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Tomato T2 ribonuclease LE is involved in the response to pathogens

T2 ribonucleases (RNases) are RNA‐degrading enzymes that function in various cellular processes, mostly via RNA metabolism. T2 RNase‐encoding genes have been identified in various organisms, from bacteria to mammals, and are most diverse in plants. The existence of T2 RNase genes in almost every org...

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Autores principales: Singh, Naveen Kumar, Paz, Einat, Kutsher, Yaarit, Reuveni, Moshe, Lers, Amnon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7280031/
https://www.ncbi.nlm.nih.gov/pubmed/32352631
http://dx.doi.org/10.1111/mpp.12928
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author Singh, Naveen Kumar
Paz, Einat
Kutsher, Yaarit
Reuveni, Moshe
Lers, Amnon
author_facet Singh, Naveen Kumar
Paz, Einat
Kutsher, Yaarit
Reuveni, Moshe
Lers, Amnon
author_sort Singh, Naveen Kumar
collection PubMed
description T2 ribonucleases (RNases) are RNA‐degrading enzymes that function in various cellular processes, mostly via RNA metabolism. T2 RNase‐encoding genes have been identified in various organisms, from bacteria to mammals, and are most diverse in plants. The existence of T2 RNase genes in almost every organism suggests an important biological function that has been conserved through evolution. In plants, T2 RNases are suggested to be involved in phosphate scavenging and recycling, and are implicated in defence responses to pathogens. We investigated the function of the tomato T2 RNase LE, known to be induced by phosphate deficiency and wounding. The possible involvement of LE in pathogen responses was examined. Expression analysis showed LE induction during fungal infection and by stimuli known to be associated with pathogen inoculation, including oxalic acid and hydrogen peroxide. Analysis of LE‐suppressed transgenic tomato lines revealed higher susceptibility to oxalic acid, a cell death‐inducing factor, compared to the wild type. This elevated sensitivity of LE‐suppressed lines was evidenced by visual signs of necrosis, and increased ion leakage and reactive oxygen species levels, indicating acceleration of cell death. Challenge of the LE‐suppressed lines with the necrotrophic pathogen Botrytis cinerea resulted in accelerated development of disease symptoms compared to the wild type, associated with suppressed expression of pathogenesis‐related marker genes. The results suggest a role for plant endogenous T2 RNases in antifungal activity.
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spelling pubmed-72800312020-06-10 Tomato T2 ribonuclease LE is involved in the response to pathogens Singh, Naveen Kumar Paz, Einat Kutsher, Yaarit Reuveni, Moshe Lers, Amnon Mol Plant Pathol Original Articles T2 ribonucleases (RNases) are RNA‐degrading enzymes that function in various cellular processes, mostly via RNA metabolism. T2 RNase‐encoding genes have been identified in various organisms, from bacteria to mammals, and are most diverse in plants. The existence of T2 RNase genes in almost every organism suggests an important biological function that has been conserved through evolution. In plants, T2 RNases are suggested to be involved in phosphate scavenging and recycling, and are implicated in defence responses to pathogens. We investigated the function of the tomato T2 RNase LE, known to be induced by phosphate deficiency and wounding. The possible involvement of LE in pathogen responses was examined. Expression analysis showed LE induction during fungal infection and by stimuli known to be associated with pathogen inoculation, including oxalic acid and hydrogen peroxide. Analysis of LE‐suppressed transgenic tomato lines revealed higher susceptibility to oxalic acid, a cell death‐inducing factor, compared to the wild type. This elevated sensitivity of LE‐suppressed lines was evidenced by visual signs of necrosis, and increased ion leakage and reactive oxygen species levels, indicating acceleration of cell death. Challenge of the LE‐suppressed lines with the necrotrophic pathogen Botrytis cinerea resulted in accelerated development of disease symptoms compared to the wild type, associated with suppressed expression of pathogenesis‐related marker genes. The results suggest a role for plant endogenous T2 RNases in antifungal activity. John Wiley and Sons Inc. 2020-04-30 /pmc/articles/PMC7280031/ /pubmed/32352631 http://dx.doi.org/10.1111/mpp.12928 Text en © 2020 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Singh, Naveen Kumar
Paz, Einat
Kutsher, Yaarit
Reuveni, Moshe
Lers, Amnon
Tomato T2 ribonuclease LE is involved in the response to pathogens
title Tomato T2 ribonuclease LE is involved in the response to pathogens
title_full Tomato T2 ribonuclease LE is involved in the response to pathogens
title_fullStr Tomato T2 ribonuclease LE is involved in the response to pathogens
title_full_unstemmed Tomato T2 ribonuclease LE is involved in the response to pathogens
title_short Tomato T2 ribonuclease LE is involved in the response to pathogens
title_sort tomato t2 ribonuclease le is involved in the response to pathogens
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7280031/
https://www.ncbi.nlm.nih.gov/pubmed/32352631
http://dx.doi.org/10.1111/mpp.12928
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