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Azithromycin decreases Chlamydia pneumoniae-mediated Interleukin-4 responses but not Immunoglobulin E responses

BACKGROUND: Chlamydia pneumoniae is an obligate intracellular bacterium that causes respiratory infection. There may exist an association between C. pneumoniae, asthma, and production of immunoglobulin (Ig) E responses in vitro. Interleukin (IL-4) is required for IgE production. OBJECTIVE: We previo...

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Autores principales: Smith-Norowitz, Tamar A., Huang, Yvonne, Loeffler, Jeffrey, Klein, Elliot, Norowitz, Yitzchok M., Hammerschlag, Margaret R., Joks, Rauno, Kohlhoff, Stephan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7279567/
https://www.ncbi.nlm.nih.gov/pubmed/32511255
http://dx.doi.org/10.1371/journal.pone.0234413
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author Smith-Norowitz, Tamar A.
Huang, Yvonne
Loeffler, Jeffrey
Klein, Elliot
Norowitz, Yitzchok M.
Hammerschlag, Margaret R.
Joks, Rauno
Kohlhoff, Stephan
author_facet Smith-Norowitz, Tamar A.
Huang, Yvonne
Loeffler, Jeffrey
Klein, Elliot
Norowitz, Yitzchok M.
Hammerschlag, Margaret R.
Joks, Rauno
Kohlhoff, Stephan
author_sort Smith-Norowitz, Tamar A.
collection PubMed
description BACKGROUND: Chlamydia pneumoniae is an obligate intracellular bacterium that causes respiratory infection. There may exist an association between C. pneumoniae, asthma, and production of immunoglobulin (Ig) E responses in vitro. Interleukin (IL-4) is required for IgE production. OBJECTIVE: We previously demonstrated that doxycycline suppresses C. pneumoniae-induced production of IgE and IL-4 responses in peripheral blood mononuclear cells (PBMC) from asthmatic subjects. Whereas macrolides have anti-chlamydial activity, their effect on in vitro anti-inflammatory (IgE) and IL-4 responses to C. pneumoniae have not been studied. METHODS: PBMC from IgE- adult atopic subjects (N = 5) were infected +/- C. pneumoniae BAL69, +/- azithromycin (0.1, 1.0 ug/mL) for 10 days. IL-4 and IgE levels were determined in supernatants by ELISA. IL-4 and IgE were detected in supernatants of PBMC (day 10). RESULTS: When azithromycin (0.1, 1.0 ug/ml) was added, IL-4 levels decreased. At low dose, IgE levels increased and at high dose, IgE levels decreased. When PBMC were infected with C. pneumoniae, both IL-4 and IgE levels decreased. Addition of azithromycin (0.1, 1.0 ug/mL) decreased IL-4 levels and had no effect on IgE levels. CONCLUSIONS: These findings indicate that azithromycin decreases IL-4 responses but has a bimodal effect on IgE responses in PBMC from atopic patients in vitro.
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spelling pubmed-72795672020-06-17 Azithromycin decreases Chlamydia pneumoniae-mediated Interleukin-4 responses but not Immunoglobulin E responses Smith-Norowitz, Tamar A. Huang, Yvonne Loeffler, Jeffrey Klein, Elliot Norowitz, Yitzchok M. Hammerschlag, Margaret R. Joks, Rauno Kohlhoff, Stephan PLoS One Research Article BACKGROUND: Chlamydia pneumoniae is an obligate intracellular bacterium that causes respiratory infection. There may exist an association between C. pneumoniae, asthma, and production of immunoglobulin (Ig) E responses in vitro. Interleukin (IL-4) is required for IgE production. OBJECTIVE: We previously demonstrated that doxycycline suppresses C. pneumoniae-induced production of IgE and IL-4 responses in peripheral blood mononuclear cells (PBMC) from asthmatic subjects. Whereas macrolides have anti-chlamydial activity, their effect on in vitro anti-inflammatory (IgE) and IL-4 responses to C. pneumoniae have not been studied. METHODS: PBMC from IgE- adult atopic subjects (N = 5) were infected +/- C. pneumoniae BAL69, +/- azithromycin (0.1, 1.0 ug/mL) for 10 days. IL-4 and IgE levels were determined in supernatants by ELISA. IL-4 and IgE were detected in supernatants of PBMC (day 10). RESULTS: When azithromycin (0.1, 1.0 ug/ml) was added, IL-4 levels decreased. At low dose, IgE levels increased and at high dose, IgE levels decreased. When PBMC were infected with C. pneumoniae, both IL-4 and IgE levels decreased. Addition of azithromycin (0.1, 1.0 ug/mL) decreased IL-4 levels and had no effect on IgE levels. CONCLUSIONS: These findings indicate that azithromycin decreases IL-4 responses but has a bimodal effect on IgE responses in PBMC from atopic patients in vitro. Public Library of Science 2020-06-08 /pmc/articles/PMC7279567/ /pubmed/32511255 http://dx.doi.org/10.1371/journal.pone.0234413 Text en © 2020 Smith-Norowitz et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Smith-Norowitz, Tamar A.
Huang, Yvonne
Loeffler, Jeffrey
Klein, Elliot
Norowitz, Yitzchok M.
Hammerschlag, Margaret R.
Joks, Rauno
Kohlhoff, Stephan
Azithromycin decreases Chlamydia pneumoniae-mediated Interleukin-4 responses but not Immunoglobulin E responses
title Azithromycin decreases Chlamydia pneumoniae-mediated Interleukin-4 responses but not Immunoglobulin E responses
title_full Azithromycin decreases Chlamydia pneumoniae-mediated Interleukin-4 responses but not Immunoglobulin E responses
title_fullStr Azithromycin decreases Chlamydia pneumoniae-mediated Interleukin-4 responses but not Immunoglobulin E responses
title_full_unstemmed Azithromycin decreases Chlamydia pneumoniae-mediated Interleukin-4 responses but not Immunoglobulin E responses
title_short Azithromycin decreases Chlamydia pneumoniae-mediated Interleukin-4 responses but not Immunoglobulin E responses
title_sort azithromycin decreases chlamydia pneumoniae-mediated interleukin-4 responses but not immunoglobulin e responses
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7279567/
https://www.ncbi.nlm.nih.gov/pubmed/32511255
http://dx.doi.org/10.1371/journal.pone.0234413
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