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Adsorption of a styrene maleic acid (SMA) copolymer-stabilized phospholipid nanodisc on a solid-supported planar lipid bilayer
Over recent years, there has been a rapid development of membrane-mimetic systems to encapsulate and stabilize planar segments of phospholipid bilayers in solution. One such system has been the use of amphipathic copolymers to solubilize lipid bilayers into nanodiscs. The attractiveness of this syst...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Academic Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7276985/ https://www.ncbi.nlm.nih.gov/pubmed/32330753 http://dx.doi.org/10.1016/j.jcis.2020.04.013 |
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author | Hall, Stephen C.L. Clifton, Luke A. Tognoloni, Cecilia Morrison, Kerrie A. Knowles, Timothy J. Kinane, Christian J. Dafforn, Tim R. Edler, Karen J. Arnold, Thomas |
author_facet | Hall, Stephen C.L. Clifton, Luke A. Tognoloni, Cecilia Morrison, Kerrie A. Knowles, Timothy J. Kinane, Christian J. Dafforn, Tim R. Edler, Karen J. Arnold, Thomas |
author_sort | Hall, Stephen C.L. |
collection | PubMed |
description | Over recent years, there has been a rapid development of membrane-mimetic systems to encapsulate and stabilize planar segments of phospholipid bilayers in solution. One such system has been the use of amphipathic copolymers to solubilize lipid bilayers into nanodiscs. The attractiveness of this system, in part, stems from the capability of these polymers to solubilize membrane proteins directly from the host cell membrane. The assumption has been that the native lipid annulus remains intact, with nanodiscs providing a snapshot of the lipid environment. Recent studies have provided evidence that phospholipids can exchange from the nanodiscs with either lipids at interfaces, or with other nanodiscs in bulk solution. Here we investigate kinetics of lipid exchange between three recently studied polymer-stabilized nanodiscs and supported lipid bilayers at the silicon-water interface. We show that lipid and polymer exchange occurs in all nanodiscs tested, although the rate and extent differs between different nanodisc types. Furthermore, we observe adsorption of nanodiscs to the supported lipid bilayer for one nanodisc system which used a polymer made using reversible addition-fragmentation chain transfer polymerization. These results have important implications in applications of polymer-stabilized nanodiscs, such as in the fabrication of solid-supported films containing membrane proteins. |
format | Online Article Text |
id | pubmed-7276985 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Academic Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-72769852020-08-15 Adsorption of a styrene maleic acid (SMA) copolymer-stabilized phospholipid nanodisc on a solid-supported planar lipid bilayer Hall, Stephen C.L. Clifton, Luke A. Tognoloni, Cecilia Morrison, Kerrie A. Knowles, Timothy J. Kinane, Christian J. Dafforn, Tim R. Edler, Karen J. Arnold, Thomas J Colloid Interface Sci Article Over recent years, there has been a rapid development of membrane-mimetic systems to encapsulate and stabilize planar segments of phospholipid bilayers in solution. One such system has been the use of amphipathic copolymers to solubilize lipid bilayers into nanodiscs. The attractiveness of this system, in part, stems from the capability of these polymers to solubilize membrane proteins directly from the host cell membrane. The assumption has been that the native lipid annulus remains intact, with nanodiscs providing a snapshot of the lipid environment. Recent studies have provided evidence that phospholipids can exchange from the nanodiscs with either lipids at interfaces, or with other nanodiscs in bulk solution. Here we investigate kinetics of lipid exchange between three recently studied polymer-stabilized nanodiscs and supported lipid bilayers at the silicon-water interface. We show that lipid and polymer exchange occurs in all nanodiscs tested, although the rate and extent differs between different nanodisc types. Furthermore, we observe adsorption of nanodiscs to the supported lipid bilayer for one nanodisc system which used a polymer made using reversible addition-fragmentation chain transfer polymerization. These results have important implications in applications of polymer-stabilized nanodiscs, such as in the fabrication of solid-supported films containing membrane proteins. Academic Press 2020-08-15 /pmc/articles/PMC7276985/ /pubmed/32330753 http://dx.doi.org/10.1016/j.jcis.2020.04.013 Text en © 2020 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Hall, Stephen C.L. Clifton, Luke A. Tognoloni, Cecilia Morrison, Kerrie A. Knowles, Timothy J. Kinane, Christian J. Dafforn, Tim R. Edler, Karen J. Arnold, Thomas Adsorption of a styrene maleic acid (SMA) copolymer-stabilized phospholipid nanodisc on a solid-supported planar lipid bilayer |
title | Adsorption of a styrene maleic acid (SMA) copolymer-stabilized phospholipid nanodisc on a solid-supported planar lipid bilayer |
title_full | Adsorption of a styrene maleic acid (SMA) copolymer-stabilized phospholipid nanodisc on a solid-supported planar lipid bilayer |
title_fullStr | Adsorption of a styrene maleic acid (SMA) copolymer-stabilized phospholipid nanodisc on a solid-supported planar lipid bilayer |
title_full_unstemmed | Adsorption of a styrene maleic acid (SMA) copolymer-stabilized phospholipid nanodisc on a solid-supported planar lipid bilayer |
title_short | Adsorption of a styrene maleic acid (SMA) copolymer-stabilized phospholipid nanodisc on a solid-supported planar lipid bilayer |
title_sort | adsorption of a styrene maleic acid (sma) copolymer-stabilized phospholipid nanodisc on a solid-supported planar lipid bilayer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7276985/ https://www.ncbi.nlm.nih.gov/pubmed/32330753 http://dx.doi.org/10.1016/j.jcis.2020.04.013 |
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