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In Vitro Antibacterial and Anti-Inflammatory Effects of Novel Insect Fungus Polycephalomyces phaothaiensis Extract and Its Constituents against Propionibacterium acnes
Propionibacterium acnes plays an important role in the pathophysiology of acne vulgaris, the most common chronic inflammatory skin disease of the pilosebaceous unit. This study was conducted to investigate whether the entomopathogenic fungus Polycephalomyces phaothaiensis components have antibacteri...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7277416/ https://www.ncbi.nlm.nih.gov/pubmed/32466146 http://dx.doi.org/10.3390/antibiotics9050274 |
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author | Sonyot, Witsanu Lamlertthon, Supaporn Luangsa-ard, Janet Jennifer Mongkolsamrit, Suchada Usuwanthim, Kanchana Ingkaninan, Kornkanok Waranuch, Neti Suphrom, Nungruthai |
author_facet | Sonyot, Witsanu Lamlertthon, Supaporn Luangsa-ard, Janet Jennifer Mongkolsamrit, Suchada Usuwanthim, Kanchana Ingkaninan, Kornkanok Waranuch, Neti Suphrom, Nungruthai |
author_sort | Sonyot, Witsanu |
collection | PubMed |
description | Propionibacterium acnes plays an important role in the pathophysiology of acne vulgaris, the most common chronic inflammatory skin disease of the pilosebaceous unit. This study was conducted to investigate whether the entomopathogenic fungus Polycephalomyces phaothaiensis components have antibacterial and anti-inflammatory effects against P. acnes that may serve for acne treatment. A chemical study by spectroscopic analysis resulted in the identification of seven known compounds. The anti-P. acnes potency of extracts and test compounds was determined by both agar diffusion and broth dilution methods. The ethyl acetate extract from culture broth along with cordytropolone (1) and stipitalide (2) exhibited strong anti- P. acnes activity while (+)-piliformic acid (3) showed weak inhibitory activity. The anti-inflammatory effect of ethyl acetate extract and 1–3 was then examined by the quantification of pro-inflammatory cytokines IL-1β, IL-6, and TNF-α on heat-killed P. acnes induced cytokine production by THP-1 cells. The result demonstrated that the extract and its constituents (1–3) showed a potent significant effect by inhibiting the P. acnes-induced pro-inflammatory cytokines production in THP-1. Our results suggest for the first time that P. phaothaiensis and its constituents (1 and 2) hold therapeutic value for further studies as a new alternative treatment for acne. |
format | Online Article Text |
id | pubmed-7277416 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72774162020-06-15 In Vitro Antibacterial and Anti-Inflammatory Effects of Novel Insect Fungus Polycephalomyces phaothaiensis Extract and Its Constituents against Propionibacterium acnes Sonyot, Witsanu Lamlertthon, Supaporn Luangsa-ard, Janet Jennifer Mongkolsamrit, Suchada Usuwanthim, Kanchana Ingkaninan, Kornkanok Waranuch, Neti Suphrom, Nungruthai Antibiotics (Basel) Article Propionibacterium acnes plays an important role in the pathophysiology of acne vulgaris, the most common chronic inflammatory skin disease of the pilosebaceous unit. This study was conducted to investigate whether the entomopathogenic fungus Polycephalomyces phaothaiensis components have antibacterial and anti-inflammatory effects against P. acnes that may serve for acne treatment. A chemical study by spectroscopic analysis resulted in the identification of seven known compounds. The anti-P. acnes potency of extracts and test compounds was determined by both agar diffusion and broth dilution methods. The ethyl acetate extract from culture broth along with cordytropolone (1) and stipitalide (2) exhibited strong anti- P. acnes activity while (+)-piliformic acid (3) showed weak inhibitory activity. The anti-inflammatory effect of ethyl acetate extract and 1–3 was then examined by the quantification of pro-inflammatory cytokines IL-1β, IL-6, and TNF-α on heat-killed P. acnes induced cytokine production by THP-1 cells. The result demonstrated that the extract and its constituents (1–3) showed a potent significant effect by inhibiting the P. acnes-induced pro-inflammatory cytokines production in THP-1. Our results suggest for the first time that P. phaothaiensis and its constituents (1 and 2) hold therapeutic value for further studies as a new alternative treatment for acne. MDPI 2020-05-25 /pmc/articles/PMC7277416/ /pubmed/32466146 http://dx.doi.org/10.3390/antibiotics9050274 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sonyot, Witsanu Lamlertthon, Supaporn Luangsa-ard, Janet Jennifer Mongkolsamrit, Suchada Usuwanthim, Kanchana Ingkaninan, Kornkanok Waranuch, Neti Suphrom, Nungruthai In Vitro Antibacterial and Anti-Inflammatory Effects of Novel Insect Fungus Polycephalomyces phaothaiensis Extract and Its Constituents against Propionibacterium acnes |
title | In Vitro Antibacterial and Anti-Inflammatory Effects of Novel Insect Fungus Polycephalomyces phaothaiensis Extract and Its Constituents against Propionibacterium acnes |
title_full | In Vitro Antibacterial and Anti-Inflammatory Effects of Novel Insect Fungus Polycephalomyces phaothaiensis Extract and Its Constituents against Propionibacterium acnes |
title_fullStr | In Vitro Antibacterial and Anti-Inflammatory Effects of Novel Insect Fungus Polycephalomyces phaothaiensis Extract and Its Constituents against Propionibacterium acnes |
title_full_unstemmed | In Vitro Antibacterial and Anti-Inflammatory Effects of Novel Insect Fungus Polycephalomyces phaothaiensis Extract and Its Constituents against Propionibacterium acnes |
title_short | In Vitro Antibacterial and Anti-Inflammatory Effects of Novel Insect Fungus Polycephalomyces phaothaiensis Extract and Its Constituents against Propionibacterium acnes |
title_sort | in vitro antibacterial and anti-inflammatory effects of novel insect fungus polycephalomyces phaothaiensis extract and its constituents against propionibacterium acnes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7277416/ https://www.ncbi.nlm.nih.gov/pubmed/32466146 http://dx.doi.org/10.3390/antibiotics9050274 |
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