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The Ralstonia solanacearum effector RipI induces a defence reaction by interacting with the bHLH93 transcription factor in Nicotiana benthamiana
Ralstonia solanacearum releases a set of effectors into plant cells that modify the host defence reaction. The role of the effector protein RipI during infection has not been elucidated. In this study, we demonstrated that transient overexpression of RipI induces the hypersensitive response (HR), up...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7279998/ https://www.ncbi.nlm.nih.gov/pubmed/32285606 http://dx.doi.org/10.1111/mpp.12937 |
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author | Zhuo, Tao Wang, Xue Chen, Zhengyu Cui, Haitao Zeng, Yanhong Chen, Yang Fan, Xiaojing Hu, Xun Zou, Huasong |
author_facet | Zhuo, Tao Wang, Xue Chen, Zhengyu Cui, Haitao Zeng, Yanhong Chen, Yang Fan, Xiaojing Hu, Xun Zou, Huasong |
author_sort | Zhuo, Tao |
collection | PubMed |
description | Ralstonia solanacearum releases a set of effectors into plant cells that modify the host defence reaction. The role of the effector protein RipI during infection has not been elucidated. In this study, we demonstrated that transient overexpression of RipI induces the hypersensitive response (HR), up‐regulating the HR marker gene hin1, in Nicotiana benthamiana. Deletion of R. solanacearum ripI led to increased virulence in tomato (Solanum lycopersicum) plants. Through yeast two‐hybrid and pull‐down assays, we identified an interaction between the N. benthamiana transcription factor bHLH93 and RipI, both of which could be localized in the nucleus of Arabidopsis protoplasts. Silencing of bHLH93 markedly attenuated the RipI‐induced HR and induced expression of the PDF1.2 defence gene. These data demonstrate that the R. solanacearum effector RipI induces a host defence reaction by interacting with the bHLH93 transcription factor. |
format | Online Article Text |
id | pubmed-7279998 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-72799982020-06-10 The Ralstonia solanacearum effector RipI induces a defence reaction by interacting with the bHLH93 transcription factor in Nicotiana benthamiana Zhuo, Tao Wang, Xue Chen, Zhengyu Cui, Haitao Zeng, Yanhong Chen, Yang Fan, Xiaojing Hu, Xun Zou, Huasong Mol Plant Pathol Short Communication Ralstonia solanacearum releases a set of effectors into plant cells that modify the host defence reaction. The role of the effector protein RipI during infection has not been elucidated. In this study, we demonstrated that transient overexpression of RipI induces the hypersensitive response (HR), up‐regulating the HR marker gene hin1, in Nicotiana benthamiana. Deletion of R. solanacearum ripI led to increased virulence in tomato (Solanum lycopersicum) plants. Through yeast two‐hybrid and pull‐down assays, we identified an interaction between the N. benthamiana transcription factor bHLH93 and RipI, both of which could be localized in the nucleus of Arabidopsis protoplasts. Silencing of bHLH93 markedly attenuated the RipI‐induced HR and induced expression of the PDF1.2 defence gene. These data demonstrate that the R. solanacearum effector RipI induces a host defence reaction by interacting with the bHLH93 transcription factor. John Wiley and Sons Inc. 2020-04-13 /pmc/articles/PMC7279998/ /pubmed/32285606 http://dx.doi.org/10.1111/mpp.12937 Text en © 2020 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Short Communication Zhuo, Tao Wang, Xue Chen, Zhengyu Cui, Haitao Zeng, Yanhong Chen, Yang Fan, Xiaojing Hu, Xun Zou, Huasong The Ralstonia solanacearum effector RipI induces a defence reaction by interacting with the bHLH93 transcription factor in Nicotiana benthamiana |
title | The Ralstonia solanacearum effector RipI induces a defence reaction by interacting with the bHLH93 transcription factor in Nicotiana benthamiana
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title_full | The Ralstonia solanacearum effector RipI induces a defence reaction by interacting with the bHLH93 transcription factor in Nicotiana benthamiana
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title_fullStr | The Ralstonia solanacearum effector RipI induces a defence reaction by interacting with the bHLH93 transcription factor in Nicotiana benthamiana
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title_full_unstemmed | The Ralstonia solanacearum effector RipI induces a defence reaction by interacting with the bHLH93 transcription factor in Nicotiana benthamiana
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title_short | The Ralstonia solanacearum effector RipI induces a defence reaction by interacting with the bHLH93 transcription factor in Nicotiana benthamiana
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title_sort | ralstonia solanacearum effector ripi induces a defence reaction by interacting with the bhlh93 transcription factor in nicotiana benthamiana |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7279998/ https://www.ncbi.nlm.nih.gov/pubmed/32285606 http://dx.doi.org/10.1111/mpp.12937 |
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