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Protection and immune modulation of activated human vaginal epithelial cells by Aurea helianthus extract
Aurea helianthus extract is associated with various properties including anti-melanogenesis, anti-oxidation, tumorigenic suppression, and immunoregulation; however, the mechanism by which it executes the immunomodulation of human vaginal epithelial cells (HVECs) remains elusive. We established three...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7280265/ https://www.ncbi.nlm.nih.gov/pubmed/32513981 http://dx.doi.org/10.1038/s41598-020-65821-4 |
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author | Park, Yoonjin Lee, Kyunghwa Lee, Chayul Song, Ahran Kim, Jinkwan Kim, Boyong Lee, SeungGwan |
author_facet | Park, Yoonjin Lee, Kyunghwa Lee, Chayul Song, Ahran Kim, Jinkwan Kim, Boyong Lee, SeungGwan |
author_sort | Park, Yoonjin |
collection | PubMed |
description | Aurea helianthus extract is associated with various properties including anti-melanogenesis, anti-oxidation, tumorigenic suppression, and immunoregulation; however, the mechanism by which it executes the immunomodulation of human vaginal epithelial cells (HVECs) remains elusive. We established three immunological functions of the extract. First, it mediated tumorigenic suppression in HVECs. Expression of cytokeratin 8, cancer antigen-125, and vimentin was dramatically downregulated in HVECs exposed to the extract under oxidative and fungal stresses. Second, the extract activated dendritic cells and macrophages. On exposing progenitor dendritic cells to the extract, the number of CD304(+) cells increased by 40%; further, under oxidative and fungal stresses, this number was approximately 1.8 and 1.3 times lower, respectively, compared to that in the stressed cells. In monocytic differentiation, the number of dendritic cells and macrophages increased 9 and 6 times, respectively, compared to that in the control. Additionally, the extract enhanced and recovered polarisation by approximately 1.5 and 2 times, respectively, than that under stressed conditions. Third, the phagocytic activity of macrophages, against HPV16, 18, and 33 peptides, was enhanced by 12–35 times compared with that under stressed conditions. Thus, A. helianthus extract is a strong stimulator of the immune system and tumorigenic suppression under stress conditions. |
format | Online Article Text |
id | pubmed-7280265 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-72802652020-06-15 Protection and immune modulation of activated human vaginal epithelial cells by Aurea helianthus extract Park, Yoonjin Lee, Kyunghwa Lee, Chayul Song, Ahran Kim, Jinkwan Kim, Boyong Lee, SeungGwan Sci Rep Article Aurea helianthus extract is associated with various properties including anti-melanogenesis, anti-oxidation, tumorigenic suppression, and immunoregulation; however, the mechanism by which it executes the immunomodulation of human vaginal epithelial cells (HVECs) remains elusive. We established three immunological functions of the extract. First, it mediated tumorigenic suppression in HVECs. Expression of cytokeratin 8, cancer antigen-125, and vimentin was dramatically downregulated in HVECs exposed to the extract under oxidative and fungal stresses. Second, the extract activated dendritic cells and macrophages. On exposing progenitor dendritic cells to the extract, the number of CD304(+) cells increased by 40%; further, under oxidative and fungal stresses, this number was approximately 1.8 and 1.3 times lower, respectively, compared to that in the stressed cells. In monocytic differentiation, the number of dendritic cells and macrophages increased 9 and 6 times, respectively, compared to that in the control. Additionally, the extract enhanced and recovered polarisation by approximately 1.5 and 2 times, respectively, than that under stressed conditions. Third, the phagocytic activity of macrophages, against HPV16, 18, and 33 peptides, was enhanced by 12–35 times compared with that under stressed conditions. Thus, A. helianthus extract is a strong stimulator of the immune system and tumorigenic suppression under stress conditions. Nature Publishing Group UK 2020-06-08 /pmc/articles/PMC7280265/ /pubmed/32513981 http://dx.doi.org/10.1038/s41598-020-65821-4 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Park, Yoonjin Lee, Kyunghwa Lee, Chayul Song, Ahran Kim, Jinkwan Kim, Boyong Lee, SeungGwan Protection and immune modulation of activated human vaginal epithelial cells by Aurea helianthus extract |
title | Protection and immune modulation of activated human vaginal epithelial cells by Aurea helianthus extract |
title_full | Protection and immune modulation of activated human vaginal epithelial cells by Aurea helianthus extract |
title_fullStr | Protection and immune modulation of activated human vaginal epithelial cells by Aurea helianthus extract |
title_full_unstemmed | Protection and immune modulation of activated human vaginal epithelial cells by Aurea helianthus extract |
title_short | Protection and immune modulation of activated human vaginal epithelial cells by Aurea helianthus extract |
title_sort | protection and immune modulation of activated human vaginal epithelial cells by aurea helianthus extract |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7280265/ https://www.ncbi.nlm.nih.gov/pubmed/32513981 http://dx.doi.org/10.1038/s41598-020-65821-4 |
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