or LI10 groups from the other groups. Among them, OTU170_Porphyromonadaceae acted as a gatekeeper in LI09 group, while OTU12_Bacteroides was determined with multiple correlations in the gut network of LI10 group. Multiple reduced OTUs associated with LC and increased OTUs associated with health were determined in LI09 or LI10 groups, among which, increased OTU51_Barnesiella and reduced OTU99_Barnesiella could be associated with the protective effects of both the two probiotics. The gut microbiotas in LI09, LI10 and positive control groups were clustered into three clusters, i.e., Cluster_1_Microbiota, Cluster_2_Microbiota and Cluster_3_Microbiota, by Partition Around Medoids clustering analysis. Cluster_2_Microbiota was determined at least dysbiotic status due to its greatest LD dysbiosis ratio, lowest levels of liver function variables and plasma cytokines compared with the two other clustered microbiotas, suggesting the treated rats in Cluster_2 were at better health status. CONCLUSION: Our findings suggest that OTU170_Porphyromonadaceae and OTU12_Bacteroides are vital in the gut microbiotas altered by LI09 and LI10. Characteristics of the LD cohorts treated by LI09 or LI10 at different gut microbial colonization states could help monitor the cohorts’ health status.
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spelling pubmed-72754912020-06-08 Vital members in the gut microbiotas altered by two probiotic Bifidobacterium strains against liver damage in rats Zha, Hua Fang, Dai-Qiong van der Reis, Aimee Chang, Kevin Yang, Li-Ya Xie, Jiao-Jiao Shi, Ding Xu, Qiao-Mai Li, Ya-Ting Li, Lan-Juan BMC Microbiol Research Article BACKGROUND: Probiotics are effective to rectify the imbalanced gut microbiota in the diseased cohorts. Two Bifidobacterium strains (LI09 and LI10) were found to alleviate D-galactosamine-induced liver damage (LD) in rats in our previous work. A series of bioinformatic and statistical analyses were performed to determine the vital bacteria in the gut microbiotas altered by the LI09 or LI10 in rats. RESULTS: Two groups of representative phylotypes could distinguish the gut microbiotas of LI09 or LI10 groups from the other groups. Among them, OTU170_Porphyromonadaceae acted as a gatekeeper in LI09 group, while OTU12_Bacteroides was determined with multiple correlations in the gut network of LI10 group. Multiple reduced OTUs associated with LC and increased OTUs associated with health were determined in LI09 or LI10 groups, among which, increased OTU51_Barnesiella and reduced OTU99_Barnesiella could be associated with the protective effects of both the two probiotics. The gut microbiotas in LI09, LI10 and positive control groups were clustered into three clusters, i.e., Cluster_1_Microbiota, Cluster_2_Microbiota and Cluster_3_Microbiota, by Partition Around Medoids clustering analysis. Cluster_2_Microbiota was determined at least dysbiotic status due to its greatest LD dysbiosis ratio, lowest levels of liver function variables and plasma cytokines compared with the two other clustered microbiotas, suggesting the treated rats in Cluster_2 were at better health status. CONCLUSION: Our findings suggest that OTU170_Porphyromonadaceae and OTU12_Bacteroides are vital in the gut microbiotas altered by LI09 and LI10. Characteristics of the LD cohorts treated by LI09 or LI10 at different gut microbial colonization states could help monitor the cohorts’ health status. BioMed Central 2020-06-05 /pmc/articles/PMC7275491/ /pubmed/32503418 http://dx.doi.org/10.1186/s12866-020-01827-2 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Zha, Hua
Fang, Dai-Qiong
van der Reis, Aimee
Chang, Kevin
Yang, Li-Ya
Xie, Jiao-Jiao
Shi, Ding
Xu, Qiao-Mai
Li, Ya-Ting
Li, Lan-Juan
Vital members in the gut microbiotas altered by two probiotic Bifidobacterium strains against liver damage in rats
title Vital members in the gut microbiotas altered by two probiotic Bifidobacterium strains against liver damage in rats
title_full Vital members in the gut microbiotas altered by two probiotic Bifidobacterium strains against liver damage in rats
title_fullStr Vital members in the gut microbiotas altered by two probiotic Bifidobacterium strains against liver damage in rats
title_full_unstemmed Vital members in the gut microbiotas altered by two probiotic Bifidobacterium strains against liver damage in rats
title_short Vital members in the gut microbiotas altered by two probiotic Bifidobacterium strains against liver damage in rats
title_sort vital members in the gut microbiotas altered by two probiotic bifidobacterium strains against liver damage in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7275491/
https://www.ncbi.nlm.nih.gov/pubmed/32503418
http://dx.doi.org/10.1186/s12866-020-01827-2
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