Toxicity of Carbon, Silicon, and Metal-Based Nanoparticles to the Hemocytes of Three Marine Bivalves

SIMPLE SUMMARY: The growing nanotechnology industry disposes of a variety of nanoparticles with different physiochemical properties in everyday life. However, the dependence of the safety and toxicity of nanoparticles on their physicochemical properties remains unclear. Bivalve molluscs represent an efficient model for the investigation of nanoparticle toxicity owing to their filtrating ability and feeding on particles suspended in the water. Moreover, the blood cells of bivalve molluscs, the hemocytes, have been suggested as a good analog test-object to mammalian immune cells, phagocytes. In this study, we used hemocytes of three marine bivalve species, namely, Crenomytilus grayanus, Modiolus modiolus, and Arca boucardi, to evaluate and compare the toxic effects of 10 different types of nanoparticles. We gave short-term exposure of the nanoparticles to the hemocytes and registered viability and changes in their cell membrane polarization by employing flow cytometry. Metal-based nanoparticles were the most toxic to the cells of all three tested bivalve mollusc species. However, the sensitivity to different nanoparticle types varied between species. Moreover, the registered cell membrane depolarization indicated an early toxic response and raised concern that chronic long-term exposure of nanoparticles (even if they were previously declared as safe) is a serious threat for aquatic organisms. ABSTRACT: Nanoparticles (NPs) have broad applications in medicine, cosmetics, optics, catalysis, environmental purification, and other areas nowadays. With increasing annual production of NPs, the risks of their harmful influence on the environment and human health are also increasing. Currently, our knowledge about the mechanisms of the interaction between NPs and living organisms is limited. The marine species and their habitat environment are under continuous stress owing to the anthropogenic activities, which result in the release of NPs in the aquatic environment. We used a bioassay model with hemocytes of three bivalve mollusc species, namely, Crenomytilus grayanus, Modiolus modiolus, and Arca boucardi, to evaluate the toxicity of 10 different types of NPs. Specifically, we compared the cytotoxic effects and cell-membrane polarization changes in the hemocytes exposed to carbon nanotubes, carbon nanofibers, silicon nanotubes, cadmium and zinc sulfides, Au-NPs, and TiO(2) NPs. Viability and the changes in hemocyte membrane polarization were measured by the flow cytometry method. The highest aquatic toxicity was registered for metal-based NPs, which caused cytotoxicity to the hemocytes of all the studied bivalve species. Our results also highlighted different sensitivities of the used tested mollusc species to specific NPs.