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Anti-Influenza A Virus Activity of Rhamnan Sulfate from Green Algae Monostroma nitidum in Mice with Normal and Compromised Immunity

Influenza viruses cause a significant public health burden each year despite the availability of anti-influenza drugs and vaccines. Therefore, new anti-influenza virus agents are needed. Rhamnan sulfate (RS) is a sulfated polysaccharide derived from the green alga Monostroma nitidum. Here, we aimed...

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Autores principales: Terasawa, Masahiro, Hayashi, Kyoko, Lee, Jung-Bum, Nishiura, Kaoru, Matsuda, Koichi, Hayashi, Toshimitsu, Kawahara, Toshio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281209/
https://www.ncbi.nlm.nih.gov/pubmed/32414158
http://dx.doi.org/10.3390/md18050254
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author Terasawa, Masahiro
Hayashi, Kyoko
Lee, Jung-Bum
Nishiura, Kaoru
Matsuda, Koichi
Hayashi, Toshimitsu
Kawahara, Toshio
author_facet Terasawa, Masahiro
Hayashi, Kyoko
Lee, Jung-Bum
Nishiura, Kaoru
Matsuda, Koichi
Hayashi, Toshimitsu
Kawahara, Toshio
author_sort Terasawa, Masahiro
collection PubMed
description Influenza viruses cause a significant public health burden each year despite the availability of anti-influenza drugs and vaccines. Therefore, new anti-influenza virus agents are needed. Rhamnan sulfate (RS) is a sulfated polysaccharide derived from the green alga Monostroma nitidum. Here, we aimed to demonstrate the antiviral activity of RS, especially against influenza A virus (IFV) infection, in vitro and in vivo. RS showed inhibitory effects on viral proliferation of enveloped viruses in vitro. Evaluation of the anti-IFV activity of RS in vitro showed that it inhibited both virus adsorption and entry steps. The oral administration of RS in IFV-infected immunocompetent and immunocompromised mice suppressed viral proliferation in both mouse types. The oral administration of RS also had stimulatory effects on neutralizing antibody production. Fluorescent analysis showed that RS colocalized with M cells in Peyer’s patches, suggesting that RS bound to the M cells and may be incorporated into the Peyer’s patches, which are essential to intestinal immunity. In summary, RS inhibits influenza virus infection and promotes antibody production, suggesting that RS is a potential candidate for the treatment of influenza virus infections.
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spelling pubmed-72812092020-06-15 Anti-Influenza A Virus Activity of Rhamnan Sulfate from Green Algae Monostroma nitidum in Mice with Normal and Compromised Immunity Terasawa, Masahiro Hayashi, Kyoko Lee, Jung-Bum Nishiura, Kaoru Matsuda, Koichi Hayashi, Toshimitsu Kawahara, Toshio Mar Drugs Article Influenza viruses cause a significant public health burden each year despite the availability of anti-influenza drugs and vaccines. Therefore, new anti-influenza virus agents are needed. Rhamnan sulfate (RS) is a sulfated polysaccharide derived from the green alga Monostroma nitidum. Here, we aimed to demonstrate the antiviral activity of RS, especially against influenza A virus (IFV) infection, in vitro and in vivo. RS showed inhibitory effects on viral proliferation of enveloped viruses in vitro. Evaluation of the anti-IFV activity of RS in vitro showed that it inhibited both virus adsorption and entry steps. The oral administration of RS in IFV-infected immunocompetent and immunocompromised mice suppressed viral proliferation in both mouse types. The oral administration of RS also had stimulatory effects on neutralizing antibody production. Fluorescent analysis showed that RS colocalized with M cells in Peyer’s patches, suggesting that RS bound to the M cells and may be incorporated into the Peyer’s patches, which are essential to intestinal immunity. In summary, RS inhibits influenza virus infection and promotes antibody production, suggesting that RS is a potential candidate for the treatment of influenza virus infections. MDPI 2020-05-13 /pmc/articles/PMC7281209/ /pubmed/32414158 http://dx.doi.org/10.3390/md18050254 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Terasawa, Masahiro
Hayashi, Kyoko
Lee, Jung-Bum
Nishiura, Kaoru
Matsuda, Koichi
Hayashi, Toshimitsu
Kawahara, Toshio
Anti-Influenza A Virus Activity of Rhamnan Sulfate from Green Algae Monostroma nitidum in Mice with Normal and Compromised Immunity
title Anti-Influenza A Virus Activity of Rhamnan Sulfate from Green Algae Monostroma nitidum in Mice with Normal and Compromised Immunity
title_full Anti-Influenza A Virus Activity of Rhamnan Sulfate from Green Algae Monostroma nitidum in Mice with Normal and Compromised Immunity
title_fullStr Anti-Influenza A Virus Activity of Rhamnan Sulfate from Green Algae Monostroma nitidum in Mice with Normal and Compromised Immunity
title_full_unstemmed Anti-Influenza A Virus Activity of Rhamnan Sulfate from Green Algae Monostroma nitidum in Mice with Normal and Compromised Immunity
title_short Anti-Influenza A Virus Activity of Rhamnan Sulfate from Green Algae Monostroma nitidum in Mice with Normal and Compromised Immunity
title_sort anti-influenza a virus activity of rhamnan sulfate from green algae monostroma nitidum in mice with normal and compromised immunity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281209/
https://www.ncbi.nlm.nih.gov/pubmed/32414158
http://dx.doi.org/10.3390/md18050254
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