Behavioral Response of Weaned Pigs during Gas Euthanasia with CO(2), CO(2) with Butorphanol, or Nitrous Oxide

SIMPLE SUMMARY: Pig farmers are forced to euthanize a significant number of pigs due to injuries, hernias, or unthriftiness. The majority of pigs are euthanized using carbon dioxide gas asphyxiation. However, the humaneness of carbon dioxide is being increasingly questioned. An alternative is the use of nitrous oxide gas. We conducted this study to compare the euthanasia of young pigs using nitrous oxide or carbon dioxide. In addition, we tested the administration of a pain relief drug prior to carbon dioxide exposure to determine if we could eliminate behaviors indicative of pain. Pigs became unable to control their muscle movement, breathed heavily, and lost posture at the same time regardless of treatment. Pigs exposed to both gases showed heavy breathing and open-mouth breathing prior to losing posture. However, pigs exposed to carbon dioxide made more escape attempts but fewer squeals than pigs exposed to nitrous oxide. Administration of pain relief prior to exposure to carbon dioxide did not alter behaviors indicative of pain. The findings are inconclusive as to whether using nitrous oxide is significantly better than using carbon dioxide, but the results show that its use is just as effective, and possibly more humane. ABSTRACT: The swine industry is often forced to euthanize pigs in the first few weeks of life due to injuries, hernias, or unthriftiness. The majority of pigs are euthanized using carbon dioxide (CO(2)) gas asphyxiation but concerns as to the humaneness of CO(2) are increasing. This study compared the euthanasia of weaned pigs using N(2)O (N(2)O; n = 9) or CO(2) (n = 9), at 50% and 25% min(−1) exchange rate, respectively. In addition, we administered an analgesic prior to euthanasia with CO(2) (CO(2)B) exposure as a third treatment (n = 9) to elucidate behaviors indicative of pain. Pigs in the CO(2) and N(2)O treatments lost posture at similar times (latency of 145.0 ± 17.3 and 162.6 ± 7.0 s respectively, p > 0.10), while the CO(2)B treatment pigs lost posture the soonest (101.2 ± 4.7 s, p < 0.01). The pigs in the CO(2)B treatment made more escape attempts than the CO(2) or N(2)O pigs (16.4 ± 4.2, 4.7 ± 1.6, 0.3 ± 0.2, respectively; p < 0.0004). However, pigs in N(2)O squealed more often than either the CO(2) or CO(2)B pigs (9.0 ± 1.6, 2.8 ± 1.2, 1.3 ± 0.6, respectively, p < 0.001). Given the similar time to loss of posture and shorter time displaying open mouth breathing, N(2)O may cause less stress to pigs; however, the greater number of squeals performed by these pigs suggests the opposite. It was not apparent that any behavior measured was indicative of pain. In conclusion, N(2)O applied at a 50% min(−1) flow rate can be an alternative to CO(2) for pig euthanasia.