Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of SARS-CoV-2

BACKGROUND: Highly sensitive real-time reverse transcription polymerase chain reaction (RT-qPCR) methods have been developed for the detection of SARS-CoV-2. However, they are costly. Loop-mediated isothermal amplification (LAMP) assay has emerged as a novel alternative isothermal amplification meth...

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Detalles Bibliográficos
Autores principales: Lau, Yee Ling, Ismail, Ilyiana, Mustapa, Nur Izati, Lai, Meng Yee, Tuan Soh, Tuan Suhaila, Hassan, Afifah, Peariasamy, Kalaiarasu M., Lee, Yee Leng, Chong, Yoong Min, Sam, I-Ching, Goh, Pik Pin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2020
Materias:
Virology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7275676/
https://www.ncbi.nlm.nih.gov/pubmed/32547882
http://dx.doi.org/10.7717/peerj.9278
Descripción
Sumario:BACKGROUND: Highly sensitive real-time reverse transcription polymerase chain reaction (RT-qPCR) methods have been developed for the detection of SARS-CoV-2. However, they are costly. Loop-mediated isothermal amplification (LAMP) assay has emerged as a novel alternative isothermal amplification method for the detection of nucleic acid. METHODS: A rapid, sensitive and specific real-time reverse transcription LAMP (RT-LAMP) assay was developed for SARS-CoV-2 detection. RESULTS: This assay detected one copy/reaction of SARS-CoV-2 RNA in 30 min. Both the clinical sensitivity and specificity of this assay were 100%. The RT-LAMP showed comparable performance with RT-qPCR. Combining simplicity and cost-effectiveness, this assay is therefore recommended for use in resource resource-limited settings.

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