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Ultrabright Fluorescent Silica Nanoparticles for Multiplexed Detection

Fluorescent tagging is a popular method in biomedical research. Using multiple taggants of different but resolvable fluorescent spectra simultaneously (multiplexing), it is possible to obtain more comprehensive and faster information about various biochemical reactions and diseases, for example, in...

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Autores principales: Peerzade, Saquib Ahmed M. A., Makarova, Nadezda, Sokolov, Igor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7279313/
https://www.ncbi.nlm.nih.gov/pubmed/32397124
http://dx.doi.org/10.3390/nano10050905
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author Peerzade, Saquib Ahmed M. A.
Makarova, Nadezda
Sokolov, Igor
author_facet Peerzade, Saquib Ahmed M. A.
Makarova, Nadezda
Sokolov, Igor
author_sort Peerzade, Saquib Ahmed M. A.
collection PubMed
description Fluorescent tagging is a popular method in biomedical research. Using multiple taggants of different but resolvable fluorescent spectra simultaneously (multiplexing), it is possible to obtain more comprehensive and faster information about various biochemical reactions and diseases, for example, in the method of flow cytometry. Here we report on a first demonstration of the synthesis of ultrabright fluorescent silica nanoporous nanoparticles (Star-dots), which have a large number of complex fluorescence spectra suitable for multiplexed applications. The spectra are obtained via simple physical mixing of different commercially available fluorescent dyes in a synthesizing bath. The resulting particles contain dye molecules encapsulated inside of cylindrical nanochannels of the silica matrix. The distance between the dye molecules is sufficiently small to attain Forster resonance energy transfer (FRET) coupling within a portion of the encapsulated dye molecules. As a result, one can have particles of multiple spectra that can be excited with just one wavelength. We show this for the mixing of five, three, and two dyes. Furthermore, the dyes can be mixed inside of particles in different proportions. This brings another dimension in the complexity of the obtained spectra and makes the number of different resolvable spectra practically unlimited. We demonstrate that the spectra obtained by different mixing of just two dyes inside of each particle can be easily distinguished by using a linear decomposition method. As a practical example, the errors of demultiplexing are measured when sets of a hundred particles are used for tagging.
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spelling pubmed-72793132020-06-17 Ultrabright Fluorescent Silica Nanoparticles for Multiplexed Detection Peerzade, Saquib Ahmed M. A. Makarova, Nadezda Sokolov, Igor Nanomaterials (Basel) Article Fluorescent tagging is a popular method in biomedical research. Using multiple taggants of different but resolvable fluorescent spectra simultaneously (multiplexing), it is possible to obtain more comprehensive and faster information about various biochemical reactions and diseases, for example, in the method of flow cytometry. Here we report on a first demonstration of the synthesis of ultrabright fluorescent silica nanoporous nanoparticles (Star-dots), which have a large number of complex fluorescence spectra suitable for multiplexed applications. The spectra are obtained via simple physical mixing of different commercially available fluorescent dyes in a synthesizing bath. The resulting particles contain dye molecules encapsulated inside of cylindrical nanochannels of the silica matrix. The distance between the dye molecules is sufficiently small to attain Forster resonance energy transfer (FRET) coupling within a portion of the encapsulated dye molecules. As a result, one can have particles of multiple spectra that can be excited with just one wavelength. We show this for the mixing of five, three, and two dyes. Furthermore, the dyes can be mixed inside of particles in different proportions. This brings another dimension in the complexity of the obtained spectra and makes the number of different resolvable spectra practically unlimited. We demonstrate that the spectra obtained by different mixing of just two dyes inside of each particle can be easily distinguished by using a linear decomposition method. As a practical example, the errors of demultiplexing are measured when sets of a hundred particles are used for tagging. MDPI 2020-05-08 /pmc/articles/PMC7279313/ /pubmed/32397124 http://dx.doi.org/10.3390/nano10050905 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Peerzade, Saquib Ahmed M. A.
Makarova, Nadezda
Sokolov, Igor
Ultrabright Fluorescent Silica Nanoparticles for Multiplexed Detection
title Ultrabright Fluorescent Silica Nanoparticles for Multiplexed Detection
title_full Ultrabright Fluorescent Silica Nanoparticles for Multiplexed Detection
title_fullStr Ultrabright Fluorescent Silica Nanoparticles for Multiplexed Detection
title_full_unstemmed Ultrabright Fluorescent Silica Nanoparticles for Multiplexed Detection
title_short Ultrabright Fluorescent Silica Nanoparticles for Multiplexed Detection
title_sort ultrabright fluorescent silica nanoparticles for multiplexed detection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7279313/
https://www.ncbi.nlm.nih.gov/pubmed/32397124
http://dx.doi.org/10.3390/nano10050905
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