Optimization of the CDC Protocol of Molecular Diagnosis of COVID-19 for Timely Diagnosis

Coronavirus disease 2019 (COVID-19), the current uncontrolled outbreak of infectious disease, has caused significant challenges throughout the world. A reliable rapid diagnostic test for COVID-19 is demanded worldwide. The real-time reverse transcriptase polymerase chain was one of the most quickly...

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Autores principales: Chen, Chao-Ju, Hsieh, Li-Ling, Lin, Shu-Kai, Wang, Chu-Feng, Huang, Yi-Hui, Lin, Shang-Yi, Lu, Po-Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7278012/
https://www.ncbi.nlm.nih.gov/pubmed/32455796
http://dx.doi.org/10.3390/diagnostics10050333
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author Chen, Chao-Ju
Hsieh, Li-Ling
Lin, Shu-Kai
Wang, Chu-Feng
Huang, Yi-Hui
Lin, Shang-Yi
Lu, Po-Liang
author_facet Chen, Chao-Ju
Hsieh, Li-Ling
Lin, Shu-Kai
Wang, Chu-Feng
Huang, Yi-Hui
Lin, Shang-Yi
Lu, Po-Liang
author_sort Chen, Chao-Ju
collection PubMed
description Coronavirus disease 2019 (COVID-19), the current uncontrolled outbreak of infectious disease, has caused significant challenges throughout the world. A reliable rapid diagnostic test for COVID-19 is demanded worldwide. The real-time reverse transcriptase polymerase chain was one of the most quickly established methods in the novel viral pandemic and was considered as the gold standard for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this report, we illustrate our experience of applying a protocol from the Taiwan CDC and achieving assay optimization in the immediate circumstances to meet the urgent medical and public health needs.
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spelling pubmed-72780122020-06-12 Optimization of the CDC Protocol of Molecular Diagnosis of COVID-19 for Timely Diagnosis Chen, Chao-Ju Hsieh, Li-Ling Lin, Shu-Kai Wang, Chu-Feng Huang, Yi-Hui Lin, Shang-Yi Lu, Po-Liang Diagnostics (Basel) Communication Coronavirus disease 2019 (COVID-19), the current uncontrolled outbreak of infectious disease, has caused significant challenges throughout the world. A reliable rapid diagnostic test for COVID-19 is demanded worldwide. The real-time reverse transcriptase polymerase chain was one of the most quickly established methods in the novel viral pandemic and was considered as the gold standard for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this report, we illustrate our experience of applying a protocol from the Taiwan CDC and achieving assay optimization in the immediate circumstances to meet the urgent medical and public health needs. MDPI 2020-05-21 /pmc/articles/PMC7278012/ /pubmed/32455796 http://dx.doi.org/10.3390/diagnostics10050333 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Chen, Chao-Ju
Hsieh, Li-Ling
Lin, Shu-Kai
Wang, Chu-Feng
Huang, Yi-Hui
Lin, Shang-Yi
Lu, Po-Liang
Optimization of the CDC Protocol of Molecular Diagnosis of COVID-19 for Timely Diagnosis
title Optimization of the CDC Protocol of Molecular Diagnosis of COVID-19 for Timely Diagnosis
title_full Optimization of the CDC Protocol of Molecular Diagnosis of COVID-19 for Timely Diagnosis
title_fullStr Optimization of the CDC Protocol of Molecular Diagnosis of COVID-19 for Timely Diagnosis
title_full_unstemmed Optimization of the CDC Protocol of Molecular Diagnosis of COVID-19 for Timely Diagnosis
title_short Optimization of the CDC Protocol of Molecular Diagnosis of COVID-19 for Timely Diagnosis
title_sort optimization of the cdc protocol of molecular diagnosis of covid-19 for timely diagnosis
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7278012/
https://www.ncbi.nlm.nih.gov/pubmed/32455796
http://dx.doi.org/10.3390/diagnostics10050333
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