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Determination of Selenium Species in Muscle, Heart, and Liver Tissues of Lambs Using Mass Spectrometry Methods

SIMPLE SUMMARY: The objective of the project was to evaluate speciation of selenium (Se) in various tissues (the liver, heart, or muscles) obtained from lambs fed with a diet enriched with an inorganic and organic chemical form of Se (i.e., selenite and Se-enriched yeast, respectively). ABSTRACT: Id...

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Autores principales: Gawor, Andrzej, Ruszczynska, Anna, Czauderna, Marian, Bulska, Ewa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7277398/
https://www.ncbi.nlm.nih.gov/pubmed/32392714
http://dx.doi.org/10.3390/ani10050808
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author Gawor, Andrzej
Ruszczynska, Anna
Czauderna, Marian
Bulska, Ewa
author_facet Gawor, Andrzej
Ruszczynska, Anna
Czauderna, Marian
Bulska, Ewa
author_sort Gawor, Andrzej
collection PubMed
description SIMPLE SUMMARY: The objective of the project was to evaluate speciation of selenium (Se) in various tissues (the liver, heart, or muscles) obtained from lambs fed with a diet enriched with an inorganic and organic chemical form of Se (i.e., selenite and Se-enriched yeast, respectively). ABSTRACT: Identification and quantification of the selenium species in biological tissues is imperative, considering the need to properly understand its metabolism and its importance in various field of sciences, especially nutrition science. Although a number of studies deals with the speciation of selenium, speciation analysis is still far from being a routine task, and so far strongly depends on the type of the samples. We present a study aimed to examine speciation analysis of Se in tissues of livers, muscles, and hearts obtained from lambs, namely in liver, muscle, and heart. The studied lambs were fed with the diet enriched with an inorganic (as sodium selenate) and organic chemical form of Se (as Se-enriched yeast) compounds with simultaneous addition of fish oil (FO) and carnosic acid (CA). The first part of the work was focused on the optimization of the extraction procedure of selenium compounds from tissues. Next, hyphenated high performance liquid chromatography and inductively coupled plasma mass spectrometry (HPLC–ICP–MS) was used for the identification of five seleno-compounds—Se-methionine (SeMet), Se-cystine (SeCys2), Se-methyl-Se-cysteine (SeMetSeCys), and Se(IV) and Se(VI). Verification of the identified seleno-compounds was achieved using triple-quadrupole mass spectrometer coupled to high performance liquid chromatography (HPLC–ESI–MS/MS). The applied procedure allowed for quantitative analysis of SeMet, SeCys2, and SeMetSeCys, in biological tissues. The developed analytical protocol is feasible for speciation analysis of small molecular seleno-compounds in animals samples.
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spelling pubmed-72773982020-06-15 Determination of Selenium Species in Muscle, Heart, and Liver Tissues of Lambs Using Mass Spectrometry Methods Gawor, Andrzej Ruszczynska, Anna Czauderna, Marian Bulska, Ewa Animals (Basel) Article SIMPLE SUMMARY: The objective of the project was to evaluate speciation of selenium (Se) in various tissues (the liver, heart, or muscles) obtained from lambs fed with a diet enriched with an inorganic and organic chemical form of Se (i.e., selenite and Se-enriched yeast, respectively). ABSTRACT: Identification and quantification of the selenium species in biological tissues is imperative, considering the need to properly understand its metabolism and its importance in various field of sciences, especially nutrition science. Although a number of studies deals with the speciation of selenium, speciation analysis is still far from being a routine task, and so far strongly depends on the type of the samples. We present a study aimed to examine speciation analysis of Se in tissues of livers, muscles, and hearts obtained from lambs, namely in liver, muscle, and heart. The studied lambs were fed with the diet enriched with an inorganic (as sodium selenate) and organic chemical form of Se (as Se-enriched yeast) compounds with simultaneous addition of fish oil (FO) and carnosic acid (CA). The first part of the work was focused on the optimization of the extraction procedure of selenium compounds from tissues. Next, hyphenated high performance liquid chromatography and inductively coupled plasma mass spectrometry (HPLC–ICP–MS) was used for the identification of five seleno-compounds—Se-methionine (SeMet), Se-cystine (SeCys2), Se-methyl-Se-cysteine (SeMetSeCys), and Se(IV) and Se(VI). Verification of the identified seleno-compounds was achieved using triple-quadrupole mass spectrometer coupled to high performance liquid chromatography (HPLC–ESI–MS/MS). The applied procedure allowed for quantitative analysis of SeMet, SeCys2, and SeMetSeCys, in biological tissues. The developed analytical protocol is feasible for speciation analysis of small molecular seleno-compounds in animals samples. MDPI 2020-05-07 /pmc/articles/PMC7277398/ /pubmed/32392714 http://dx.doi.org/10.3390/ani10050808 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gawor, Andrzej
Ruszczynska, Anna
Czauderna, Marian
Bulska, Ewa
Determination of Selenium Species in Muscle, Heart, and Liver Tissues of Lambs Using Mass Spectrometry Methods
title Determination of Selenium Species in Muscle, Heart, and Liver Tissues of Lambs Using Mass Spectrometry Methods
title_full Determination of Selenium Species in Muscle, Heart, and Liver Tissues of Lambs Using Mass Spectrometry Methods
title_fullStr Determination of Selenium Species in Muscle, Heart, and Liver Tissues of Lambs Using Mass Spectrometry Methods
title_full_unstemmed Determination of Selenium Species in Muscle, Heart, and Liver Tissues of Lambs Using Mass Spectrometry Methods
title_short Determination of Selenium Species in Muscle, Heart, and Liver Tissues of Lambs Using Mass Spectrometry Methods
title_sort determination of selenium species in muscle, heart, and liver tissues of lambs using mass spectrometry methods
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7277398/
https://www.ncbi.nlm.nih.gov/pubmed/32392714
http://dx.doi.org/10.3390/ani10050808
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