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Antioxidant Strategy to Prevent Simulated Microgravity-Induced Effects on Bone Osteoblasts
The effects induced by microgravity on human body functions have been widely described, in particular those on skeletal muscle and bone tissues. This study aims to implement information on the possible countermeasures necessary to neutralize the oxidative imbalance induced by microgravity on osteobl...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7279347/ https://www.ncbi.nlm.nih.gov/pubmed/32455731 http://dx.doi.org/10.3390/ijms21103638 |
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author | Morabito, Caterina Guarnieri, Simone Cucina, Alessandra Bizzarri, Mariano Mariggiò, Maria A. |
author_facet | Morabito, Caterina Guarnieri, Simone Cucina, Alessandra Bizzarri, Mariano Mariggiò, Maria A. |
author_sort | Morabito, Caterina |
collection | PubMed |
description | The effects induced by microgravity on human body functions have been widely described, in particular those on skeletal muscle and bone tissues. This study aims to implement information on the possible countermeasures necessary to neutralize the oxidative imbalance induced by microgravity on osteoblastic cells. Using the model of murine MC3T3-E1 osteoblast cells, cellular morphology, proliferation, and metabolism were investigated during exposure to simulated microgravity on a random positioning machine in the absence or presence of an antioxidant—the 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox). Our results confirm that simulated microgravity-induced morphological and metabolic alterations characterized by increased levels of reactive oxygen species and a slowdown of the proliferative rate. Interestingly, the use of Trolox inhibited the simulated microgravity-induced effects. Indeed, the antioxidant-neutralizing oxidants preserved cell cytoskeletal architecture and restored cell proliferation rate and metabolism. The use of appropriate antioxidant countermeasures could prevent the modifications and damage induced by microgravity on osteoblastic cells and consequently on bone homeostasis. |
format | Online Article Text |
id | pubmed-7279347 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72793472020-06-17 Antioxidant Strategy to Prevent Simulated Microgravity-Induced Effects on Bone Osteoblasts Morabito, Caterina Guarnieri, Simone Cucina, Alessandra Bizzarri, Mariano Mariggiò, Maria A. Int J Mol Sci Article The effects induced by microgravity on human body functions have been widely described, in particular those on skeletal muscle and bone tissues. This study aims to implement information on the possible countermeasures necessary to neutralize the oxidative imbalance induced by microgravity on osteoblastic cells. Using the model of murine MC3T3-E1 osteoblast cells, cellular morphology, proliferation, and metabolism were investigated during exposure to simulated microgravity on a random positioning machine in the absence or presence of an antioxidant—the 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox). Our results confirm that simulated microgravity-induced morphological and metabolic alterations characterized by increased levels of reactive oxygen species and a slowdown of the proliferative rate. Interestingly, the use of Trolox inhibited the simulated microgravity-induced effects. Indeed, the antioxidant-neutralizing oxidants preserved cell cytoskeletal architecture and restored cell proliferation rate and metabolism. The use of appropriate antioxidant countermeasures could prevent the modifications and damage induced by microgravity on osteoblastic cells and consequently on bone homeostasis. MDPI 2020-05-21 /pmc/articles/PMC7279347/ /pubmed/32455731 http://dx.doi.org/10.3390/ijms21103638 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Morabito, Caterina Guarnieri, Simone Cucina, Alessandra Bizzarri, Mariano Mariggiò, Maria A. Antioxidant Strategy to Prevent Simulated Microgravity-Induced Effects on Bone Osteoblasts |
title | Antioxidant Strategy to Prevent Simulated Microgravity-Induced Effects on Bone Osteoblasts |
title_full | Antioxidant Strategy to Prevent Simulated Microgravity-Induced Effects on Bone Osteoblasts |
title_fullStr | Antioxidant Strategy to Prevent Simulated Microgravity-Induced Effects on Bone Osteoblasts |
title_full_unstemmed | Antioxidant Strategy to Prevent Simulated Microgravity-Induced Effects on Bone Osteoblasts |
title_short | Antioxidant Strategy to Prevent Simulated Microgravity-Induced Effects on Bone Osteoblasts |
title_sort | antioxidant strategy to prevent simulated microgravity-induced effects on bone osteoblasts |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7279347/ https://www.ncbi.nlm.nih.gov/pubmed/32455731 http://dx.doi.org/10.3390/ijms21103638 |
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