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Characterizing Peri-Implant and Sub-Gingival Microbiota through Culturomics. First Isolation of Some Species in the Oral Cavity. A Pilot Study

Background: In recent years, culture-independent molecular techniques have been developed to investigate microbiota considered uncultivable. However, the data in the literature suggest that molecular techniques and cultural methods target different spectra of bacteria. The objective of this pilot st...

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Autores principales: Martellacci, Leonardo, Quaranta, Gianluca, Fancello, Giovanni, D’Addona, Antonio, Sanguinetti, Maurizio, Patini, Romeo, Masucci, Luca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7280956/
https://www.ncbi.nlm.nih.gov/pubmed/32397631
http://dx.doi.org/10.3390/pathogens9050365
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author Martellacci, Leonardo
Quaranta, Gianluca
Fancello, Giovanni
D’Addona, Antonio
Sanguinetti, Maurizio
Patini, Romeo
Masucci, Luca
author_facet Martellacci, Leonardo
Quaranta, Gianluca
Fancello, Giovanni
D’Addona, Antonio
Sanguinetti, Maurizio
Patini, Romeo
Masucci, Luca
author_sort Martellacci, Leonardo
collection PubMed
description Background: In recent years, culture-independent molecular techniques have been developed to investigate microbiota considered uncultivable. However, the data in the literature suggest that molecular techniques and cultural methods target different spectra of bacteria. The objective of this pilot study was to search for not yet identified oral species in the peri-implant and sub-gingival microbiota in patients without signs of oral pathologies, through the use of the culturomics approach, which has never been used before in dentistry. Methods: Four patients were enrolled; from each patient, samples of sub-gingival and peri-implant plaque were taken and analysed by culturomics. Results: Of 48 isolated species, only 30 had been previously identified by metagenomics in other studies; on the contrary, 12 species had never been associated with the oral cavity before, and 5 of them had never been isolated from clinical specimens. Conclusions: By adopting culturomics in dentistry, it could be possible to identify a large amount of fastidious microorganisms that inhabit the oral cavity and to more accurately characterize the microorganisms that lead to periodontitis and peri-implantitis. This evidence could represent an important step forward for the diagnosis and treatment of peri-implantitis, as well as a very useful means for the characterization of new potential aetiologic agents.
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spelling pubmed-72809562020-06-15 Characterizing Peri-Implant and Sub-Gingival Microbiota through Culturomics. First Isolation of Some Species in the Oral Cavity. A Pilot Study Martellacci, Leonardo Quaranta, Gianluca Fancello, Giovanni D’Addona, Antonio Sanguinetti, Maurizio Patini, Romeo Masucci, Luca Pathogens Article Background: In recent years, culture-independent molecular techniques have been developed to investigate microbiota considered uncultivable. However, the data in the literature suggest that molecular techniques and cultural methods target different spectra of bacteria. The objective of this pilot study was to search for not yet identified oral species in the peri-implant and sub-gingival microbiota in patients without signs of oral pathologies, through the use of the culturomics approach, which has never been used before in dentistry. Methods: Four patients were enrolled; from each patient, samples of sub-gingival and peri-implant plaque were taken and analysed by culturomics. Results: Of 48 isolated species, only 30 had been previously identified by metagenomics in other studies; on the contrary, 12 species had never been associated with the oral cavity before, and 5 of them had never been isolated from clinical specimens. Conclusions: By adopting culturomics in dentistry, it could be possible to identify a large amount of fastidious microorganisms that inhabit the oral cavity and to more accurately characterize the microorganisms that lead to periodontitis and peri-implantitis. This evidence could represent an important step forward for the diagnosis and treatment of peri-implantitis, as well as a very useful means for the characterization of new potential aetiologic agents. MDPI 2020-05-10 /pmc/articles/PMC7280956/ /pubmed/32397631 http://dx.doi.org/10.3390/pathogens9050365 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Martellacci, Leonardo
Quaranta, Gianluca
Fancello, Giovanni
D’Addona, Antonio
Sanguinetti, Maurizio
Patini, Romeo
Masucci, Luca
Characterizing Peri-Implant and Sub-Gingival Microbiota through Culturomics. First Isolation of Some Species in the Oral Cavity. A Pilot Study
title Characterizing Peri-Implant and Sub-Gingival Microbiota through Culturomics. First Isolation of Some Species in the Oral Cavity. A Pilot Study
title_full Characterizing Peri-Implant and Sub-Gingival Microbiota through Culturomics. First Isolation of Some Species in the Oral Cavity. A Pilot Study
title_fullStr Characterizing Peri-Implant and Sub-Gingival Microbiota through Culturomics. First Isolation of Some Species in the Oral Cavity. A Pilot Study
title_full_unstemmed Characterizing Peri-Implant and Sub-Gingival Microbiota through Culturomics. First Isolation of Some Species in the Oral Cavity. A Pilot Study
title_short Characterizing Peri-Implant and Sub-Gingival Microbiota through Culturomics. First Isolation of Some Species in the Oral Cavity. A Pilot Study
title_sort characterizing peri-implant and sub-gingival microbiota through culturomics. first isolation of some species in the oral cavity. a pilot study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7280956/
https://www.ncbi.nlm.nih.gov/pubmed/32397631
http://dx.doi.org/10.3390/pathogens9050365
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