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Keratin Biomembranes as a Model for Studying Onychomycosis

Difficulties in obtaining human nails that are large enough for examining the penetration of drug formulations led us to produce keratin films regenerated from human hair. We assume that these films can simulate human nail plates in drug penetration and permeation tests and can serve as a biological...

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Detalles Bibliográficos
Autores principales: Valkov, Anton, Zinigrad, Michael, Sobolev, Alexander, Nisnevitch, Marina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7278978/
https://www.ncbi.nlm.nih.gov/pubmed/32429167
http://dx.doi.org/10.3390/ijms21103512
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author Valkov, Anton
Zinigrad, Michael
Sobolev, Alexander
Nisnevitch, Marina
author_facet Valkov, Anton
Zinigrad, Michael
Sobolev, Alexander
Nisnevitch, Marina
author_sort Valkov, Anton
collection PubMed
description Difficulties in obtaining human nails that are large enough for examining the penetration of drug formulations led us to produce keratin films regenerated from human hair. We assume that these films can simulate human nail plates in drug penetration and permeation tests and can serve as a biological model for studying onychomycosis. The films were formed from keratin extracted from human hair using dithiothreitol, urea and thiourea. The obtained keratin extract was dispensed into Teflon rings and dried at 40 °C and then cured at 110 °C. The structure, surface morphology, chemical characterization and thermal stability of the films were characterized and were compared to those of human nail, hair and bovine hoof samples using SDS-electrophoresis, scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA). The structure of the obtained films was found to be closer to human nails than to hair or bovine hooves. The keratin films were infected with Trichophyton rubrum and were proven to be appropriate for serving as a model for studying onychomycosis.
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spelling pubmed-72789782020-06-15 Keratin Biomembranes as a Model for Studying Onychomycosis Valkov, Anton Zinigrad, Michael Sobolev, Alexander Nisnevitch, Marina Int J Mol Sci Article Difficulties in obtaining human nails that are large enough for examining the penetration of drug formulations led us to produce keratin films regenerated from human hair. We assume that these films can simulate human nail plates in drug penetration and permeation tests and can serve as a biological model for studying onychomycosis. The films were formed from keratin extracted from human hair using dithiothreitol, urea and thiourea. The obtained keratin extract was dispensed into Teflon rings and dried at 40 °C and then cured at 110 °C. The structure, surface morphology, chemical characterization and thermal stability of the films were characterized and were compared to those of human nail, hair and bovine hoof samples using SDS-electrophoresis, scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA). The structure of the obtained films was found to be closer to human nails than to hair or bovine hooves. The keratin films were infected with Trichophyton rubrum and were proven to be appropriate for serving as a model for studying onychomycosis. MDPI 2020-05-15 /pmc/articles/PMC7278978/ /pubmed/32429167 http://dx.doi.org/10.3390/ijms21103512 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Valkov, Anton
Zinigrad, Michael
Sobolev, Alexander
Nisnevitch, Marina
Keratin Biomembranes as a Model for Studying Onychomycosis
title Keratin Biomembranes as a Model for Studying Onychomycosis
title_full Keratin Biomembranes as a Model for Studying Onychomycosis
title_fullStr Keratin Biomembranes as a Model for Studying Onychomycosis
title_full_unstemmed Keratin Biomembranes as a Model for Studying Onychomycosis
title_short Keratin Biomembranes as a Model for Studying Onychomycosis
title_sort keratin biomembranes as a model for studying onychomycosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7278978/
https://www.ncbi.nlm.nih.gov/pubmed/32429167
http://dx.doi.org/10.3390/ijms21103512
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AT nisnevitchmarina keratinbiomembranesasamodelforstudyingonychomycosis