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Season of Birth Impacts the Neonatal Nasopharyngeal Microbiota

Objective: Pathogenic airway bacteria colonizing the neonatal airway increase the risk of childhood asthma, but little is known about the determinants of the establishment and dynamics of the airway microbiota in early life. We studied associations between perinatal risk factors and bacterial richne...

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Autores principales: Schoos, Ann-Marie Malby, Kragh, Marie, Ahrens, Peter, Kuhn, Katrin Gaardbo, Rasmussen, Morten Arendt, Chawes, Bo Lund, Jensen, Jørgen Skov, Brix, Susanne, Bisgaard, Hans, Stokholm, Jakob
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7278723/
https://www.ncbi.nlm.nih.gov/pubmed/32403236
http://dx.doi.org/10.3390/children7050045
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author Schoos, Ann-Marie Malby
Kragh, Marie
Ahrens, Peter
Kuhn, Katrin Gaardbo
Rasmussen, Morten Arendt
Chawes, Bo Lund
Jensen, Jørgen Skov
Brix, Susanne
Bisgaard, Hans
Stokholm, Jakob
author_facet Schoos, Ann-Marie Malby
Kragh, Marie
Ahrens, Peter
Kuhn, Katrin Gaardbo
Rasmussen, Morten Arendt
Chawes, Bo Lund
Jensen, Jørgen Skov
Brix, Susanne
Bisgaard, Hans
Stokholm, Jakob
author_sort Schoos, Ann-Marie Malby
collection PubMed
description Objective: Pathogenic airway bacteria colonizing the neonatal airway increase the risk of childhood asthma, but little is known about the determinants of the establishment and dynamics of the airway microbiota in early life. We studied associations between perinatal risk factors and bacterial richness of the commensal milieu in the neonatal respiratory tract. Methods: Three hundred and twenty-eight children from the Copenhagen Prospective Studies on Asthma in the Childhood2000 (COPSAC2000) at-risk birth cohort were included in this study. The bacterial richness in each of the nasopharynxes of the 1-month old, asymptomatic neonates was analyzed by use of a culture-independent technique (T-RFLP). Information on perinatal risk factors included predisposition to asthma, allergy and eczema; social status of family; maternal exposures during pregnancy; mode of delivery; and postnatal exposures. The risk factor analysis was done by conventional statistics and partial least square discriminant analysis (PLSDA). Results: The nasopharyngeal bacterial community at 1-month displayed an average of 35 (IQR: 14–55, range 1–161) phylogenetically different bacteria groups. Season of birth was associated with nasopharyngeal bacterial richness at 1-month of age with a higher bacterial richness (p = 0.003) and more abundant specific bacterial profiles representing Gram-negative alpha-proteobacteria and Gram-positive Bacilli in the nasopharynx of summer-born children. Conclusion: Early postnatal bacterial colonization of the upper airways is significantly affected by birth season, emphasizing a future focus on the seasonality aspect in modelling the impact of early dynamic changes in airway bacterial communities in relation to later disease development.
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spelling pubmed-72787232020-06-12 Season of Birth Impacts the Neonatal Nasopharyngeal Microbiota Schoos, Ann-Marie Malby Kragh, Marie Ahrens, Peter Kuhn, Katrin Gaardbo Rasmussen, Morten Arendt Chawes, Bo Lund Jensen, Jørgen Skov Brix, Susanne Bisgaard, Hans Stokholm, Jakob Children (Basel) Article Objective: Pathogenic airway bacteria colonizing the neonatal airway increase the risk of childhood asthma, but little is known about the determinants of the establishment and dynamics of the airway microbiota in early life. We studied associations between perinatal risk factors and bacterial richness of the commensal milieu in the neonatal respiratory tract. Methods: Three hundred and twenty-eight children from the Copenhagen Prospective Studies on Asthma in the Childhood2000 (COPSAC2000) at-risk birth cohort were included in this study. The bacterial richness in each of the nasopharynxes of the 1-month old, asymptomatic neonates was analyzed by use of a culture-independent technique (T-RFLP). Information on perinatal risk factors included predisposition to asthma, allergy and eczema; social status of family; maternal exposures during pregnancy; mode of delivery; and postnatal exposures. The risk factor analysis was done by conventional statistics and partial least square discriminant analysis (PLSDA). Results: The nasopharyngeal bacterial community at 1-month displayed an average of 35 (IQR: 14–55, range 1–161) phylogenetically different bacteria groups. Season of birth was associated with nasopharyngeal bacterial richness at 1-month of age with a higher bacterial richness (p = 0.003) and more abundant specific bacterial profiles representing Gram-negative alpha-proteobacteria and Gram-positive Bacilli in the nasopharynx of summer-born children. Conclusion: Early postnatal bacterial colonization of the upper airways is significantly affected by birth season, emphasizing a future focus on the seasonality aspect in modelling the impact of early dynamic changes in airway bacterial communities in relation to later disease development. MDPI 2020-05-11 /pmc/articles/PMC7278723/ /pubmed/32403236 http://dx.doi.org/10.3390/children7050045 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Schoos, Ann-Marie Malby
Kragh, Marie
Ahrens, Peter
Kuhn, Katrin Gaardbo
Rasmussen, Morten Arendt
Chawes, Bo Lund
Jensen, Jørgen Skov
Brix, Susanne
Bisgaard, Hans
Stokholm, Jakob
Season of Birth Impacts the Neonatal Nasopharyngeal Microbiota
title Season of Birth Impacts the Neonatal Nasopharyngeal Microbiota
title_full Season of Birth Impacts the Neonatal Nasopharyngeal Microbiota
title_fullStr Season of Birth Impacts the Neonatal Nasopharyngeal Microbiota
title_full_unstemmed Season of Birth Impacts the Neonatal Nasopharyngeal Microbiota
title_short Season of Birth Impacts the Neonatal Nasopharyngeal Microbiota
title_sort season of birth impacts the neonatal nasopharyngeal microbiota
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7278723/
https://www.ncbi.nlm.nih.gov/pubmed/32403236
http://dx.doi.org/10.3390/children7050045
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