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Towards Microorganism-Based Biofuel Cells: The Viability of Saccharomyces cerevisiae Modified by Multiwalled Carbon Nanotubes †

This research aimed to evaluate the toxic effect of multi-walled carbon nanotubes (MW-CNTs) on yeast cells in order to apply MW-CNTs for possible improvement of the efficiency of microbial biofuel cells. The SEM and XRD analysis suggested that here used MW-CNTs are in the range of 10–25 nm in diamet...

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Autores principales: Bruzaite, Ingrida, Rozene, Juste, Morkvenaite-Vilkonciene, Inga, Ramanavicius, Arunas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7279342/
https://www.ncbi.nlm.nih.gov/pubmed/32429594
http://dx.doi.org/10.3390/nano10050954
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author Bruzaite, Ingrida
Rozene, Juste
Morkvenaite-Vilkonciene, Inga
Ramanavicius, Arunas
author_facet Bruzaite, Ingrida
Rozene, Juste
Morkvenaite-Vilkonciene, Inga
Ramanavicius, Arunas
author_sort Bruzaite, Ingrida
collection PubMed
description This research aimed to evaluate the toxic effect of multi-walled carbon nanotubes (MW-CNTs) on yeast cells in order to apply MW-CNTs for possible improvement of the efficiency of microbial biofuel cells. The SEM and XRD analysis suggested that here used MW-CNTs are in the range of 10–25 nm in diameter and their structure was confirmed by Raman spectroscopy. In this study, we evaluated the viability of the yeast Saccharomyces cerevisiae cells, affected by MW-CNTs, by cell count, culture optical density and atomic force microscopy. The yeast cells were exposed towards MW-CNTs (of 2, 50, 100 μg/mL concentrations in water-based solution) for 24 h. A mathematical model was applied for the evaluation of relative growth and relative death rates of yeast cells. We calculated that both of the rates are two times higher in the case if yeasts were treated by 50, 100 μg/mL of MW-CNTs containing solution, comparing to that treated by 0 and 2 μg/mL c of MW-CNTs containing solution. It was determined that the MW-CNTs have some observable effect upon the incubation of the yeast cells. The viability of yeast has decreased together with MW-CNTs concentration only after 5 h of the treatment. Therefore, we predict that the MW-CNTs can be applied for the modification of yeast cells in order to improve electrical charge transfer through the yeast cell membrane and/or the cell wall.
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spelling pubmed-72793422020-06-17 Towards Microorganism-Based Biofuel Cells: The Viability of Saccharomyces cerevisiae Modified by Multiwalled Carbon Nanotubes † Bruzaite, Ingrida Rozene, Juste Morkvenaite-Vilkonciene, Inga Ramanavicius, Arunas Nanomaterials (Basel) Article This research aimed to evaluate the toxic effect of multi-walled carbon nanotubes (MW-CNTs) on yeast cells in order to apply MW-CNTs for possible improvement of the efficiency of microbial biofuel cells. The SEM and XRD analysis suggested that here used MW-CNTs are in the range of 10–25 nm in diameter and their structure was confirmed by Raman spectroscopy. In this study, we evaluated the viability of the yeast Saccharomyces cerevisiae cells, affected by MW-CNTs, by cell count, culture optical density and atomic force microscopy. The yeast cells were exposed towards MW-CNTs (of 2, 50, 100 μg/mL concentrations in water-based solution) for 24 h. A mathematical model was applied for the evaluation of relative growth and relative death rates of yeast cells. We calculated that both of the rates are two times higher in the case if yeasts were treated by 50, 100 μg/mL of MW-CNTs containing solution, comparing to that treated by 0 and 2 μg/mL c of MW-CNTs containing solution. It was determined that the MW-CNTs have some observable effect upon the incubation of the yeast cells. The viability of yeast has decreased together with MW-CNTs concentration only after 5 h of the treatment. Therefore, we predict that the MW-CNTs can be applied for the modification of yeast cells in order to improve electrical charge transfer through the yeast cell membrane and/or the cell wall. MDPI 2020-05-17 /pmc/articles/PMC7279342/ /pubmed/32429594 http://dx.doi.org/10.3390/nano10050954 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bruzaite, Ingrida
Rozene, Juste
Morkvenaite-Vilkonciene, Inga
Ramanavicius, Arunas
Towards Microorganism-Based Biofuel Cells: The Viability of Saccharomyces cerevisiae Modified by Multiwalled Carbon Nanotubes †
title Towards Microorganism-Based Biofuel Cells: The Viability of Saccharomyces cerevisiae Modified by Multiwalled Carbon Nanotubes †
title_full Towards Microorganism-Based Biofuel Cells: The Viability of Saccharomyces cerevisiae Modified by Multiwalled Carbon Nanotubes †
title_fullStr Towards Microorganism-Based Biofuel Cells: The Viability of Saccharomyces cerevisiae Modified by Multiwalled Carbon Nanotubes †
title_full_unstemmed Towards Microorganism-Based Biofuel Cells: The Viability of Saccharomyces cerevisiae Modified by Multiwalled Carbon Nanotubes †
title_short Towards Microorganism-Based Biofuel Cells: The Viability of Saccharomyces cerevisiae Modified by Multiwalled Carbon Nanotubes †
title_sort towards microorganism-based biofuel cells: the viability of saccharomyces cerevisiae modified by multiwalled carbon nanotubes †
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7279342/
https://www.ncbi.nlm.nih.gov/pubmed/32429594
http://dx.doi.org/10.3390/nano10050954
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