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Frequency of Resistance to Benzimidazoles of Haemonchus contortus Helminths from Dairy Sheep, Goats, Cattle and Buffaloes in Greece

The study investigated the presence of resistance to benzimidazoles in Haemonchus contortus helminths from ruminant species in Greece through the detection of the Phe/Tyr polymorphism in the amino acid at position 200 of the β-tubulin protein. In total, 288 adult female H. contortus helminths collec...

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Autores principales: Arsenopoulos, Konstantinos, Minoudi, Styliani, Symeonidou, Isaia, Triantafyllidis, Alexandros, Katsafadou, Angeliki I., Lianou, Daphne T., Fthenakis, George C., Papadopoulos, Elias
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7280990/
https://www.ncbi.nlm.nih.gov/pubmed/32375252
http://dx.doi.org/10.3390/pathogens9050347
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author Arsenopoulos, Konstantinos
Minoudi, Styliani
Symeonidou, Isaia
Triantafyllidis, Alexandros
Katsafadou, Angeliki I.
Lianou, Daphne T.
Fthenakis, George C.
Papadopoulos, Elias
author_facet Arsenopoulos, Konstantinos
Minoudi, Styliani
Symeonidou, Isaia
Triantafyllidis, Alexandros
Katsafadou, Angeliki I.
Lianou, Daphne T.
Fthenakis, George C.
Papadopoulos, Elias
author_sort Arsenopoulos, Konstantinos
collection PubMed
description The study investigated the presence of resistance to benzimidazoles in Haemonchus contortus helminths from ruminant species in Greece through the detection of the Phe/Tyr polymorphism in the amino acid at position 200 of the β-tubulin protein. In total, 288 adult female H. contortus helminths collected from the abomasum of various ruminant animals in Greece were tested. Of these, 96 were collected from sheep, 96 from goats, 48 from cattle, and 48 from buffaloes. The frequencies of the homozygous and heterozygous resistant genotypes at the position 200 of the β-tubulin gene of helminths recovered from sheep were 96.9% and 3.1%, respectively. The frequencies of the homozygous and heterozygous resistant genotypes, respectively, were 100.0% and 0.0% in helminths from goats, 25.0% and 75.0% in helminths from cattle and 8.3% and 91.7% in helminths from buffaloes. In all parasitic populations, no homozygous susceptible genotypes were detected. The present study highlighted, for the first time, the emergence of benzimidazole-resistant H. contortus in goats, cattle, and buffaloes in Greece, using an allele-specific PCR. It is postulated that benzimidazole-resistant alleles were transferred from sheep or goats to cattle and buffaloes at the commonly grazing pastures in Greece.
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spelling pubmed-72809902020-06-15 Frequency of Resistance to Benzimidazoles of Haemonchus contortus Helminths from Dairy Sheep, Goats, Cattle and Buffaloes in Greece Arsenopoulos, Konstantinos Minoudi, Styliani Symeonidou, Isaia Triantafyllidis, Alexandros Katsafadou, Angeliki I. Lianou, Daphne T. Fthenakis, George C. Papadopoulos, Elias Pathogens Article The study investigated the presence of resistance to benzimidazoles in Haemonchus contortus helminths from ruminant species in Greece through the detection of the Phe/Tyr polymorphism in the amino acid at position 200 of the β-tubulin protein. In total, 288 adult female H. contortus helminths collected from the abomasum of various ruminant animals in Greece were tested. Of these, 96 were collected from sheep, 96 from goats, 48 from cattle, and 48 from buffaloes. The frequencies of the homozygous and heterozygous resistant genotypes at the position 200 of the β-tubulin gene of helminths recovered from sheep were 96.9% and 3.1%, respectively. The frequencies of the homozygous and heterozygous resistant genotypes, respectively, were 100.0% and 0.0% in helminths from goats, 25.0% and 75.0% in helminths from cattle and 8.3% and 91.7% in helminths from buffaloes. In all parasitic populations, no homozygous susceptible genotypes were detected. The present study highlighted, for the first time, the emergence of benzimidazole-resistant H. contortus in goats, cattle, and buffaloes in Greece, using an allele-specific PCR. It is postulated that benzimidazole-resistant alleles were transferred from sheep or goats to cattle and buffaloes at the commonly grazing pastures in Greece. MDPI 2020-05-03 /pmc/articles/PMC7280990/ /pubmed/32375252 http://dx.doi.org/10.3390/pathogens9050347 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Arsenopoulos, Konstantinos
Minoudi, Styliani
Symeonidou, Isaia
Triantafyllidis, Alexandros
Katsafadou, Angeliki I.
Lianou, Daphne T.
Fthenakis, George C.
Papadopoulos, Elias
Frequency of Resistance to Benzimidazoles of Haemonchus contortus Helminths from Dairy Sheep, Goats, Cattle and Buffaloes in Greece
title Frequency of Resistance to Benzimidazoles of Haemonchus contortus Helminths from Dairy Sheep, Goats, Cattle and Buffaloes in Greece
title_full Frequency of Resistance to Benzimidazoles of Haemonchus contortus Helminths from Dairy Sheep, Goats, Cattle and Buffaloes in Greece
title_fullStr Frequency of Resistance to Benzimidazoles of Haemonchus contortus Helminths from Dairy Sheep, Goats, Cattle and Buffaloes in Greece
title_full_unstemmed Frequency of Resistance to Benzimidazoles of Haemonchus contortus Helminths from Dairy Sheep, Goats, Cattle and Buffaloes in Greece
title_short Frequency of Resistance to Benzimidazoles of Haemonchus contortus Helminths from Dairy Sheep, Goats, Cattle and Buffaloes in Greece
title_sort frequency of resistance to benzimidazoles of haemonchus contortus helminths from dairy sheep, goats, cattle and buffaloes in greece
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7280990/
https://www.ncbi.nlm.nih.gov/pubmed/32375252
http://dx.doi.org/10.3390/pathogens9050347
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