Cargando…

A Novel 3D Bioprinter Using Direct-Volumetric Drop-On-Demand Technology for Fabricating Micro-Tissues and Drug-Delivery

Drop-on-demand (DOD) 3D bioprinting technologies currently hold the greatest promise for generating functional tissues for clinical use and for drug development. However, existing DOD 3D bioprinting technologies have three main limitations: (1) droplet volume inconsistency; (2) the ability to print...

Descripción completa

Detalles Bibliográficos
Autores principales: Grottkau, Brian E., Hui, Zhixin, Pang, Yonggang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7279004/
https://www.ncbi.nlm.nih.gov/pubmed/32423161
http://dx.doi.org/10.3390/ijms21103482
_version_ 1783543462543491072
author Grottkau, Brian E.
Hui, Zhixin
Pang, Yonggang
author_facet Grottkau, Brian E.
Hui, Zhixin
Pang, Yonggang
author_sort Grottkau, Brian E.
collection PubMed
description Drop-on-demand (DOD) 3D bioprinting technologies currently hold the greatest promise for generating functional tissues for clinical use and for drug development. However, existing DOD 3D bioprinting technologies have three main limitations: (1) droplet volume inconsistency; (2) the ability to print only bioinks with low cell concentrations and low viscosity; and (3) problems with cell viability when dispensed under high pressure. We report our success developing a novel direct-volumetric DOD (DVDOD) 3D bioprinting technology that overcomes each of these limitations. DVDOD can produce droplets of bioink from <10 nL in volume using a direct-volumetric mechanism with <± 5% volumetric percent accuracy in an accurate spatially controlled manner. DVDOD has the capability of dispensing bioinks with high concentrations of cells and/or high viscosity biomaterials in either low- or high-throughput modes. The cells are subjected to a low pressure during the bioprinting process for a very short period of time that does not negatively impact cell viability. We demonstrated the functions of the bioprinter in two distinct manners: (1) by using a high-throughput drug-delivery model; and (2) by bioprinting micro-tissues using a variety of different cell types, including functional micro-tissues of bone, cancer, and induced pluripotent stem cells. Our DVDOD technology demonstrates a promising platform for generating many types of tissues and drug-delivery models.
format Online
Article
Text
id pubmed-7279004
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-72790042020-06-15 A Novel 3D Bioprinter Using Direct-Volumetric Drop-On-Demand Technology for Fabricating Micro-Tissues and Drug-Delivery Grottkau, Brian E. Hui, Zhixin Pang, Yonggang Int J Mol Sci Article Drop-on-demand (DOD) 3D bioprinting technologies currently hold the greatest promise for generating functional tissues for clinical use and for drug development. However, existing DOD 3D bioprinting technologies have three main limitations: (1) droplet volume inconsistency; (2) the ability to print only bioinks with low cell concentrations and low viscosity; and (3) problems with cell viability when dispensed under high pressure. We report our success developing a novel direct-volumetric DOD (DVDOD) 3D bioprinting technology that overcomes each of these limitations. DVDOD can produce droplets of bioink from <10 nL in volume using a direct-volumetric mechanism with <± 5% volumetric percent accuracy in an accurate spatially controlled manner. DVDOD has the capability of dispensing bioinks with high concentrations of cells and/or high viscosity biomaterials in either low- or high-throughput modes. The cells are subjected to a low pressure during the bioprinting process for a very short period of time that does not negatively impact cell viability. We demonstrated the functions of the bioprinter in two distinct manners: (1) by using a high-throughput drug-delivery model; and (2) by bioprinting micro-tissues using a variety of different cell types, including functional micro-tissues of bone, cancer, and induced pluripotent stem cells. Our DVDOD technology demonstrates a promising platform for generating many types of tissues and drug-delivery models. MDPI 2020-05-14 /pmc/articles/PMC7279004/ /pubmed/32423161 http://dx.doi.org/10.3390/ijms21103482 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Grottkau, Brian E.
Hui, Zhixin
Pang, Yonggang
A Novel 3D Bioprinter Using Direct-Volumetric Drop-On-Demand Technology for Fabricating Micro-Tissues and Drug-Delivery
title A Novel 3D Bioprinter Using Direct-Volumetric Drop-On-Demand Technology for Fabricating Micro-Tissues and Drug-Delivery
title_full A Novel 3D Bioprinter Using Direct-Volumetric Drop-On-Demand Technology for Fabricating Micro-Tissues and Drug-Delivery
title_fullStr A Novel 3D Bioprinter Using Direct-Volumetric Drop-On-Demand Technology for Fabricating Micro-Tissues and Drug-Delivery
title_full_unstemmed A Novel 3D Bioprinter Using Direct-Volumetric Drop-On-Demand Technology for Fabricating Micro-Tissues and Drug-Delivery
title_short A Novel 3D Bioprinter Using Direct-Volumetric Drop-On-Demand Technology for Fabricating Micro-Tissues and Drug-Delivery
title_sort novel 3d bioprinter using direct-volumetric drop-on-demand technology for fabricating micro-tissues and drug-delivery
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7279004/
https://www.ncbi.nlm.nih.gov/pubmed/32423161
http://dx.doi.org/10.3390/ijms21103482
work_keys_str_mv AT grottkaubriane anovel3dbioprinterusingdirectvolumetricdropondemandtechnologyforfabricatingmicrotissuesanddrugdelivery
AT huizhixin anovel3dbioprinterusingdirectvolumetricdropondemandtechnologyforfabricatingmicrotissuesanddrugdelivery
AT pangyonggang anovel3dbioprinterusingdirectvolumetricdropondemandtechnologyforfabricatingmicrotissuesanddrugdelivery
AT grottkaubriane novel3dbioprinterusingdirectvolumetricdropondemandtechnologyforfabricatingmicrotissuesanddrugdelivery
AT huizhixin novel3dbioprinterusingdirectvolumetricdropondemandtechnologyforfabricatingmicrotissuesanddrugdelivery
AT pangyonggang novel3dbioprinterusingdirectvolumetricdropondemandtechnologyforfabricatingmicrotissuesanddrugdelivery