Comparative Semen Microbiota Composition of a Stallion in a Taylorella equigenitalis Carrier and Non-Carrier State

SIMPLE SUMMARY: Contagious equine metritis carriers have become a new cause of concern in horse stud farms. Their detection can result in significant financial loss and force owners to have their animals undergo antibiotic treatment. Current research has not been able to satisfactorily explain the appearance of carriers in agent-free farms. Studies made on microbial flora have given new insights into the diagnosis and treatment of different issues in animal systems. Next-generation sequencing (NGS) is a powerful tool that can draw an accurate picture of microbial flora. Therefore, the aim of this study was to compare the seminal bacterial composition of one stallion before and after being diagnosed with Taylorella equigenitalis using NGS. Our results show that the microbial seminal flora visibly changed between the samples analyzed. Corynebacteriaceae, an opportunistic bacterial family, was more common in the infected sample. However, Porphyromonadaceae, a natural component in several tissues, was more abundant in the negative sample. Despite the constraints of a single-case study, these findings can open the door to new therapeutic tools, as flora transplants. Similarly, seminal flora analysis may foresee microbial shifts, letting practitioners take preventive actions before a potential outbreak. Furthermore, these actions would have the extra benefit of reducing the administration of antibiotics to treat an infection. ABSTRACT: Contagious equine metritis is receiving renewed attention due to the continuous detection of carriers in apparent agent-free farms. Interactions of Taylorella with the seminal microflora may be the plausible cause behind these spontaneous changes of the carrier state. Accordingly, the aim of this study was to compare the differences in the seminal microbiome composition of one stallion in the contagious equine metritis carrier state and non-carrier state. Samples were cryopreserved after their extraction. Cell disruption was performed by high-speed homogenization in grinding media. Bacterial families were identified via V3 amplification of the 16S rRNA gene and Ion Torrent sequencing. Only bacterial families with relative abundance above 5% were taken into consideration. The positive sample contained a strong dominance of Corynebacteriaceae (37.75%) and Peptoniphilaceae (28.56%). In the negative sample, the Porphyromonadaceae (20.51%), Bacteroidaceae (19.25%) and Peptoniphilaceae (18.57%) families prevailed. In conclusion, the microbiome seminal composition varies when an individual carries Taylorella from when it is free of it. The wider differences were found in the Corynebacteriaceae, Porphyromonadaceae and Bacteroidaceae families. Due to the limitations of a single-case analysis, further studies are needed for a better understanding of the stallion seminal microflora interactions.