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High Primary Antibiotic Resistance of Helicobacter pylori Strains Isolated from Pediatric and Adult Patients in Poland during 2016–2018

Monitoring the antibiotic resistance of H. pylori is an important step in the effective treatment of this bacterium, thus the aim of the present study was to assess the prevalence of antimicrobial resistance of H. pylori strains isolated from pediatric and adult patients with primary infections in 2...

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Autores principales: Krzyżek, Paweł, Pawełka, Dorota, Iwańczak, Barbara, Kempiński, Radosław, Leśniakowski, Konrad, Mégraud, Francis, Łaczmański, Łukasz, Biernat, Monika, Gościniak, Grażyna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7277856/
https://www.ncbi.nlm.nih.gov/pubmed/32370201
http://dx.doi.org/10.3390/antibiotics9050228
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author Krzyżek, Paweł
Pawełka, Dorota
Iwańczak, Barbara
Kempiński, Radosław
Leśniakowski, Konrad
Mégraud, Francis
Łaczmański, Łukasz
Biernat, Monika
Gościniak, Grażyna
author_facet Krzyżek, Paweł
Pawełka, Dorota
Iwańczak, Barbara
Kempiński, Radosław
Leśniakowski, Konrad
Mégraud, Francis
Łaczmański, Łukasz
Biernat, Monika
Gościniak, Grażyna
author_sort Krzyżek, Paweł
collection PubMed
description Monitoring the antibiotic resistance of H. pylori is an important step in the effective treatment of this bacterium, thus the aim of the present study was to assess the prevalence of antimicrobial resistance of H. pylori strains isolated from pediatric and adult patients with primary infections in 2016–2018. Antral biopsies from 334 treatment-naïve patients (126 children and 208 adults) were obtained. A total of 71 clinical H. pylori strains (22 from children and 49 from adults) were isolated and examined for amoxicillin (AMX), clarithromycin (CLR), metronidazole (MTZ), tetracycline (TET), and levofloxacin (LEV) susceptibility. The activity of the antibiotics was measured by E-tests. Strains were considered as resistant to antibiotics with minimum inhibitory concentrations (MICs) equal to ≥0.125 μg/mL (AMX), ≥0.5 μg/mL (CLR), ≥8 μg/mL (MTZ), and ≥1 μg/mL (TET and LEV). The highest prevalence of antibiotic resistance in H. pylori strains was observed for CLR and MTZ, at frequencies of 54.5% and 31.8% vs. 30.6% and 46.9% for children and adults, respectively. A much lower frequency of isolation of resistant strains was demonstrated for LEV and TET, this being 9.1% and 4.5% vs. 18.4% and 4.1% for pediatric and adult patients, respectively. The presence of AMX-resistant strains was not observed. The H. pylori strains isolated from Polish patients with primary infections showed a high level of antibiotic resistance to CLR and MTZ (>30%).
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spelling pubmed-72778562020-06-12 High Primary Antibiotic Resistance of Helicobacter pylori Strains Isolated from Pediatric and Adult Patients in Poland during 2016–2018 Krzyżek, Paweł Pawełka, Dorota Iwańczak, Barbara Kempiński, Radosław Leśniakowski, Konrad Mégraud, Francis Łaczmański, Łukasz Biernat, Monika Gościniak, Grażyna Antibiotics (Basel) Brief Report Monitoring the antibiotic resistance of H. pylori is an important step in the effective treatment of this bacterium, thus the aim of the present study was to assess the prevalence of antimicrobial resistance of H. pylori strains isolated from pediatric and adult patients with primary infections in 2016–2018. Antral biopsies from 334 treatment-naïve patients (126 children and 208 adults) were obtained. A total of 71 clinical H. pylori strains (22 from children and 49 from adults) were isolated and examined for amoxicillin (AMX), clarithromycin (CLR), metronidazole (MTZ), tetracycline (TET), and levofloxacin (LEV) susceptibility. The activity of the antibiotics was measured by E-tests. Strains were considered as resistant to antibiotics with minimum inhibitory concentrations (MICs) equal to ≥0.125 μg/mL (AMX), ≥0.5 μg/mL (CLR), ≥8 μg/mL (MTZ), and ≥1 μg/mL (TET and LEV). The highest prevalence of antibiotic resistance in H. pylori strains was observed for CLR and MTZ, at frequencies of 54.5% and 31.8% vs. 30.6% and 46.9% for children and adults, respectively. A much lower frequency of isolation of resistant strains was demonstrated for LEV and TET, this being 9.1% and 4.5% vs. 18.4% and 4.1% for pediatric and adult patients, respectively. The presence of AMX-resistant strains was not observed. The H. pylori strains isolated from Polish patients with primary infections showed a high level of antibiotic resistance to CLR and MTZ (>30%). MDPI 2020-05-02 /pmc/articles/PMC7277856/ /pubmed/32370201 http://dx.doi.org/10.3390/antibiotics9050228 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Krzyżek, Paweł
Pawełka, Dorota
Iwańczak, Barbara
Kempiński, Radosław
Leśniakowski, Konrad
Mégraud, Francis
Łaczmański, Łukasz
Biernat, Monika
Gościniak, Grażyna
High Primary Antibiotic Resistance of Helicobacter pylori Strains Isolated from Pediatric and Adult Patients in Poland during 2016–2018
title High Primary Antibiotic Resistance of Helicobacter pylori Strains Isolated from Pediatric and Adult Patients in Poland during 2016–2018
title_full High Primary Antibiotic Resistance of Helicobacter pylori Strains Isolated from Pediatric and Adult Patients in Poland during 2016–2018
title_fullStr High Primary Antibiotic Resistance of Helicobacter pylori Strains Isolated from Pediatric and Adult Patients in Poland during 2016–2018
title_full_unstemmed High Primary Antibiotic Resistance of Helicobacter pylori Strains Isolated from Pediatric and Adult Patients in Poland during 2016–2018
title_short High Primary Antibiotic Resistance of Helicobacter pylori Strains Isolated from Pediatric and Adult Patients in Poland during 2016–2018
title_sort high primary antibiotic resistance of helicobacter pylori strains isolated from pediatric and adult patients in poland during 2016–2018
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7277856/
https://www.ncbi.nlm.nih.gov/pubmed/32370201
http://dx.doi.org/10.3390/antibiotics9050228
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