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Concentration Gradient Constructions Using Inertial Microfluidics for Studying Tumor Cell–Drug Interactions
With the continuous development of cancer therapy, conventional animal models have exposed a series of shortcomings such as ethical issues, being time consuming and having an expensive cost. As an alternative method, microfluidic devices have shown advantages in drug screening, which can effectively...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281261/ https://www.ncbi.nlm.nih.gov/pubmed/32408585 http://dx.doi.org/10.3390/mi11050493 |
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author | Shen, Shaofei Zhang, Fangjuan Gao, Mengqi Niu, Yanbing |
author_facet | Shen, Shaofei Zhang, Fangjuan Gao, Mengqi Niu, Yanbing |
author_sort | Shen, Shaofei |
collection | PubMed |
description | With the continuous development of cancer therapy, conventional animal models have exposed a series of shortcomings such as ethical issues, being time consuming and having an expensive cost. As an alternative method, microfluidic devices have shown advantages in drug screening, which can effectively shorten experimental time, reduce costs, improve efficiency, and achieve a large-scale, high-throughput and accurate analysis. However, most of these microfluidic technologies are established for narrow-range drug-concentration screening based on sensitive but limited flow rates. More simple, easy-to operate and wide-ranging concentration-gradient constructions for studying tumor cell–drug interactions in real-time have remained largely out of reach. Here, we proposed a simple and compact device that can quickly construct efficient and reliable drug-concentration gradients with a wide range of flow rates. The dynamic study of concentration-gradient formation based on successive spiral mixer regulations was investigated systematically and quantitatively. Accurate, stable, and controllable dual drug-concentration gradients were produced to evaluate simultaneously the efficacy of the anticancer drug against two tumor cell lines (human breast adenocarcinoma cells and human cervical carcinoma cells). Results showed that paclitaxel had dose-dependent effects on the two tumor cell lines under the same conditions, respectively. We expect this device to contribute to the development of microfluidic chips as a portable and economical product in terms of the potential of concentration gradient-related biochemical research. |
format | Online Article Text |
id | pubmed-7281261 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72812612020-06-15 Concentration Gradient Constructions Using Inertial Microfluidics for Studying Tumor Cell–Drug Interactions Shen, Shaofei Zhang, Fangjuan Gao, Mengqi Niu, Yanbing Micromachines (Basel) Article With the continuous development of cancer therapy, conventional animal models have exposed a series of shortcomings such as ethical issues, being time consuming and having an expensive cost. As an alternative method, microfluidic devices have shown advantages in drug screening, which can effectively shorten experimental time, reduce costs, improve efficiency, and achieve a large-scale, high-throughput and accurate analysis. However, most of these microfluidic technologies are established for narrow-range drug-concentration screening based on sensitive but limited flow rates. More simple, easy-to operate and wide-ranging concentration-gradient constructions for studying tumor cell–drug interactions in real-time have remained largely out of reach. Here, we proposed a simple and compact device that can quickly construct efficient and reliable drug-concentration gradients with a wide range of flow rates. The dynamic study of concentration-gradient formation based on successive spiral mixer regulations was investigated systematically and quantitatively. Accurate, stable, and controllable dual drug-concentration gradients were produced to evaluate simultaneously the efficacy of the anticancer drug against two tumor cell lines (human breast adenocarcinoma cells and human cervical carcinoma cells). Results showed that paclitaxel had dose-dependent effects on the two tumor cell lines under the same conditions, respectively. We expect this device to contribute to the development of microfluidic chips as a portable and economical product in terms of the potential of concentration gradient-related biochemical research. MDPI 2020-05-12 /pmc/articles/PMC7281261/ /pubmed/32408585 http://dx.doi.org/10.3390/mi11050493 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Shen, Shaofei Zhang, Fangjuan Gao, Mengqi Niu, Yanbing Concentration Gradient Constructions Using Inertial Microfluidics for Studying Tumor Cell–Drug Interactions |
title | Concentration Gradient Constructions Using Inertial Microfluidics for Studying Tumor Cell–Drug Interactions |
title_full | Concentration Gradient Constructions Using Inertial Microfluidics for Studying Tumor Cell–Drug Interactions |
title_fullStr | Concentration Gradient Constructions Using Inertial Microfluidics for Studying Tumor Cell–Drug Interactions |
title_full_unstemmed | Concentration Gradient Constructions Using Inertial Microfluidics for Studying Tumor Cell–Drug Interactions |
title_short | Concentration Gradient Constructions Using Inertial Microfluidics for Studying Tumor Cell–Drug Interactions |
title_sort | concentration gradient constructions using inertial microfluidics for studying tumor cell–drug interactions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281261/ https://www.ncbi.nlm.nih.gov/pubmed/32408585 http://dx.doi.org/10.3390/mi11050493 |
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