Cargando…

The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation

The structural integrity, elasticity, and fluidity of lipid membranes are critical for cellular activities such as communication between cells, exocytosis, and endocytosis. Unsaturated lipids, the main components of biological membranes, are particularly susceptible to the oxidative attack of reacti...

Descripción completa

Detalles Bibliográficos
Autores principales: Sadžak, Anja, Mravljak, Janez, Maltar-Strmečki, Nadica, Arsov, Zoran, Baranović, Goran, Erceg, Ina, Kriechbaum, Manfred, Strasser, Vida, Přibyl, Jan, Šegota, Suzana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7278707/
https://www.ncbi.nlm.nih.gov/pubmed/32429305
http://dx.doi.org/10.3390/antiox9050430
_version_ 1783543393632124928
author Sadžak, Anja
Mravljak, Janez
Maltar-Strmečki, Nadica
Arsov, Zoran
Baranović, Goran
Erceg, Ina
Kriechbaum, Manfred
Strasser, Vida
Přibyl, Jan
Šegota, Suzana
author_facet Sadžak, Anja
Mravljak, Janez
Maltar-Strmečki, Nadica
Arsov, Zoran
Baranović, Goran
Erceg, Ina
Kriechbaum, Manfred
Strasser, Vida
Přibyl, Jan
Šegota, Suzana
author_sort Sadžak, Anja
collection PubMed
description The structural integrity, elasticity, and fluidity of lipid membranes are critical for cellular activities such as communication between cells, exocytosis, and endocytosis. Unsaturated lipids, the main components of biological membranes, are particularly susceptible to the oxidative attack of reactive oxygen species. The peroxidation of unsaturated lipids, in our case 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), induces the structural reorganization of the membrane. We have employed a multi-technique approach to analyze typical properties of lipid bilayers, i.e., roughness, thickness, elasticity, and fluidity. We compared the alteration of the membrane properties upon initiated lipid peroxidation and examined the ability of flavonols, namely quercetin (QUE), myricetin (MCE), and myricitrin (MCI) at different molar fractions, to inhibit this change. Using Mass Spectrometry (MS) and Fourier Transform Infrared Spectroscopy (FTIR), we identified various carbonyl products and examined the extent of the reaction. From Atomic Force Microscopy (AFM), Force Spectroscopy (FS), Small Angle X-Ray Scattering (SAXS), and Electron Paramagnetic Resonance (EPR) experiments, we concluded that the membranes with inserted flavonols exhibit resistance against the structural changes induced by the oxidative attack, which is a finding with multiple biological implications. Our approach reveals the interplay between the flavonol molecular structure and the crucial membrane properties under oxidative attack and provides insight into the pathophysiology of cellular oxidative injury.
format Online
Article
Text
id pubmed-7278707
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-72787072020-06-12 The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation Sadžak, Anja Mravljak, Janez Maltar-Strmečki, Nadica Arsov, Zoran Baranović, Goran Erceg, Ina Kriechbaum, Manfred Strasser, Vida Přibyl, Jan Šegota, Suzana Antioxidants (Basel) Article The structural integrity, elasticity, and fluidity of lipid membranes are critical for cellular activities such as communication between cells, exocytosis, and endocytosis. Unsaturated lipids, the main components of biological membranes, are particularly susceptible to the oxidative attack of reactive oxygen species. The peroxidation of unsaturated lipids, in our case 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), induces the structural reorganization of the membrane. We have employed a multi-technique approach to analyze typical properties of lipid bilayers, i.e., roughness, thickness, elasticity, and fluidity. We compared the alteration of the membrane properties upon initiated lipid peroxidation and examined the ability of flavonols, namely quercetin (QUE), myricetin (MCE), and myricitrin (MCI) at different molar fractions, to inhibit this change. Using Mass Spectrometry (MS) and Fourier Transform Infrared Spectroscopy (FTIR), we identified various carbonyl products and examined the extent of the reaction. From Atomic Force Microscopy (AFM), Force Spectroscopy (FS), Small Angle X-Ray Scattering (SAXS), and Electron Paramagnetic Resonance (EPR) experiments, we concluded that the membranes with inserted flavonols exhibit resistance against the structural changes induced by the oxidative attack, which is a finding with multiple biological implications. Our approach reveals the interplay between the flavonol molecular structure and the crucial membrane properties under oxidative attack and provides insight into the pathophysiology of cellular oxidative injury. MDPI 2020-05-15 /pmc/articles/PMC7278707/ /pubmed/32429305 http://dx.doi.org/10.3390/antiox9050430 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sadžak, Anja
Mravljak, Janez
Maltar-Strmečki, Nadica
Arsov, Zoran
Baranović, Goran
Erceg, Ina
Kriechbaum, Manfred
Strasser, Vida
Přibyl, Jan
Šegota, Suzana
The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation
title The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation
title_full The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation
title_fullStr The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation
title_full_unstemmed The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation
title_short The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation
title_sort structural integrity of the model lipid membrane during induced lipid peroxidation: the role of flavonols in the inhibition of lipid peroxidation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7278707/
https://www.ncbi.nlm.nih.gov/pubmed/32429305
http://dx.doi.org/10.3390/antiox9050430
work_keys_str_mv AT sadzakanja thestructuralintegrityofthemodellipidmembraneduringinducedlipidperoxidationtheroleofflavonolsintheinhibitionoflipidperoxidation
AT mravljakjanez thestructuralintegrityofthemodellipidmembraneduringinducedlipidperoxidationtheroleofflavonolsintheinhibitionoflipidperoxidation
AT maltarstrmeckinadica thestructuralintegrityofthemodellipidmembraneduringinducedlipidperoxidationtheroleofflavonolsintheinhibitionoflipidperoxidation
AT arsovzoran thestructuralintegrityofthemodellipidmembraneduringinducedlipidperoxidationtheroleofflavonolsintheinhibitionoflipidperoxidation
AT baranovicgoran thestructuralintegrityofthemodellipidmembraneduringinducedlipidperoxidationtheroleofflavonolsintheinhibitionoflipidperoxidation
AT ercegina thestructuralintegrityofthemodellipidmembraneduringinducedlipidperoxidationtheroleofflavonolsintheinhibitionoflipidperoxidation
AT kriechbaummanfred thestructuralintegrityofthemodellipidmembraneduringinducedlipidperoxidationtheroleofflavonolsintheinhibitionoflipidperoxidation
AT strasservida thestructuralintegrityofthemodellipidmembraneduringinducedlipidperoxidationtheroleofflavonolsintheinhibitionoflipidperoxidation
AT pribyljan thestructuralintegrityofthemodellipidmembraneduringinducedlipidperoxidationtheroleofflavonolsintheinhibitionoflipidperoxidation
AT segotasuzana thestructuralintegrityofthemodellipidmembraneduringinducedlipidperoxidationtheroleofflavonolsintheinhibitionoflipidperoxidation
AT sadzakanja structuralintegrityofthemodellipidmembraneduringinducedlipidperoxidationtheroleofflavonolsintheinhibitionoflipidperoxidation
AT mravljakjanez structuralintegrityofthemodellipidmembraneduringinducedlipidperoxidationtheroleofflavonolsintheinhibitionoflipidperoxidation
AT maltarstrmeckinadica structuralintegrityofthemodellipidmembraneduringinducedlipidperoxidationtheroleofflavonolsintheinhibitionoflipidperoxidation
AT arsovzoran structuralintegrityofthemodellipidmembraneduringinducedlipidperoxidationtheroleofflavonolsintheinhibitionoflipidperoxidation
AT baranovicgoran structuralintegrityofthemodellipidmembraneduringinducedlipidperoxidationtheroleofflavonolsintheinhibitionoflipidperoxidation
AT ercegina structuralintegrityofthemodellipidmembraneduringinducedlipidperoxidationtheroleofflavonolsintheinhibitionoflipidperoxidation
AT kriechbaummanfred structuralintegrityofthemodellipidmembraneduringinducedlipidperoxidationtheroleofflavonolsintheinhibitionoflipidperoxidation
AT strasservida structuralintegrityofthemodellipidmembraneduringinducedlipidperoxidationtheroleofflavonolsintheinhibitionoflipidperoxidation
AT pribyljan structuralintegrityofthemodellipidmembraneduringinducedlipidperoxidationtheroleofflavonolsintheinhibitionoflipidperoxidation
AT segotasuzana structuralintegrityofthemodellipidmembraneduringinducedlipidperoxidationtheroleofflavonolsintheinhibitionoflipidperoxidation