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Passive Dielectrophoretic Focusing of Particles and Cells in Ratchet Microchannels

Focusing particles into a tight stream is critical for many microfluidic particle-handling devices such as flow cytometers and particle sorters. This work presents a fundamental study of the passive focusing of polystyrene particles in ratchet microchannels via direct current dielectrophoresis (DC D...

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Autores principales: Lu, Song-Yu, Malekanfard, Amirreza, Beladi-Behbahani, Shayesteh, Zu, Wuzhou, Kale, Akshay, Tzeng, Tzuen-Rong, Wang, Yao-Nan, Xuan, Xiangchun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281238/
https://www.ncbi.nlm.nih.gov/pubmed/32344887
http://dx.doi.org/10.3390/mi11050451
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author Lu, Song-Yu
Malekanfard, Amirreza
Beladi-Behbahani, Shayesteh
Zu, Wuzhou
Kale, Akshay
Tzeng, Tzuen-Rong
Wang, Yao-Nan
Xuan, Xiangchun
author_facet Lu, Song-Yu
Malekanfard, Amirreza
Beladi-Behbahani, Shayesteh
Zu, Wuzhou
Kale, Akshay
Tzeng, Tzuen-Rong
Wang, Yao-Nan
Xuan, Xiangchun
author_sort Lu, Song-Yu
collection PubMed
description Focusing particles into a tight stream is critical for many microfluidic particle-handling devices such as flow cytometers and particle sorters. This work presents a fundamental study of the passive focusing of polystyrene particles in ratchet microchannels via direct current dielectrophoresis (DC DEP). We demonstrate using both experiments and simulation that particles achieve better focusing in a symmetric ratchet microchannel than in an asymmetric one, regardless of the particle movement direction in the latter. The particle focusing ratio, which is defined as the microchannel width over the particle stream width, is found to increase with an increase in particle size or electric field in the symmetric ratchet microchannel. Moreover, it exhibits an almost linear correlation with the number of ratchets, which can be explained by a theoretical formula that is obtained from a scaling analysis. In addition, we have demonstrated a DC dielectrophoretic focusing of yeast cells in the symmetric ratchet microchannel with minimal impact on the cell viability.
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spelling pubmed-72812382020-06-15 Passive Dielectrophoretic Focusing of Particles and Cells in Ratchet Microchannels Lu, Song-Yu Malekanfard, Amirreza Beladi-Behbahani, Shayesteh Zu, Wuzhou Kale, Akshay Tzeng, Tzuen-Rong Wang, Yao-Nan Xuan, Xiangchun Micromachines (Basel) Article Focusing particles into a tight stream is critical for many microfluidic particle-handling devices such as flow cytometers and particle sorters. This work presents a fundamental study of the passive focusing of polystyrene particles in ratchet microchannels via direct current dielectrophoresis (DC DEP). We demonstrate using both experiments and simulation that particles achieve better focusing in a symmetric ratchet microchannel than in an asymmetric one, regardless of the particle movement direction in the latter. The particle focusing ratio, which is defined as the microchannel width over the particle stream width, is found to increase with an increase in particle size or electric field in the symmetric ratchet microchannel. Moreover, it exhibits an almost linear correlation with the number of ratchets, which can be explained by a theoretical formula that is obtained from a scaling analysis. In addition, we have demonstrated a DC dielectrophoretic focusing of yeast cells in the symmetric ratchet microchannel with minimal impact on the cell viability. MDPI 2020-04-25 /pmc/articles/PMC7281238/ /pubmed/32344887 http://dx.doi.org/10.3390/mi11050451 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lu, Song-Yu
Malekanfard, Amirreza
Beladi-Behbahani, Shayesteh
Zu, Wuzhou
Kale, Akshay
Tzeng, Tzuen-Rong
Wang, Yao-Nan
Xuan, Xiangchun
Passive Dielectrophoretic Focusing of Particles and Cells in Ratchet Microchannels
title Passive Dielectrophoretic Focusing of Particles and Cells in Ratchet Microchannels
title_full Passive Dielectrophoretic Focusing of Particles and Cells in Ratchet Microchannels
title_fullStr Passive Dielectrophoretic Focusing of Particles and Cells in Ratchet Microchannels
title_full_unstemmed Passive Dielectrophoretic Focusing of Particles and Cells in Ratchet Microchannels
title_short Passive Dielectrophoretic Focusing of Particles and Cells in Ratchet Microchannels
title_sort passive dielectrophoretic focusing of particles and cells in ratchet microchannels
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281238/
https://www.ncbi.nlm.nih.gov/pubmed/32344887
http://dx.doi.org/10.3390/mi11050451
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