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lncRNA MT1JP Suppresses Biological Activities of Breast Cancer Cells in vitro and in vivo by Regulating the miRNA-214/RUNX3 Axis
INTRODUCTION: The purpose of our research was to evaluate MT1JP in breast cancer. MATERIAL AND METHODS: For clinical purpose, tissues were collected, and a correlation analysis ofMT1JP and miRNA-214 gene expressions was conducted. Using an in vitro study, MDA-MB-231 and MCF-7 cell lines were used as...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Dove
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7280253/ https://www.ncbi.nlm.nih.gov/pubmed/32581560 http://dx.doi.org/10.2147/OTT.S241503 |
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author | Ouyang, Qianwen Cui, Yanru Yang, Shixin Wei, Wensong Zhang, Mingyue Zeng, Jie Qu, Fei |
author_facet | Ouyang, Qianwen Cui, Yanru Yang, Shixin Wei, Wensong Zhang, Mingyue Zeng, Jie Qu, Fei |
author_sort | Ouyang, Qianwen |
collection | PubMed |
description | INTRODUCTION: The purpose of our research was to evaluate MT1JP in breast cancer. MATERIAL AND METHODS: For clinical purpose, tissues were collected, and a correlation analysis ofMT1JP and miRNA-214 gene expressions was conducted. Using an in vitro study, MDA-MB-231 and MCF-7 cell lines were used as research objects in our research. Colony, flow cytometry, TUNEL, transwell, adhesion and wound healing assay were used to discuss the biological activities of the cells. In an in vivo study, tumor weight and volume were measured, and cell apoptosis was measured by TUNEL assay. The relative mechanism’s proteins were evaluated by Western blotting or immunohistochemistry assay. RESULTS: Compared with adjacent tissues, MT1JP and miRNA-214 gene expressions were significantly different (P<0.001, respectively). By in vitro and in vivo studies, the biological activities of the cells were significantly decreased in MDA-MB-231 and MCF-7 cell lines with MT1JP overexpression. The relative mechanism was correlated with miRNA-214/RUNX3 axis. CONCLUSION: The overexpression of MT1JP suppresses the biological activities of breast cancer cells by regulation miRNA-214/RUNX3 axis in vitro and vivo study. |
format | Online Article Text |
id | pubmed-7280253 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-72802532020-06-23 lncRNA MT1JP Suppresses Biological Activities of Breast Cancer Cells in vitro and in vivo by Regulating the miRNA-214/RUNX3 Axis Ouyang, Qianwen Cui, Yanru Yang, Shixin Wei, Wensong Zhang, Mingyue Zeng, Jie Qu, Fei Onco Targets Ther Original Research INTRODUCTION: The purpose of our research was to evaluate MT1JP in breast cancer. MATERIAL AND METHODS: For clinical purpose, tissues were collected, and a correlation analysis ofMT1JP and miRNA-214 gene expressions was conducted. Using an in vitro study, MDA-MB-231 and MCF-7 cell lines were used as research objects in our research. Colony, flow cytometry, TUNEL, transwell, adhesion and wound healing assay were used to discuss the biological activities of the cells. In an in vivo study, tumor weight and volume were measured, and cell apoptosis was measured by TUNEL assay. The relative mechanism’s proteins were evaluated by Western blotting or immunohistochemistry assay. RESULTS: Compared with adjacent tissues, MT1JP and miRNA-214 gene expressions were significantly different (P<0.001, respectively). By in vitro and in vivo studies, the biological activities of the cells were significantly decreased in MDA-MB-231 and MCF-7 cell lines with MT1JP overexpression. The relative mechanism was correlated with miRNA-214/RUNX3 axis. CONCLUSION: The overexpression of MT1JP suppresses the biological activities of breast cancer cells by regulation miRNA-214/RUNX3 axis in vitro and vivo study. Dove 2020-06-04 /pmc/articles/PMC7280253/ /pubmed/32581560 http://dx.doi.org/10.2147/OTT.S241503 Text en © 2020 Ouyang et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Ouyang, Qianwen Cui, Yanru Yang, Shixin Wei, Wensong Zhang, Mingyue Zeng, Jie Qu, Fei lncRNA MT1JP Suppresses Biological Activities of Breast Cancer Cells in vitro and in vivo by Regulating the miRNA-214/RUNX3 Axis |
title | lncRNA MT1JP Suppresses Biological Activities of Breast Cancer Cells in vitro and in vivo by Regulating the miRNA-214/RUNX3 Axis |
title_full | lncRNA MT1JP Suppresses Biological Activities of Breast Cancer Cells in vitro and in vivo by Regulating the miRNA-214/RUNX3 Axis |
title_fullStr | lncRNA MT1JP Suppresses Biological Activities of Breast Cancer Cells in vitro and in vivo by Regulating the miRNA-214/RUNX3 Axis |
title_full_unstemmed | lncRNA MT1JP Suppresses Biological Activities of Breast Cancer Cells in vitro and in vivo by Regulating the miRNA-214/RUNX3 Axis |
title_short | lncRNA MT1JP Suppresses Biological Activities of Breast Cancer Cells in vitro and in vivo by Regulating the miRNA-214/RUNX3 Axis |
title_sort | lncrna mt1jp suppresses biological activities of breast cancer cells in vitro and in vivo by regulating the mirna-214/runx3 axis |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7280253/ https://www.ncbi.nlm.nih.gov/pubmed/32581560 http://dx.doi.org/10.2147/OTT.S241503 |
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