A multiplex qPCR TaqMan-assay to detect fungal antagonism between Trichoderma atroviride (Hypocreaceae) and Botrytis cinerea (Sclerotiniaceae) in blackberry fruits using a de novo tef1-α- and an IGS-sequence based probes

The aim of this study was to design a Trichoderma atroviride-specific qPCR oligo set, evaluate its specificity, and standardize a methodology that quantifies antagonism against Botrytis cinerea in blackberry fruits (Rubus adenotrichos Schltdl.). Primers and probe were designed based on the nuclear t...

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Detalles Bibliográficos
Autores principales: Hilje-Rodríguez, Irena, Albertazzi, Federico J., Rivera-Coto, German, Molina-Bravo, Ramón
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7276392/
https://www.ncbi.nlm.nih.gov/pubmed/32528864
http://dx.doi.org/10.1016/j.btre.2020.e00447

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