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ABO blood group is a determinant of von Willebrand factor protein levels in human pulmonary endothelial cells

ABO blood group antigens are expressed on von Willebrand factor (VWF) and glycosylation patterns influence circulating VWF levels. The aim of this study was to examine the effect of ABO blood type on tissue-associated VWF protein levels. We selected 35 formalin-fixed paraffin-embedded pulmonary tiss...

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Autores principales: Murray, Glenn P, Post, Steven R, Post, Ginell R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BMJ Publishing Group 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7279561/
https://www.ncbi.nlm.nih.gov/pubmed/31662441
http://dx.doi.org/10.1136/jclinpath-2019-206182
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author Murray, Glenn P
Post, Steven R
Post, Ginell R
author_facet Murray, Glenn P
Post, Steven R
Post, Ginell R
author_sort Murray, Glenn P
collection PubMed
description ABO blood group antigens are expressed on von Willebrand factor (VWF) and glycosylation patterns influence circulating VWF levels. The aim of this study was to examine the effect of ABO blood type on tissue-associated VWF protein levels. We selected 35 formalin-fixed paraffin-embedded pulmonary tissue blocks obtained at autopsy from decedents who died from pulmonary embolism with known ABO blood groups (O, A, B and AB phenotypes), prepared tissue microarrays (TMAs) and stained TMAs with antibodies to VWF and platelet/endothelial cell adhesion marker-1 (PECAM-1) as a marker of endothelial cells. A pixel count scoring algorithm was used to quantify VWF and PECAM-1 staining intensity in pulmonary arterioles in digitised images. Compared with type O, non-O individuals have a significantly higher amount of endothelial cell-associated VWF protein expression. VWF protein levels associated with pulmonary vascular endothelial cells is influenced by ABO antigenic determinants.
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spelling pubmed-72795612020-06-15 ABO blood group is a determinant of von Willebrand factor protein levels in human pulmonary endothelial cells Murray, Glenn P Post, Steven R Post, Ginell R J Clin Pathol Short Report ABO blood group antigens are expressed on von Willebrand factor (VWF) and glycosylation patterns influence circulating VWF levels. The aim of this study was to examine the effect of ABO blood type on tissue-associated VWF protein levels. We selected 35 formalin-fixed paraffin-embedded pulmonary tissue blocks obtained at autopsy from decedents who died from pulmonary embolism with known ABO blood groups (O, A, B and AB phenotypes), prepared tissue microarrays (TMAs) and stained TMAs with antibodies to VWF and platelet/endothelial cell adhesion marker-1 (PECAM-1) as a marker of endothelial cells. A pixel count scoring algorithm was used to quantify VWF and PECAM-1 staining intensity in pulmonary arterioles in digitised images. Compared with type O, non-O individuals have a significantly higher amount of endothelial cell-associated VWF protein expression. VWF protein levels associated with pulmonary vascular endothelial cells is influenced by ABO antigenic determinants. BMJ Publishing Group 2020-06 2019-10-29 /pmc/articles/PMC7279561/ /pubmed/31662441 http://dx.doi.org/10.1136/jclinpath-2019-206182 Text en © Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ. http://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Short Report
Murray, Glenn P
Post, Steven R
Post, Ginell R
ABO blood group is a determinant of von Willebrand factor protein levels in human pulmonary endothelial cells
title ABO blood group is a determinant of von Willebrand factor protein levels in human pulmonary endothelial cells
title_full ABO blood group is a determinant of von Willebrand factor protein levels in human pulmonary endothelial cells
title_fullStr ABO blood group is a determinant of von Willebrand factor protein levels in human pulmonary endothelial cells
title_full_unstemmed ABO blood group is a determinant of von Willebrand factor protein levels in human pulmonary endothelial cells
title_short ABO blood group is a determinant of von Willebrand factor protein levels in human pulmonary endothelial cells
title_sort abo blood group is a determinant of von willebrand factor protein levels in human pulmonary endothelial cells
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7279561/
https://www.ncbi.nlm.nih.gov/pubmed/31662441
http://dx.doi.org/10.1136/jclinpath-2019-206182
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