Aquaporin 1 (AQP1) Expression in Healthy Dog Tears

SIMPLE SUMMARY: The characterisation of tear proteins is very important for scientists and clinicians, as it enhances their understanding of ocular physiological phenomena that sometimes evolve into diseases. Recently, ophthalmic research has been focused on aquaporins (AQPs), a family of water channel proteins that are largely ubiquitous in body tissues and are known for their role in water and small solute transport across cell membranes. Based on AQPs’ presumable role in the eye, the aim of the present study was to investigate the expression of aquaporin-1 (AQP1) by Western blot analysis in canine eye tears. To this end, we collected tears from both eyes of 15 healthy dogs by employing two tear collection methods: Schirmer tear strips (STS) and ophthalmic sponges (OS). Moreover, ocular parameters such as Schirmer tear test 1 (STT 1), intraocular pressure (IOP), and tear film break up time (BUT) were measured, and fluorescein and lissamine green staining were performed to uncover possible correlations among the aforementioned parameters. Our results showed that the expression of AQP1 in tears collected by both methods and expressed as multiple bands (measured by densitometry) was higher for the tears collected by OS than for those collected by STS. This work forms the basis of future studies aiming to understand and establish the involvement of AQPs in the production and secretion of tears. ABSTRACT: Aquaporins (AQPs) are a family of thirteen membrane proteins that play an essential role in the transport of fluids across the cell plasma membrane. Recently, the expression of AQPs in different ocular tissues and their involvement in the pathophysiology of eye diseases, have garnered attention. Considering that literature on AQP expression in the lacrimal glands and their secretion is scarce, we aimed to characterise AQP1 expression in the tears of healthy dogs using two tear collection methods (Schirmer tear strips (STS) and ophthalmic sponges (OS)). Fifteen healthy dogs, free of ophthalmic diseases, were included in the study. Tear collection was performed by using STS in one eye and OS in the other. After the extraction of proteins from the tears, the expression of AQP1 was analysed by Western blotting. AQP1 was expressed as a band of 28 kDa. In addition, differences were observed in the expression of AQP1 and in the correlation between tear volume and protein concentration, in tears collected by the two different methods. Our results suggest that AQP1 has a specific role in tear secretion; further research is required to assess its particular role in the function of the ocular surface in eye physiology and pathology.