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Lon Protease Is Important for Growth under Stressful Conditions and Pathogenicity of the Phytopathogen, Bacterium Dickeya solani

The Lon protein is a protease implicated in the virulence of many pathogenic bacteria, including some plant pathogens. However, little is known about the role of Lon in bacteria from genus Dickeya. This group of bacteria includes important potato pathogens, with the most aggressive species, D. solan...

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Autores principales: Figaj, Donata, Czaplewska, Paulina, Przepióra, Tomasz, Ambroziak, Patrycja, Potrykus, Marta, Skorko-Glonek, Joanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7279449/
https://www.ncbi.nlm.nih.gov/pubmed/32456249
http://dx.doi.org/10.3390/ijms21103687
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author Figaj, Donata
Czaplewska, Paulina
Przepióra, Tomasz
Ambroziak, Patrycja
Potrykus, Marta
Skorko-Glonek, Joanna
author_facet Figaj, Donata
Czaplewska, Paulina
Przepióra, Tomasz
Ambroziak, Patrycja
Potrykus, Marta
Skorko-Glonek, Joanna
author_sort Figaj, Donata
collection PubMed
description The Lon protein is a protease implicated in the virulence of many pathogenic bacteria, including some plant pathogens. However, little is known about the role of Lon in bacteria from genus Dickeya. This group of bacteria includes important potato pathogens, with the most aggressive species, D. solani. To determine the importance of Lon for pathogenicity and response to stress conditions of bacteria, we constructed a D. solani Δlon strain. The mutant bacteria showed increased sensitivity to certain stress conditions, in particular osmotic and high-temperature stresses. Furthermore, qPCR analysis showed an increased expression of the lon gene in D. solani under these conditions. The deletion of the lon gene resulted in decreased motility, lower activity of secreted pectinolytic enzymes and finally delayed onset of blackleg symptoms in the potato plants. In the Δlon cells, the altered levels of several proteins, including virulence factors and proteins associated with virulence, were detected by means of Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH-MS) analysis. These included components of the type III secretion system and proteins involved in bacterial motility. Our results indicate that Lon protease is important for D. solani to withstand stressful conditions and effectively invade the potato plant.
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spelling pubmed-72794492020-06-17 Lon Protease Is Important for Growth under Stressful Conditions and Pathogenicity of the Phytopathogen, Bacterium Dickeya solani Figaj, Donata Czaplewska, Paulina Przepióra, Tomasz Ambroziak, Patrycja Potrykus, Marta Skorko-Glonek, Joanna Int J Mol Sci Article The Lon protein is a protease implicated in the virulence of many pathogenic bacteria, including some plant pathogens. However, little is known about the role of Lon in bacteria from genus Dickeya. This group of bacteria includes important potato pathogens, with the most aggressive species, D. solani. To determine the importance of Lon for pathogenicity and response to stress conditions of bacteria, we constructed a D. solani Δlon strain. The mutant bacteria showed increased sensitivity to certain stress conditions, in particular osmotic and high-temperature stresses. Furthermore, qPCR analysis showed an increased expression of the lon gene in D. solani under these conditions. The deletion of the lon gene resulted in decreased motility, lower activity of secreted pectinolytic enzymes and finally delayed onset of blackleg symptoms in the potato plants. In the Δlon cells, the altered levels of several proteins, including virulence factors and proteins associated with virulence, were detected by means of Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH-MS) analysis. These included components of the type III secretion system and proteins involved in bacterial motility. Our results indicate that Lon protease is important for D. solani to withstand stressful conditions and effectively invade the potato plant. MDPI 2020-05-23 /pmc/articles/PMC7279449/ /pubmed/32456249 http://dx.doi.org/10.3390/ijms21103687 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Figaj, Donata
Czaplewska, Paulina
Przepióra, Tomasz
Ambroziak, Patrycja
Potrykus, Marta
Skorko-Glonek, Joanna
Lon Protease Is Important for Growth under Stressful Conditions and Pathogenicity of the Phytopathogen, Bacterium Dickeya solani
title Lon Protease Is Important for Growth under Stressful Conditions and Pathogenicity of the Phytopathogen, Bacterium Dickeya solani
title_full Lon Protease Is Important for Growth under Stressful Conditions and Pathogenicity of the Phytopathogen, Bacterium Dickeya solani
title_fullStr Lon Protease Is Important for Growth under Stressful Conditions and Pathogenicity of the Phytopathogen, Bacterium Dickeya solani
title_full_unstemmed Lon Protease Is Important for Growth under Stressful Conditions and Pathogenicity of the Phytopathogen, Bacterium Dickeya solani
title_short Lon Protease Is Important for Growth under Stressful Conditions and Pathogenicity of the Phytopathogen, Bacterium Dickeya solani
title_sort lon protease is important for growth under stressful conditions and pathogenicity of the phytopathogen, bacterium dickeya solani
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7279449/
https://www.ncbi.nlm.nih.gov/pubmed/32456249
http://dx.doi.org/10.3390/ijms21103687
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