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Keratin Biomembranes as a Model for Studying Onychomycosis
Difficulties in obtaining human nails that are large enough for examining the penetration of drug formulations led us to produce keratin films regenerated from human hair. We assume that these films can simulate human nail plates in drug penetration and permeation tests and can serve as a biological...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7278978/ https://www.ncbi.nlm.nih.gov/pubmed/32429167 http://dx.doi.org/10.3390/ijms21103512 |
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author | Valkov, Anton Zinigrad, Michael Sobolev, Alexander Nisnevitch, Marina |
author_facet | Valkov, Anton Zinigrad, Michael Sobolev, Alexander Nisnevitch, Marina |
author_sort | Valkov, Anton |
collection | PubMed |
description | Difficulties in obtaining human nails that are large enough for examining the penetration of drug formulations led us to produce keratin films regenerated from human hair. We assume that these films can simulate human nail plates in drug penetration and permeation tests and can serve as a biological model for studying onychomycosis. The films were formed from keratin extracted from human hair using dithiothreitol, urea and thiourea. The obtained keratin extract was dispensed into Teflon rings and dried at 40 °C and then cured at 110 °C. The structure, surface morphology, chemical characterization and thermal stability of the films were characterized and were compared to those of human nail, hair and bovine hoof samples using SDS-electrophoresis, scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA). The structure of the obtained films was found to be closer to human nails than to hair or bovine hooves. The keratin films were infected with Trichophyton rubrum and were proven to be appropriate for serving as a model for studying onychomycosis. |
format | Online Article Text |
id | pubmed-7278978 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72789782020-06-15 Keratin Biomembranes as a Model for Studying Onychomycosis Valkov, Anton Zinigrad, Michael Sobolev, Alexander Nisnevitch, Marina Int J Mol Sci Article Difficulties in obtaining human nails that are large enough for examining the penetration of drug formulations led us to produce keratin films regenerated from human hair. We assume that these films can simulate human nail plates in drug penetration and permeation tests and can serve as a biological model for studying onychomycosis. The films were formed from keratin extracted from human hair using dithiothreitol, urea and thiourea. The obtained keratin extract was dispensed into Teflon rings and dried at 40 °C and then cured at 110 °C. The structure, surface morphology, chemical characterization and thermal stability of the films were characterized and were compared to those of human nail, hair and bovine hoof samples using SDS-electrophoresis, scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA). The structure of the obtained films was found to be closer to human nails than to hair or bovine hooves. The keratin films were infected with Trichophyton rubrum and were proven to be appropriate for serving as a model for studying onychomycosis. MDPI 2020-05-15 /pmc/articles/PMC7278978/ /pubmed/32429167 http://dx.doi.org/10.3390/ijms21103512 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Valkov, Anton Zinigrad, Michael Sobolev, Alexander Nisnevitch, Marina Keratin Biomembranes as a Model for Studying Onychomycosis |
title | Keratin Biomembranes as a Model for Studying Onychomycosis |
title_full | Keratin Biomembranes as a Model for Studying Onychomycosis |
title_fullStr | Keratin Biomembranes as a Model for Studying Onychomycosis |
title_full_unstemmed | Keratin Biomembranes as a Model for Studying Onychomycosis |
title_short | Keratin Biomembranes as a Model for Studying Onychomycosis |
title_sort | keratin biomembranes as a model for studying onychomycosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7278978/ https://www.ncbi.nlm.nih.gov/pubmed/32429167 http://dx.doi.org/10.3390/ijms21103512 |
work_keys_str_mv | AT valkovanton keratinbiomembranesasamodelforstudyingonychomycosis AT zinigradmichael keratinbiomembranesasamodelforstudyingonychomycosis AT sobolevalexander keratinbiomembranesasamodelforstudyingonychomycosis AT nisnevitchmarina keratinbiomembranesasamodelforstudyingonychomycosis |