Cargando…

Chitosan-Gentamicin Conjugate Hydrogel Promoting Skin Scald Repair

Our earlier research indicated that chitosan-gentamicin conjugate (CS-GT) possesses superior antimicrobial activity and good water solubility. To develop CS-GT-based scald dressings, the antibacterial properties of CS-GT were further studied, and the biosafety of CS-GT and the healing mechanism of C...

Descripción completa

Detalles Bibliográficos
Autores principales: Yan, Tingting, Kong, Songzhi, Ouyang, Qianqian, Li, Chengpeng, Hou, Tingting, Chen, Yu, Li, Sidong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281128/
https://www.ncbi.nlm.nih.gov/pubmed/32365733
http://dx.doi.org/10.3390/md18050233
_version_ 1783543850280681472
author Yan, Tingting
Kong, Songzhi
Ouyang, Qianqian
Li, Chengpeng
Hou, Tingting
Chen, Yu
Li, Sidong
author_facet Yan, Tingting
Kong, Songzhi
Ouyang, Qianqian
Li, Chengpeng
Hou, Tingting
Chen, Yu
Li, Sidong
author_sort Yan, Tingting
collection PubMed
description Our earlier research indicated that chitosan-gentamicin conjugate (CS-GT) possesses superior antimicrobial activity and good water solubility. To develop CS-GT-based scald dressings, the antibacterial properties of CS-GT were further studied, and the biosafety of CS-GT and the healing mechanism of CS-GT hydrogel was systematically explored in this article. It was found that cell viability shows a declined inclination with the prolonged culture time and the increased concentration of CS-GT. After three day’s culture, the cell viability could still remain at 79.72% when CS-GT concentration was as high as 1000 μg/mL. On the other hand, the hemolysis rate of CS-GT was lower than 5% when its concentration is 800 μg/mL. Therefore CS-GT has good cytocompatibility and hemocompatibility. A wound-healing experiment has shown that the skin healing rate of CS-GT hydrogel was the highest at 99.61%, followed by the positive control (wet burn ointment) 94.98%, GT hydrogel 87.50%, and matrix 77.39%. The blank control group, however, possessed the lowest healing rate of 75.45%. Further analysis indicated that CS-GT hydrogel could promote the synthesis of total protein (TP) in skin granulation tissue, resulting in the enhanced hydroxyproline (HYP) content, which facilitated collagen fibrogenesis, reduced cytokine expression in an inflammatory response, and, ultimately, accelerated wound healing. To sum up, CS-GT hydrogel is a promising scald dressing.
format Online
Article
Text
id pubmed-7281128
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-72811282020-06-15 Chitosan-Gentamicin Conjugate Hydrogel Promoting Skin Scald Repair Yan, Tingting Kong, Songzhi Ouyang, Qianqian Li, Chengpeng Hou, Tingting Chen, Yu Li, Sidong Mar Drugs Article Our earlier research indicated that chitosan-gentamicin conjugate (CS-GT) possesses superior antimicrobial activity and good water solubility. To develop CS-GT-based scald dressings, the antibacterial properties of CS-GT were further studied, and the biosafety of CS-GT and the healing mechanism of CS-GT hydrogel was systematically explored in this article. It was found that cell viability shows a declined inclination with the prolonged culture time and the increased concentration of CS-GT. After three day’s culture, the cell viability could still remain at 79.72% when CS-GT concentration was as high as 1000 μg/mL. On the other hand, the hemolysis rate of CS-GT was lower than 5% when its concentration is 800 μg/mL. Therefore CS-GT has good cytocompatibility and hemocompatibility. A wound-healing experiment has shown that the skin healing rate of CS-GT hydrogel was the highest at 99.61%, followed by the positive control (wet burn ointment) 94.98%, GT hydrogel 87.50%, and matrix 77.39%. The blank control group, however, possessed the lowest healing rate of 75.45%. Further analysis indicated that CS-GT hydrogel could promote the synthesis of total protein (TP) in skin granulation tissue, resulting in the enhanced hydroxyproline (HYP) content, which facilitated collagen fibrogenesis, reduced cytokine expression in an inflammatory response, and, ultimately, accelerated wound healing. To sum up, CS-GT hydrogel is a promising scald dressing. MDPI 2020-04-29 /pmc/articles/PMC7281128/ /pubmed/32365733 http://dx.doi.org/10.3390/md18050233 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yan, Tingting
Kong, Songzhi
Ouyang, Qianqian
Li, Chengpeng
Hou, Tingting
Chen, Yu
Li, Sidong
Chitosan-Gentamicin Conjugate Hydrogel Promoting Skin Scald Repair
title Chitosan-Gentamicin Conjugate Hydrogel Promoting Skin Scald Repair
title_full Chitosan-Gentamicin Conjugate Hydrogel Promoting Skin Scald Repair
title_fullStr Chitosan-Gentamicin Conjugate Hydrogel Promoting Skin Scald Repair
title_full_unstemmed Chitosan-Gentamicin Conjugate Hydrogel Promoting Skin Scald Repair
title_short Chitosan-Gentamicin Conjugate Hydrogel Promoting Skin Scald Repair
title_sort chitosan-gentamicin conjugate hydrogel promoting skin scald repair
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281128/
https://www.ncbi.nlm.nih.gov/pubmed/32365733
http://dx.doi.org/10.3390/md18050233
work_keys_str_mv AT yantingting chitosangentamicinconjugatehydrogelpromotingskinscaldrepair
AT kongsongzhi chitosangentamicinconjugatehydrogelpromotingskinscaldrepair
AT ouyangqianqian chitosangentamicinconjugatehydrogelpromotingskinscaldrepair
AT lichengpeng chitosangentamicinconjugatehydrogelpromotingskinscaldrepair
AT houtingting chitosangentamicinconjugatehydrogelpromotingskinscaldrepair
AT chenyu chitosangentamicinconjugatehydrogelpromotingskinscaldrepair
AT lisidong chitosangentamicinconjugatehydrogelpromotingskinscaldrepair