Combination of Bacillus licheniformis and Salinomycin: Effect on the Growth Performance and GIT Microbial Populations of Broiler Chickens

SIMPLE SUMMARY: The beneficial effects of Bacillus spp. probiotic preparations used for poultry are well-documented and characterized by growth performance improvement and positive modulation of gastrointestinal tract (GIT) microbiota. Moreover, the favorable influence of salinomycin has been frequently studied as an ionophore coccidiostat, as well as an antimicrobial agent. However, limited data are available in terms of the parallel usage of both Bacillus licheniformis DSM 28710 and salinomycin in poultry diets. From a practical point of view, evaluating the potential interactions between this species and agent is crucial to assess their parallel usage, and the current study confirmed the positive effect of their mixture on the modulation of pH value in the crop and ceca, as well as the GIT microbiota, especially in the jejunum and ceca. Additionally, the results obtained in this study show positive effects of B. licheniformis on the growth performance, as well as the influence of both experimental factors used separately in the case of GIT microbiota modulations. ABSTRACT: The aim of the study was to investigate the effect of Bacillus licheniformis and salinomycin supplementation in broiler diets as individual factors or in combination on the growth performance, GIT morphometry, and microbiota populations. Four hundred one-day-old Ross 308 chicks were randomly distributed to four dietary treatments (10 replicates, 10 birds each). The following treatments were applied: NC—no additives; NC + SAL—salinomycin addition (60 mg/kg diet), NC + PRO—B. licheniformis DSM 28710 preparation (1.6 × 10(9) CFU/kg; 500 mg/kg diet), and NC + SAL + PRO—combination of salinomycin and B. licheniformis. Probiotic administration resulted in improvement (p < 0.05) of the performance parameters, including body weight gain (1–10 d, and 11–22 d) and feed conversion ratio (11–22 d, 1–36 d). An interaction (p < 0.05) between experimental factors was observed in terms of lower pH values in the crop (tendency, p = 0.053) and ceca. Both factors lowered the alpha diversity and Enterobacteriaceae and promoted Bacillaceae communities in the jejunum (p < 0.05). Interactions were also observed in terms of reducing Clostridiaceae in the ceca. In conclusion, the combined use of B. licheniformis and salinomycin in broilers’ diets had beneficial effects.