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Chitosan-Gentamicin Conjugate Hydrogel Promoting Skin Scald Repair
Our earlier research indicated that chitosan-gentamicin conjugate (CS-GT) possesses superior antimicrobial activity and good water solubility. To develop CS-GT-based scald dressings, the antibacterial properties of CS-GT were further studied, and the biosafety of CS-GT and the healing mechanism of C...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281128/ https://www.ncbi.nlm.nih.gov/pubmed/32365733 http://dx.doi.org/10.3390/md18050233 |
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author | Yan, Tingting Kong, Songzhi Ouyang, Qianqian Li, Chengpeng Hou, Tingting Chen, Yu Li, Sidong |
author_facet | Yan, Tingting Kong, Songzhi Ouyang, Qianqian Li, Chengpeng Hou, Tingting Chen, Yu Li, Sidong |
author_sort | Yan, Tingting |
collection | PubMed |
description | Our earlier research indicated that chitosan-gentamicin conjugate (CS-GT) possesses superior antimicrobial activity and good water solubility. To develop CS-GT-based scald dressings, the antibacterial properties of CS-GT were further studied, and the biosafety of CS-GT and the healing mechanism of CS-GT hydrogel was systematically explored in this article. It was found that cell viability shows a declined inclination with the prolonged culture time and the increased concentration of CS-GT. After three day’s culture, the cell viability could still remain at 79.72% when CS-GT concentration was as high as 1000 μg/mL. On the other hand, the hemolysis rate of CS-GT was lower than 5% when its concentration is 800 μg/mL. Therefore CS-GT has good cytocompatibility and hemocompatibility. A wound-healing experiment has shown that the skin healing rate of CS-GT hydrogel was the highest at 99.61%, followed by the positive control (wet burn ointment) 94.98%, GT hydrogel 87.50%, and matrix 77.39%. The blank control group, however, possessed the lowest healing rate of 75.45%. Further analysis indicated that CS-GT hydrogel could promote the synthesis of total protein (TP) in skin granulation tissue, resulting in the enhanced hydroxyproline (HYP) content, which facilitated collagen fibrogenesis, reduced cytokine expression in an inflammatory response, and, ultimately, accelerated wound healing. To sum up, CS-GT hydrogel is a promising scald dressing. |
format | Online Article Text |
id | pubmed-7281128 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72811282020-06-15 Chitosan-Gentamicin Conjugate Hydrogel Promoting Skin Scald Repair Yan, Tingting Kong, Songzhi Ouyang, Qianqian Li, Chengpeng Hou, Tingting Chen, Yu Li, Sidong Mar Drugs Article Our earlier research indicated that chitosan-gentamicin conjugate (CS-GT) possesses superior antimicrobial activity and good water solubility. To develop CS-GT-based scald dressings, the antibacterial properties of CS-GT were further studied, and the biosafety of CS-GT and the healing mechanism of CS-GT hydrogel was systematically explored in this article. It was found that cell viability shows a declined inclination with the prolonged culture time and the increased concentration of CS-GT. After three day’s culture, the cell viability could still remain at 79.72% when CS-GT concentration was as high as 1000 μg/mL. On the other hand, the hemolysis rate of CS-GT was lower than 5% when its concentration is 800 μg/mL. Therefore CS-GT has good cytocompatibility and hemocompatibility. A wound-healing experiment has shown that the skin healing rate of CS-GT hydrogel was the highest at 99.61%, followed by the positive control (wet burn ointment) 94.98%, GT hydrogel 87.50%, and matrix 77.39%. The blank control group, however, possessed the lowest healing rate of 75.45%. Further analysis indicated that CS-GT hydrogel could promote the synthesis of total protein (TP) in skin granulation tissue, resulting in the enhanced hydroxyproline (HYP) content, which facilitated collagen fibrogenesis, reduced cytokine expression in an inflammatory response, and, ultimately, accelerated wound healing. To sum up, CS-GT hydrogel is a promising scald dressing. MDPI 2020-04-29 /pmc/articles/PMC7281128/ /pubmed/32365733 http://dx.doi.org/10.3390/md18050233 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yan, Tingting Kong, Songzhi Ouyang, Qianqian Li, Chengpeng Hou, Tingting Chen, Yu Li, Sidong Chitosan-Gentamicin Conjugate Hydrogel Promoting Skin Scald Repair |
title | Chitosan-Gentamicin Conjugate Hydrogel Promoting Skin Scald Repair |
title_full | Chitosan-Gentamicin Conjugate Hydrogel Promoting Skin Scald Repair |
title_fullStr | Chitosan-Gentamicin Conjugate Hydrogel Promoting Skin Scald Repair |
title_full_unstemmed | Chitosan-Gentamicin Conjugate Hydrogel Promoting Skin Scald Repair |
title_short | Chitosan-Gentamicin Conjugate Hydrogel Promoting Skin Scald Repair |
title_sort | chitosan-gentamicin conjugate hydrogel promoting skin scald repair |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281128/ https://www.ncbi.nlm.nih.gov/pubmed/32365733 http://dx.doi.org/10.3390/md18050233 |
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