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Direct-seq: programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen

CRISPR-based genome perturbation provides a new avenue to conveniently change DNA sequences, transcription, and epigenetic modifications in genetic screens. However, it remains challenging to assay the complex molecular readouts after perturbation at high resolution and at scale. By introducing an A...

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Detalles Bibliográficos
Autores principales:	Song, Qingkai, Ni, Ke, Liu, Min, Li, Yini, Wang, Lixia, Wang, Yingying, Liu, Yingzheng, Yu, Zhenxing, Qi, Yinyao, Lu, Zhike, Ma, Lijia
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2020
Materias:	Method
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7278172/ https://www.ncbi.nlm.nih.gov/pubmed/32513233 http://dx.doi.org/10.1186/s13059-020-02044-w

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