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Ultrabright Fluorescent Silica Nanoparticles for Multiplexed Detection
Fluorescent tagging is a popular method in biomedical research. Using multiple taggants of different but resolvable fluorescent spectra simultaneously (multiplexing), it is possible to obtain more comprehensive and faster information about various biochemical reactions and diseases, for example, in...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7279313/ https://www.ncbi.nlm.nih.gov/pubmed/32397124 http://dx.doi.org/10.3390/nano10050905 |
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author | Peerzade, Saquib Ahmed M. A. Makarova, Nadezda Sokolov, Igor |
author_facet | Peerzade, Saquib Ahmed M. A. Makarova, Nadezda Sokolov, Igor |
author_sort | Peerzade, Saquib Ahmed M. A. |
collection | PubMed |
description | Fluorescent tagging is a popular method in biomedical research. Using multiple taggants of different but resolvable fluorescent spectra simultaneously (multiplexing), it is possible to obtain more comprehensive and faster information about various biochemical reactions and diseases, for example, in the method of flow cytometry. Here we report on a first demonstration of the synthesis of ultrabright fluorescent silica nanoporous nanoparticles (Star-dots), which have a large number of complex fluorescence spectra suitable for multiplexed applications. The spectra are obtained via simple physical mixing of different commercially available fluorescent dyes in a synthesizing bath. The resulting particles contain dye molecules encapsulated inside of cylindrical nanochannels of the silica matrix. The distance between the dye molecules is sufficiently small to attain Forster resonance energy transfer (FRET) coupling within a portion of the encapsulated dye molecules. As a result, one can have particles of multiple spectra that can be excited with just one wavelength. We show this for the mixing of five, three, and two dyes. Furthermore, the dyes can be mixed inside of particles in different proportions. This brings another dimension in the complexity of the obtained spectra and makes the number of different resolvable spectra practically unlimited. We demonstrate that the spectra obtained by different mixing of just two dyes inside of each particle can be easily distinguished by using a linear decomposition method. As a practical example, the errors of demultiplexing are measured when sets of a hundred particles are used for tagging. |
format | Online Article Text |
id | pubmed-7279313 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72793132020-06-17 Ultrabright Fluorescent Silica Nanoparticles for Multiplexed Detection Peerzade, Saquib Ahmed M. A. Makarova, Nadezda Sokolov, Igor Nanomaterials (Basel) Article Fluorescent tagging is a popular method in biomedical research. Using multiple taggants of different but resolvable fluorescent spectra simultaneously (multiplexing), it is possible to obtain more comprehensive and faster information about various biochemical reactions and diseases, for example, in the method of flow cytometry. Here we report on a first demonstration of the synthesis of ultrabright fluorescent silica nanoporous nanoparticles (Star-dots), which have a large number of complex fluorescence spectra suitable for multiplexed applications. The spectra are obtained via simple physical mixing of different commercially available fluorescent dyes in a synthesizing bath. The resulting particles contain dye molecules encapsulated inside of cylindrical nanochannels of the silica matrix. The distance between the dye molecules is sufficiently small to attain Forster resonance energy transfer (FRET) coupling within a portion of the encapsulated dye molecules. As a result, one can have particles of multiple spectra that can be excited with just one wavelength. We show this for the mixing of five, three, and two dyes. Furthermore, the dyes can be mixed inside of particles in different proportions. This brings another dimension in the complexity of the obtained spectra and makes the number of different resolvable spectra practically unlimited. We demonstrate that the spectra obtained by different mixing of just two dyes inside of each particle can be easily distinguished by using a linear decomposition method. As a practical example, the errors of demultiplexing are measured when sets of a hundred particles are used for tagging. MDPI 2020-05-08 /pmc/articles/PMC7279313/ /pubmed/32397124 http://dx.doi.org/10.3390/nano10050905 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Peerzade, Saquib Ahmed M. A. Makarova, Nadezda Sokolov, Igor Ultrabright Fluorescent Silica Nanoparticles for Multiplexed Detection |
title | Ultrabright Fluorescent Silica Nanoparticles for Multiplexed Detection |
title_full | Ultrabright Fluorescent Silica Nanoparticles for Multiplexed Detection |
title_fullStr | Ultrabright Fluorescent Silica Nanoparticles for Multiplexed Detection |
title_full_unstemmed | Ultrabright Fluorescent Silica Nanoparticles for Multiplexed Detection |
title_short | Ultrabright Fluorescent Silica Nanoparticles for Multiplexed Detection |
title_sort | ultrabright fluorescent silica nanoparticles for multiplexed detection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7279313/ https://www.ncbi.nlm.nih.gov/pubmed/32397124 http://dx.doi.org/10.3390/nano10050905 |
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