Cargando…

Concentration Gradient Constructions Using Inertial Microfluidics for Studying Tumor Cell–Drug Interactions

With the continuous development of cancer therapy, conventional animal models have exposed a series of shortcomings such as ethical issues, being time consuming and having an expensive cost. As an alternative method, microfluidic devices have shown advantages in drug screening, which can effectively...

Descripción completa

Detalles Bibliográficos
Autores principales: Shen, Shaofei, Zhang, Fangjuan, Gao, Mengqi, Niu, Yanbing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281261/
https://www.ncbi.nlm.nih.gov/pubmed/32408585
http://dx.doi.org/10.3390/mi11050493
_version_ 1783543881879519232
author Shen, Shaofei
Zhang, Fangjuan
Gao, Mengqi
Niu, Yanbing
author_facet Shen, Shaofei
Zhang, Fangjuan
Gao, Mengqi
Niu, Yanbing
author_sort Shen, Shaofei
collection PubMed
description With the continuous development of cancer therapy, conventional animal models have exposed a series of shortcomings such as ethical issues, being time consuming and having an expensive cost. As an alternative method, microfluidic devices have shown advantages in drug screening, which can effectively shorten experimental time, reduce costs, improve efficiency, and achieve a large-scale, high-throughput and accurate analysis. However, most of these microfluidic technologies are established for narrow-range drug-concentration screening based on sensitive but limited flow rates. More simple, easy-to operate and wide-ranging concentration-gradient constructions for studying tumor cell–drug interactions in real-time have remained largely out of reach. Here, we proposed a simple and compact device that can quickly construct efficient and reliable drug-concentration gradients with a wide range of flow rates. The dynamic study of concentration-gradient formation based on successive spiral mixer regulations was investigated systematically and quantitatively. Accurate, stable, and controllable dual drug-concentration gradients were produced to evaluate simultaneously the efficacy of the anticancer drug against two tumor cell lines (human breast adenocarcinoma cells and human cervical carcinoma cells). Results showed that paclitaxel had dose-dependent effects on the two tumor cell lines under the same conditions, respectively. We expect this device to contribute to the development of microfluidic chips as a portable and economical product in terms of the potential of concentration gradient-related biochemical research.
format Online
Article
Text
id pubmed-7281261
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-72812612020-06-15 Concentration Gradient Constructions Using Inertial Microfluidics for Studying Tumor Cell–Drug Interactions Shen, Shaofei Zhang, Fangjuan Gao, Mengqi Niu, Yanbing Micromachines (Basel) Article With the continuous development of cancer therapy, conventional animal models have exposed a series of shortcomings such as ethical issues, being time consuming and having an expensive cost. As an alternative method, microfluidic devices have shown advantages in drug screening, which can effectively shorten experimental time, reduce costs, improve efficiency, and achieve a large-scale, high-throughput and accurate analysis. However, most of these microfluidic technologies are established for narrow-range drug-concentration screening based on sensitive but limited flow rates. More simple, easy-to operate and wide-ranging concentration-gradient constructions for studying tumor cell–drug interactions in real-time have remained largely out of reach. Here, we proposed a simple and compact device that can quickly construct efficient and reliable drug-concentration gradients with a wide range of flow rates. The dynamic study of concentration-gradient formation based on successive spiral mixer regulations was investigated systematically and quantitatively. Accurate, stable, and controllable dual drug-concentration gradients were produced to evaluate simultaneously the efficacy of the anticancer drug against two tumor cell lines (human breast adenocarcinoma cells and human cervical carcinoma cells). Results showed that paclitaxel had dose-dependent effects on the two tumor cell lines under the same conditions, respectively. We expect this device to contribute to the development of microfluidic chips as a portable and economical product in terms of the potential of concentration gradient-related biochemical research. MDPI 2020-05-12 /pmc/articles/PMC7281261/ /pubmed/32408585 http://dx.doi.org/10.3390/mi11050493 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shen, Shaofei
Zhang, Fangjuan
Gao, Mengqi
Niu, Yanbing
Concentration Gradient Constructions Using Inertial Microfluidics for Studying Tumor Cell–Drug Interactions
title Concentration Gradient Constructions Using Inertial Microfluidics for Studying Tumor Cell–Drug Interactions
title_full Concentration Gradient Constructions Using Inertial Microfluidics for Studying Tumor Cell–Drug Interactions
title_fullStr Concentration Gradient Constructions Using Inertial Microfluidics for Studying Tumor Cell–Drug Interactions
title_full_unstemmed Concentration Gradient Constructions Using Inertial Microfluidics for Studying Tumor Cell–Drug Interactions
title_short Concentration Gradient Constructions Using Inertial Microfluidics for Studying Tumor Cell–Drug Interactions
title_sort concentration gradient constructions using inertial microfluidics for studying tumor cell–drug interactions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281261/
https://www.ncbi.nlm.nih.gov/pubmed/32408585
http://dx.doi.org/10.3390/mi11050493
work_keys_str_mv AT shenshaofei concentrationgradientconstructionsusinginertialmicrofluidicsforstudyingtumorcelldruginteractions
AT zhangfangjuan concentrationgradientconstructionsusinginertialmicrofluidicsforstudyingtumorcelldruginteractions
AT gaomengqi concentrationgradientconstructionsusinginertialmicrofluidicsforstudyingtumorcelldruginteractions
AT niuyanbing concentrationgradientconstructionsusinginertialmicrofluidicsforstudyingtumorcelldruginteractions