LncRNA FLVCR1-AS1 promotes proliferation, migration and activates Wnt/β-catenin pathway through miR-381-3p/CTNNB1 axis in breast cancer

BACKGROUND: Understanding the molecular mechanism of long non-coding RNAs (lncRNAs) in carcinogenesis is conducive for providing potential target for cancers. The role of FLVCR1-AS1 in breast cancer (BC) has not been probed yet. MATERIALS AND METHODS: qRT-PCR and western blot assays were used to est...

Descripción completa

Detalles Bibliográficos
Autores principales: Pan, Zhiyu, Ding, Junbin, Yang, Zhen, Li, Huaqing, Ding, Hongjian, Chen, Qian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Primary Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7275497/
https://www.ncbi.nlm.nih.gov/pubmed/32518523
http://dx.doi.org/10.1186/s12935-020-01247-2
_version_ 1783542796906397696
author Pan, Zhiyu
Ding, Junbin
Yang, Zhen
Li, Huaqing
Ding, Hongjian
Chen, Qian
author_facet Pan, Zhiyu
Ding, Junbin
Yang, Zhen
Li, Huaqing
Ding, Hongjian
Chen, Qian
author_sort Pan, Zhiyu
collection PubMed
description BACKGROUND: Understanding the molecular mechanism of long non-coding RNAs (lncRNAs) in carcinogenesis is conducive for providing potential target for cancers. The role of FLVCR1-AS1 in breast cancer (BC) has not been probed yet. MATERIALS AND METHODS: qRT-PCR and western blot assays were used to estimate relevant expressions of mRNAs and proteins. CCK8, MTT and EdU were implemented to assess cell proliferation ability. TUNEL was performed to investigate cell apoptosis, whereas transwell assay was performed to test cell migration and invasion capacities. TOP/FOP Flash assay was conducted to determine the activity of Wnt/β-catenin pathway. Luciferase reporter, RNA pull down and RIP assays were performed to verify interaction between genes. RESULTS: FLVCR1-AS1 was abnormally up-regulated in BC cells. Silencing FLVCR1-AS1 inhibited cell proliferation, migration, invasion, yet accelerating apoptosis. Inhibition of miR-381-3p reversed the tumor restraining impacts of FLVCR1-AS1 depletion on BC progression. Additionally, CTNNB1 was recognized to be targeted by miR-381-3p. FLVCR1-AS1 aggravated BC malignant progression via up-regulation CTNNB1 through sponging miR-381-3p. CONCLUSION: FLVCR1-AS1 regulates BC malignant behavior via sequestering miR-381-3p and then freeing CTNNB1, implying a promising target for BC therapy.
format Online
Article
Text
id pubmed-7275497
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-72754972020-06-08 LncRNA FLVCR1-AS1 promotes proliferation, migration and activates Wnt/β-catenin pathway through miR-381-3p/CTNNB1 axis in breast cancer Pan, Zhiyu Ding, Junbin Yang, Zhen Li, Huaqing Ding, Hongjian Chen, Qian Cancer Cell Int Primary Research BACKGROUND: Understanding the molecular mechanism of long non-coding RNAs (lncRNAs) in carcinogenesis is conducive for providing potential target for cancers. The role of FLVCR1-AS1 in breast cancer (BC) has not been probed yet. MATERIALS AND METHODS: qRT-PCR and western blot assays were used to estimate relevant expressions of mRNAs and proteins. CCK8, MTT and EdU were implemented to assess cell proliferation ability. TUNEL was performed to investigate cell apoptosis, whereas transwell assay was performed to test cell migration and invasion capacities. TOP/FOP Flash assay was conducted to determine the activity of Wnt/β-catenin pathway. Luciferase reporter, RNA pull down and RIP assays were performed to verify interaction between genes. RESULTS: FLVCR1-AS1 was abnormally up-regulated in BC cells. Silencing FLVCR1-AS1 inhibited cell proliferation, migration, invasion, yet accelerating apoptosis. Inhibition of miR-381-3p reversed the tumor restraining impacts of FLVCR1-AS1 depletion on BC progression. Additionally, CTNNB1 was recognized to be targeted by miR-381-3p. FLVCR1-AS1 aggravated BC malignant progression via up-regulation CTNNB1 through sponging miR-381-3p. CONCLUSION: FLVCR1-AS1 regulates BC malignant behavior via sequestering miR-381-3p and then freeing CTNNB1, implying a promising target for BC therapy. BioMed Central 2020-06-05 /pmc/articles/PMC7275497/ /pubmed/32518523 http://dx.doi.org/10.1186/s12935-020-01247-2 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Primary Research
Pan, Zhiyu
Ding, Junbin
Yang, Zhen
Li, Huaqing
Ding, Hongjian
Chen, Qian
LncRNA FLVCR1-AS1 promotes proliferation, migration and activates Wnt/β-catenin pathway through miR-381-3p/CTNNB1 axis in breast cancer
title LncRNA FLVCR1-AS1 promotes proliferation, migration and activates Wnt/β-catenin pathway through miR-381-3p/CTNNB1 axis in breast cancer
title_full LncRNA FLVCR1-AS1 promotes proliferation, migration and activates Wnt/β-catenin pathway through miR-381-3p/CTNNB1 axis in breast cancer
title_fullStr LncRNA FLVCR1-AS1 promotes proliferation, migration and activates Wnt/β-catenin pathway through miR-381-3p/CTNNB1 axis in breast cancer
title_full_unstemmed LncRNA FLVCR1-AS1 promotes proliferation, migration and activates Wnt/β-catenin pathway through miR-381-3p/CTNNB1 axis in breast cancer
title_short LncRNA FLVCR1-AS1 promotes proliferation, migration and activates Wnt/β-catenin pathway through miR-381-3p/CTNNB1 axis in breast cancer
title_sort lncrna flvcr1-as1 promotes proliferation, migration and activates wnt/β-catenin pathway through mir-381-3p/ctnnb1 axis in breast cancer
topic Primary Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7275497/
https://www.ncbi.nlm.nih.gov/pubmed/32518523
http://dx.doi.org/10.1186/s12935-020-01247-2
work_keys_str_mv AT panzhiyu lncrnaflvcr1as1promotesproliferationmigrationandactivateswntbcateninpathwaythroughmir3813pctnnb1axisinbreastcancer
AT dingjunbin lncrnaflvcr1as1promotesproliferationmigrationandactivateswntbcateninpathwaythroughmir3813pctnnb1axisinbreastcancer
AT yangzhen lncrnaflvcr1as1promotesproliferationmigrationandactivateswntbcateninpathwaythroughmir3813pctnnb1axisinbreastcancer
AT lihuaqing lncrnaflvcr1as1promotesproliferationmigrationandactivateswntbcateninpathwaythroughmir3813pctnnb1axisinbreastcancer
AT dinghongjian lncrnaflvcr1as1promotesproliferationmigrationandactivateswntbcateninpathwaythroughmir3813pctnnb1axisinbreastcancer
AT chenqian lncrnaflvcr1as1promotesproliferationmigrationandactivateswntbcateninpathwaythroughmir3813pctnnb1axisinbreastcancer

Ejemplares similares