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Recent Progress on the Electrochemical Biosensing of Escherichia coli O157:H7: Material and Methods Overview
Escherichia coli O157:H7 (E. coli O157:H7) is a pathogenic strain of Escherichia coli which has issued as a public health threat because of fatal contamination of food and water. Therefore, accurate detection of pathogenic E. coli is important in environmental and food quality monitoring. In spite o...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7277213/ https://www.ncbi.nlm.nih.gov/pubmed/32443629 http://dx.doi.org/10.3390/bios10050054 |
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author | Razmi, Nasrin Hasanzadeh, Mohammad Willander, Magnus Nur, Omer |
author_facet | Razmi, Nasrin Hasanzadeh, Mohammad Willander, Magnus Nur, Omer |
author_sort | Razmi, Nasrin |
collection | PubMed |
description | Escherichia coli O157:H7 (E. coli O157:H7) is a pathogenic strain of Escherichia coli which has issued as a public health threat because of fatal contamination of food and water. Therefore, accurate detection of pathogenic E. coli is important in environmental and food quality monitoring. In spite of their advantages and high acceptance, culture-based methods, enzyme-linked immunosorbent assays (ELISAs), polymerase chain reaction (PCR), flow cytometry, ATP bioluminescence, and solid-phase cytometry have various drawbacks, including being time-consuming, requiring trained technicians and/or specific equipment, and producing biological waste. Therefore, there is necessity for affordable, rapid, and simple approaches. Electrochemical biosensors have shown great promise for rapid food- and water-borne pathogen detection. Over the last decade, various attempts have been made to develop techniques for the rapid quantification of E. coli O157:H7. This review covers the importance of E. coli O157:H7 and recent progress (from 2015 to 2020) in the development of the sensitivity and selectivity of electrochemical sensors developed for E. coli O157:H7 using different nanomaterials, labels, and electrochemical transducers. |
format | Online Article Text |
id | pubmed-7277213 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72772132020-06-15 Recent Progress on the Electrochemical Biosensing of Escherichia coli O157:H7: Material and Methods Overview Razmi, Nasrin Hasanzadeh, Mohammad Willander, Magnus Nur, Omer Biosensors (Basel) Review Escherichia coli O157:H7 (E. coli O157:H7) is a pathogenic strain of Escherichia coli which has issued as a public health threat because of fatal contamination of food and water. Therefore, accurate detection of pathogenic E. coli is important in environmental and food quality monitoring. In spite of their advantages and high acceptance, culture-based methods, enzyme-linked immunosorbent assays (ELISAs), polymerase chain reaction (PCR), flow cytometry, ATP bioluminescence, and solid-phase cytometry have various drawbacks, including being time-consuming, requiring trained technicians and/or specific equipment, and producing biological waste. Therefore, there is necessity for affordable, rapid, and simple approaches. Electrochemical biosensors have shown great promise for rapid food- and water-borne pathogen detection. Over the last decade, various attempts have been made to develop techniques for the rapid quantification of E. coli O157:H7. This review covers the importance of E. coli O157:H7 and recent progress (from 2015 to 2020) in the development of the sensitivity and selectivity of electrochemical sensors developed for E. coli O157:H7 using different nanomaterials, labels, and electrochemical transducers. MDPI 2020-05-18 /pmc/articles/PMC7277213/ /pubmed/32443629 http://dx.doi.org/10.3390/bios10050054 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Razmi, Nasrin Hasanzadeh, Mohammad Willander, Magnus Nur, Omer Recent Progress on the Electrochemical Biosensing of Escherichia coli O157:H7: Material and Methods Overview |
title | Recent Progress on the Electrochemical Biosensing of Escherichia coli O157:H7: Material and Methods Overview |
title_full | Recent Progress on the Electrochemical Biosensing of Escherichia coli O157:H7: Material and Methods Overview |
title_fullStr | Recent Progress on the Electrochemical Biosensing of Escherichia coli O157:H7: Material and Methods Overview |
title_full_unstemmed | Recent Progress on the Electrochemical Biosensing of Escherichia coli O157:H7: Material and Methods Overview |
title_short | Recent Progress on the Electrochemical Biosensing of Escherichia coli O157:H7: Material and Methods Overview |
title_sort | recent progress on the electrochemical biosensing of escherichia coli o157:h7: material and methods overview |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7277213/ https://www.ncbi.nlm.nih.gov/pubmed/32443629 http://dx.doi.org/10.3390/bios10050054 |
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