Cargando…

Increased Level of Vascular Endothelial Growth Factors by 4-hexylresorcinol is Mediated by Transforming Growth Factor-β1 and Accelerates Capillary Regeneration in the Burns in Diabetic Animals

4-Hexyl resorcinol (4HR) is an organic compound and has been used in skin care application. 4HR is an M2-type macrophage activator and elevates vascular endothelial growth factor (VEGF) expression via the hypoxia-inducible factor (HIF)-independent pathway. As endothelial cells are important in wound...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Dae-Won, Jo, You-Young, Garagiola, Umberto, Choi, Je-Yong, Kang, Yei-Jin, Oh, Ji-Hyeon, Kim, Seong-Gon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7279008/
https://www.ncbi.nlm.nih.gov/pubmed/32423083
http://dx.doi.org/10.3390/ijms21103473
_version_ 1783543463541735424
author Kim, Dae-Won
Jo, You-Young
Garagiola, Umberto
Choi, Je-Yong
Kang, Yei-Jin
Oh, Ji-Hyeon
Kim, Seong-Gon
author_facet Kim, Dae-Won
Jo, You-Young
Garagiola, Umberto
Choi, Je-Yong
Kang, Yei-Jin
Oh, Ji-Hyeon
Kim, Seong-Gon
author_sort Kim, Dae-Won
collection PubMed
description 4-Hexyl resorcinol (4HR) is an organic compound and has been used in skin care application. 4HR is an M2-type macrophage activator and elevates vascular endothelial growth factor (VEGF) expression via the hypoxia-inducible factor (HIF)-independent pathway. As endothelial cells are important in wound healing, the human umbilical vein endothelial cells (HUVECs) were treated with 4HR, and changes in VEGF-A, -C, and transforming growth factor-β1 (TGF-β1) expression were investigated. The administration of 4HR increased the expression level of VEGF-A, -C, and TGF-β1. The application of TGF-β1 protein also increased the expression level of VEGF-A and -C. Knockdown with small interfering RNA (siRNA) targeting to TGF-β1 and the selective chemical inhibition (A83-01) to ALK5 confirmed the involvement of the TGF-β signaling pathway in the 4-HR-mediated VEGFs expression. 4HR application in a burn model of diabetic rats demonstrated an increased level of angiogenic proteins with wound healing. Compared to sericin application, the 4HR application group showed more prominent capillary regeneration. Collectively, 4HR activated TGF-β1/ALK5/VEGFs signaling in endothelial cells and induced vascular regeneration and remodeling for wound healing.
format Online
Article
Text
id pubmed-7279008
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-72790082020-06-15 Increased Level of Vascular Endothelial Growth Factors by 4-hexylresorcinol is Mediated by Transforming Growth Factor-β1 and Accelerates Capillary Regeneration in the Burns in Diabetic Animals Kim, Dae-Won Jo, You-Young Garagiola, Umberto Choi, Je-Yong Kang, Yei-Jin Oh, Ji-Hyeon Kim, Seong-Gon Int J Mol Sci Article 4-Hexyl resorcinol (4HR) is an organic compound and has been used in skin care application. 4HR is an M2-type macrophage activator and elevates vascular endothelial growth factor (VEGF) expression via the hypoxia-inducible factor (HIF)-independent pathway. As endothelial cells are important in wound healing, the human umbilical vein endothelial cells (HUVECs) were treated with 4HR, and changes in VEGF-A, -C, and transforming growth factor-β1 (TGF-β1) expression were investigated. The administration of 4HR increased the expression level of VEGF-A, -C, and TGF-β1. The application of TGF-β1 protein also increased the expression level of VEGF-A and -C. Knockdown with small interfering RNA (siRNA) targeting to TGF-β1 and the selective chemical inhibition (A83-01) to ALK5 confirmed the involvement of the TGF-β signaling pathway in the 4-HR-mediated VEGFs expression. 4HR application in a burn model of diabetic rats demonstrated an increased level of angiogenic proteins with wound healing. Compared to sericin application, the 4HR application group showed more prominent capillary regeneration. Collectively, 4HR activated TGF-β1/ALK5/VEGFs signaling in endothelial cells and induced vascular regeneration and remodeling for wound healing. MDPI 2020-05-14 /pmc/articles/PMC7279008/ /pubmed/32423083 http://dx.doi.org/10.3390/ijms21103473 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Dae-Won
Jo, You-Young
Garagiola, Umberto
Choi, Je-Yong
Kang, Yei-Jin
Oh, Ji-Hyeon
Kim, Seong-Gon
Increased Level of Vascular Endothelial Growth Factors by 4-hexylresorcinol is Mediated by Transforming Growth Factor-β1 and Accelerates Capillary Regeneration in the Burns in Diabetic Animals
title Increased Level of Vascular Endothelial Growth Factors by 4-hexylresorcinol is Mediated by Transforming Growth Factor-β1 and Accelerates Capillary Regeneration in the Burns in Diabetic Animals
title_full Increased Level of Vascular Endothelial Growth Factors by 4-hexylresorcinol is Mediated by Transforming Growth Factor-β1 and Accelerates Capillary Regeneration in the Burns in Diabetic Animals
title_fullStr Increased Level of Vascular Endothelial Growth Factors by 4-hexylresorcinol is Mediated by Transforming Growth Factor-β1 and Accelerates Capillary Regeneration in the Burns in Diabetic Animals
title_full_unstemmed Increased Level of Vascular Endothelial Growth Factors by 4-hexylresorcinol is Mediated by Transforming Growth Factor-β1 and Accelerates Capillary Regeneration in the Burns in Diabetic Animals
title_short Increased Level of Vascular Endothelial Growth Factors by 4-hexylresorcinol is Mediated by Transforming Growth Factor-β1 and Accelerates Capillary Regeneration in the Burns in Diabetic Animals
title_sort increased level of vascular endothelial growth factors by 4-hexylresorcinol is mediated by transforming growth factor-β1 and accelerates capillary regeneration in the burns in diabetic animals
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7279008/
https://www.ncbi.nlm.nih.gov/pubmed/32423083
http://dx.doi.org/10.3390/ijms21103473
work_keys_str_mv AT kimdaewon increasedlevelofvascularendothelialgrowthfactorsby4hexylresorcinolismediatedbytransforminggrowthfactorb1andacceleratescapillaryregenerationintheburnsindiabeticanimals
AT joyouyoung increasedlevelofvascularendothelialgrowthfactorsby4hexylresorcinolismediatedbytransforminggrowthfactorb1andacceleratescapillaryregenerationintheburnsindiabeticanimals
AT garagiolaumberto increasedlevelofvascularendothelialgrowthfactorsby4hexylresorcinolismediatedbytransforminggrowthfactorb1andacceleratescapillaryregenerationintheburnsindiabeticanimals
AT choijeyong increasedlevelofvascularendothelialgrowthfactorsby4hexylresorcinolismediatedbytransforminggrowthfactorb1andacceleratescapillaryregenerationintheburnsindiabeticanimals
AT kangyeijin increasedlevelofvascularendothelialgrowthfactorsby4hexylresorcinolismediatedbytransforminggrowthfactorb1andacceleratescapillaryregenerationintheburnsindiabeticanimals
AT ohjihyeon increasedlevelofvascularendothelialgrowthfactorsby4hexylresorcinolismediatedbytransforminggrowthfactorb1andacceleratescapillaryregenerationintheburnsindiabeticanimals
AT kimseonggon increasedlevelofvascularendothelialgrowthfactorsby4hexylresorcinolismediatedbytransforminggrowthfactorb1andacceleratescapillaryregenerationintheburnsindiabeticanimals