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Enhanced Adipose Mesenchymal Stem Cells Proliferation by Carboxymethyl-Chitosan Functionalized Polycaprolactone Nanofiber

BACKGROUND: Through combining two synthetic and natural polymers, scaffolds can be developed for tissue engineering and regenerative medicine purposes. METHODS: In this work, CMC (20%) was grafted to PCL nanofibers using the cold atmospheric plasma of helium. The PCL scaffolds were exposed to CAP, a...

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Autores principales: Shapourzadeh, Atena, Atyabi, Seyed Mohammad, Irani, Shiva, Bakhshi, Hadi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pasteur Institute of Iran 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7275816/
https://www.ncbi.nlm.nih.gov/pubmed/32306721
http://dx.doi.org/10.29252/ibj.24.4.236
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author Shapourzadeh, Atena
Atyabi, Seyed Mohammad
Irani, Shiva
Bakhshi, Hadi
author_facet Shapourzadeh, Atena
Atyabi, Seyed Mohammad
Irani, Shiva
Bakhshi, Hadi
author_sort Shapourzadeh, Atena
collection PubMed
description BACKGROUND: Through combining two synthetic and natural polymers, scaffolds can be developed for tissue engineering and regenerative medicine purposes. METHODS: In this work, CMC (20%) was grafted to PCL nanofibers using the cold atmospheric plasma of helium. The PCL scaffolds were exposed to CAP, and functional groups were developed on the PCL surface. RESULTS: The results of FTIR confirmed CMC (20%) graft on PCL scaffold. The MTT assay showed a significant enhancement (p < 0.05) in the cell affinity and proliferation of ADSCs to CMC20%-graft-PCL scaffolds. After 14 days, bone differentiation was affirmed through alizarin red and calcium depositions. CONCLUSION: Based on the results, the CMC20%-graft-PCL can support the proliferation of ADSCs and induce the differentiation into bone with longer culture time.
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spelling pubmed-72758162020-07-01 Enhanced Adipose Mesenchymal Stem Cells Proliferation by Carboxymethyl-Chitosan Functionalized Polycaprolactone Nanofiber Shapourzadeh, Atena Atyabi, Seyed Mohammad Irani, Shiva Bakhshi, Hadi Iran Biomed J Full Length BACKGROUND: Through combining two synthetic and natural polymers, scaffolds can be developed for tissue engineering and regenerative medicine purposes. METHODS: In this work, CMC (20%) was grafted to PCL nanofibers using the cold atmospheric plasma of helium. The PCL scaffolds were exposed to CAP, and functional groups were developed on the PCL surface. RESULTS: The results of FTIR confirmed CMC (20%) graft on PCL scaffold. The MTT assay showed a significant enhancement (p < 0.05) in the cell affinity and proliferation of ADSCs to CMC20%-graft-PCL scaffolds. After 14 days, bone differentiation was affirmed through alizarin red and calcium depositions. CONCLUSION: Based on the results, the CMC20%-graft-PCL can support the proliferation of ADSCs and induce the differentiation into bone with longer culture time. Pasteur Institute of Iran 2020-07 2020-02-12 /pmc/articles/PMC7275816/ /pubmed/32306721 http://dx.doi.org/10.29252/ibj.24.4.236 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Full Length
Shapourzadeh, Atena
Atyabi, Seyed Mohammad
Irani, Shiva
Bakhshi, Hadi
Enhanced Adipose Mesenchymal Stem Cells Proliferation by Carboxymethyl-Chitosan Functionalized Polycaprolactone Nanofiber
title Enhanced Adipose Mesenchymal Stem Cells Proliferation by Carboxymethyl-Chitosan Functionalized Polycaprolactone Nanofiber
title_full Enhanced Adipose Mesenchymal Stem Cells Proliferation by Carboxymethyl-Chitosan Functionalized Polycaprolactone Nanofiber
title_fullStr Enhanced Adipose Mesenchymal Stem Cells Proliferation by Carboxymethyl-Chitosan Functionalized Polycaprolactone Nanofiber
title_full_unstemmed Enhanced Adipose Mesenchymal Stem Cells Proliferation by Carboxymethyl-Chitosan Functionalized Polycaprolactone Nanofiber
title_short Enhanced Adipose Mesenchymal Stem Cells Proliferation by Carboxymethyl-Chitosan Functionalized Polycaprolactone Nanofiber
title_sort enhanced adipose mesenchymal stem cells proliferation by carboxymethyl-chitosan functionalized polycaprolactone nanofiber
topic Full Length
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7275816/
https://www.ncbi.nlm.nih.gov/pubmed/32306721
http://dx.doi.org/10.29252/ibj.24.4.236
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