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Light Microscopy as a Tool to Evaluate the Functionality of Starch in Food
Light microscopy (LM) is commonly used in the study of biological materials to determine the morphology of cells and tissues. The potential of this technique for studying the structure of food products is also recognized but less known. Especially rare are information regarding LM studies of the sup...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7278772/ https://www.ncbi.nlm.nih.gov/pubmed/32455966 http://dx.doi.org/10.3390/foods9050670 |
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author | Błaszczak, Wioletta Lewandowicz, Grażyna |
author_facet | Błaszczak, Wioletta Lewandowicz, Grażyna |
author_sort | Błaszczak, Wioletta |
collection | PubMed |
description | Light microscopy (LM) is commonly used in the study of biological materials to determine the morphology of cells and tissues. The potential of this technique for studying the structure of food products is also recognized but less known. Especially rare are information regarding LM studies of the supramolecular structure of starch. The aim of the work was to fill this gap by providing data on the possibilities for application of LM in starch studies. It was shown that in spite of an enormous progress in the development of microscopic techniques, including both increase of resolution and improvement of image analysis methods, light microscopy still has a huge potential for starch studies. The advantage of LM over other microscopic techniques is the possibility of differentiating between amylose and amylopectin by iodine staining. That makes LM especially useful in the analysis of the process of gelatinization of starch, the extent of molecular dispersion of its macromolecules, and the changes in its structure caused by modification. Moreover, it can be particularly useful for studying the changes in the supramolecular structure of starch in a food product matrix, providing more information than scanning electron microscopy (SEM)–the most common technique used for these purposes. |
format | Online Article Text |
id | pubmed-7278772 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72787722020-06-12 Light Microscopy as a Tool to Evaluate the Functionality of Starch in Food Błaszczak, Wioletta Lewandowicz, Grażyna Foods Article Light microscopy (LM) is commonly used in the study of biological materials to determine the morphology of cells and tissues. The potential of this technique for studying the structure of food products is also recognized but less known. Especially rare are information regarding LM studies of the supramolecular structure of starch. The aim of the work was to fill this gap by providing data on the possibilities for application of LM in starch studies. It was shown that in spite of an enormous progress in the development of microscopic techniques, including both increase of resolution and improvement of image analysis methods, light microscopy still has a huge potential for starch studies. The advantage of LM over other microscopic techniques is the possibility of differentiating between amylose and amylopectin by iodine staining. That makes LM especially useful in the analysis of the process of gelatinization of starch, the extent of molecular dispersion of its macromolecules, and the changes in its structure caused by modification. Moreover, it can be particularly useful for studying the changes in the supramolecular structure of starch in a food product matrix, providing more information than scanning electron microscopy (SEM)–the most common technique used for these purposes. MDPI 2020-05-22 /pmc/articles/PMC7278772/ /pubmed/32455966 http://dx.doi.org/10.3390/foods9050670 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Błaszczak, Wioletta Lewandowicz, Grażyna Light Microscopy as a Tool to Evaluate the Functionality of Starch in Food |
title | Light Microscopy as a Tool to Evaluate the Functionality of Starch in Food |
title_full | Light Microscopy as a Tool to Evaluate the Functionality of Starch in Food |
title_fullStr | Light Microscopy as a Tool to Evaluate the Functionality of Starch in Food |
title_full_unstemmed | Light Microscopy as a Tool to Evaluate the Functionality of Starch in Food |
title_short | Light Microscopy as a Tool to Evaluate the Functionality of Starch in Food |
title_sort | light microscopy as a tool to evaluate the functionality of starch in food |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7278772/ https://www.ncbi.nlm.nih.gov/pubmed/32455966 http://dx.doi.org/10.3390/foods9050670 |
work_keys_str_mv | AT błaszczakwioletta lightmicroscopyasatooltoevaluatethefunctionalityofstarchinfood AT lewandowiczgrazyna lightmicroscopyasatooltoevaluatethefunctionalityofstarchinfood |