Cargando…
Azithromycin decreases Chlamydia pneumoniae-mediated Interleukin-4 responses but not Immunoglobulin E responses
BACKGROUND: Chlamydia pneumoniae is an obligate intracellular bacterium that causes respiratory infection. There may exist an association between C. pneumoniae, asthma, and production of immunoglobulin (Ig) E responses in vitro. Interleukin (IL-4) is required for IgE production. OBJECTIVE: We previo...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7279567/ https://www.ncbi.nlm.nih.gov/pubmed/32511255 http://dx.doi.org/10.1371/journal.pone.0234413 |
_version_ | 1783543590417334272 |
---|---|
author | Smith-Norowitz, Tamar A. Huang, Yvonne Loeffler, Jeffrey Klein, Elliot Norowitz, Yitzchok M. Hammerschlag, Margaret R. Joks, Rauno Kohlhoff, Stephan |
author_facet | Smith-Norowitz, Tamar A. Huang, Yvonne Loeffler, Jeffrey Klein, Elliot Norowitz, Yitzchok M. Hammerschlag, Margaret R. Joks, Rauno Kohlhoff, Stephan |
author_sort | Smith-Norowitz, Tamar A. |
collection | PubMed |
description | BACKGROUND: Chlamydia pneumoniae is an obligate intracellular bacterium that causes respiratory infection. There may exist an association between C. pneumoniae, asthma, and production of immunoglobulin (Ig) E responses in vitro. Interleukin (IL-4) is required for IgE production. OBJECTIVE: We previously demonstrated that doxycycline suppresses C. pneumoniae-induced production of IgE and IL-4 responses in peripheral blood mononuclear cells (PBMC) from asthmatic subjects. Whereas macrolides have anti-chlamydial activity, their effect on in vitro anti-inflammatory (IgE) and IL-4 responses to C. pneumoniae have not been studied. METHODS: PBMC from IgE- adult atopic subjects (N = 5) were infected +/- C. pneumoniae BAL69, +/- azithromycin (0.1, 1.0 ug/mL) for 10 days. IL-4 and IgE levels were determined in supernatants by ELISA. IL-4 and IgE were detected in supernatants of PBMC (day 10). RESULTS: When azithromycin (0.1, 1.0 ug/ml) was added, IL-4 levels decreased. At low dose, IgE levels increased and at high dose, IgE levels decreased. When PBMC were infected with C. pneumoniae, both IL-4 and IgE levels decreased. Addition of azithromycin (0.1, 1.0 ug/mL) decreased IL-4 levels and had no effect on IgE levels. CONCLUSIONS: These findings indicate that azithromycin decreases IL-4 responses but has a bimodal effect on IgE responses in PBMC from atopic patients in vitro. |
format | Online Article Text |
id | pubmed-7279567 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-72795672020-06-17 Azithromycin decreases Chlamydia pneumoniae-mediated Interleukin-4 responses but not Immunoglobulin E responses Smith-Norowitz, Tamar A. Huang, Yvonne Loeffler, Jeffrey Klein, Elliot Norowitz, Yitzchok M. Hammerschlag, Margaret R. Joks, Rauno Kohlhoff, Stephan PLoS One Research Article BACKGROUND: Chlamydia pneumoniae is an obligate intracellular bacterium that causes respiratory infection. There may exist an association between C. pneumoniae, asthma, and production of immunoglobulin (Ig) E responses in vitro. Interleukin (IL-4) is required for IgE production. OBJECTIVE: We previously demonstrated that doxycycline suppresses C. pneumoniae-induced production of IgE and IL-4 responses in peripheral blood mononuclear cells (PBMC) from asthmatic subjects. Whereas macrolides have anti-chlamydial activity, their effect on in vitro anti-inflammatory (IgE) and IL-4 responses to C. pneumoniae have not been studied. METHODS: PBMC from IgE- adult atopic subjects (N = 5) were infected +/- C. pneumoniae BAL69, +/- azithromycin (0.1, 1.0 ug/mL) for 10 days. IL-4 and IgE levels were determined in supernatants by ELISA. IL-4 and IgE were detected in supernatants of PBMC (day 10). RESULTS: When azithromycin (0.1, 1.0 ug/ml) was added, IL-4 levels decreased. At low dose, IgE levels increased and at high dose, IgE levels decreased. When PBMC were infected with C. pneumoniae, both IL-4 and IgE levels decreased. Addition of azithromycin (0.1, 1.0 ug/mL) decreased IL-4 levels and had no effect on IgE levels. CONCLUSIONS: These findings indicate that azithromycin decreases IL-4 responses but has a bimodal effect on IgE responses in PBMC from atopic patients in vitro. Public Library of Science 2020-06-08 /pmc/articles/PMC7279567/ /pubmed/32511255 http://dx.doi.org/10.1371/journal.pone.0234413 Text en © 2020 Smith-Norowitz et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Smith-Norowitz, Tamar A. Huang, Yvonne Loeffler, Jeffrey Klein, Elliot Norowitz, Yitzchok M. Hammerschlag, Margaret R. Joks, Rauno Kohlhoff, Stephan Azithromycin decreases Chlamydia pneumoniae-mediated Interleukin-4 responses but not Immunoglobulin E responses |
title | Azithromycin decreases Chlamydia pneumoniae-mediated Interleukin-4 responses but not Immunoglobulin E responses |
title_full | Azithromycin decreases Chlamydia pneumoniae-mediated Interleukin-4 responses but not Immunoglobulin E responses |
title_fullStr | Azithromycin decreases Chlamydia pneumoniae-mediated Interleukin-4 responses but not Immunoglobulin E responses |
title_full_unstemmed | Azithromycin decreases Chlamydia pneumoniae-mediated Interleukin-4 responses but not Immunoglobulin E responses |
title_short | Azithromycin decreases Chlamydia pneumoniae-mediated Interleukin-4 responses but not Immunoglobulin E responses |
title_sort | azithromycin decreases chlamydia pneumoniae-mediated interleukin-4 responses but not immunoglobulin e responses |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7279567/ https://www.ncbi.nlm.nih.gov/pubmed/32511255 http://dx.doi.org/10.1371/journal.pone.0234413 |
work_keys_str_mv | AT smithnorowitztamara azithromycindecreaseschlamydiapneumoniaemediatedinterleukin4responsesbutnotimmunoglobulineresponses AT huangyvonne azithromycindecreaseschlamydiapneumoniaemediatedinterleukin4responsesbutnotimmunoglobulineresponses AT loefflerjeffrey azithromycindecreaseschlamydiapneumoniaemediatedinterleukin4responsesbutnotimmunoglobulineresponses AT kleinelliot azithromycindecreaseschlamydiapneumoniaemediatedinterleukin4responsesbutnotimmunoglobulineresponses AT norowitzyitzchokm azithromycindecreaseschlamydiapneumoniaemediatedinterleukin4responsesbutnotimmunoglobulineresponses AT hammerschlagmargaretr azithromycindecreaseschlamydiapneumoniaemediatedinterleukin4responsesbutnotimmunoglobulineresponses AT joksrauno azithromycindecreaseschlamydiapneumoniaemediatedinterleukin4responsesbutnotimmunoglobulineresponses AT kohlhoffstephan azithromycindecreaseschlamydiapneumoniaemediatedinterleukin4responsesbutnotimmunoglobulineresponses |