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In vitro activity of Camellia sinensis (green tea) against trophozoites and cysts of Acanthamoeba castellanii

The effect of Camellia sinensis (green tea) on the growth of Acanthamoeba castellanii trophozoites was examined using a microplate based-Sulforhodamine B (SRB) assay. C. sinensis hot and cold brews at 75% and 100% concentrations significantly inhibited the growth of trophozoites. We also examined th...

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Autores principales: Fakae, Lenu B., Stevenson, Carl W., Zhu, Xing-Quan, Elsheikha, Hany M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281304/
https://www.ncbi.nlm.nih.gov/pubmed/32512260
http://dx.doi.org/10.1016/j.ijpddr.2020.05.001
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author Fakae, Lenu B.
Stevenson, Carl W.
Zhu, Xing-Quan
Elsheikha, Hany M.
author_facet Fakae, Lenu B.
Stevenson, Carl W.
Zhu, Xing-Quan
Elsheikha, Hany M.
author_sort Fakae, Lenu B.
collection PubMed
description The effect of Camellia sinensis (green tea) on the growth of Acanthamoeba castellanii trophozoites was examined using a microplate based-Sulforhodamine B (SRB) assay. C. sinensis hot and cold brews at 75% and 100% concentrations significantly inhibited the growth of trophozoites. We also examined the structural alterations in C. sinensis-treated trophozoites using transmission electron microscopy (TEM) and scanning electron microscopy (SEM). This analysis showed that C. sinensis compromised the cell membrane integrity and caused progressive destruction of trophozoites. C. sinensis also significantly inhibited the parasite's ability to form cysts in a dose-dependent manner and reduced the rate of excystation from cysts to trophozoites. C. sinensis exhibited low cytotoxic effects on primary corneal stromal cells. However, cytotoxicity was more pronounced in SV40-immortalized corneal epithelial cells. Chromatographic analysis showed that both hot and cold C. sinensis brews contained the same number and type of chemical compounds. This work demonstrated that C. sinensis has anti-acanthamoebic activity against trophozoite and cystic forms of A. castellanii. Further studies are warranted to identify the exact substances in C. sinensis that have the most potent anti-acanthamoebic effect.
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spelling pubmed-72813042020-06-10 In vitro activity of Camellia sinensis (green tea) against trophozoites and cysts of Acanthamoeba castellanii Fakae, Lenu B. Stevenson, Carl W. Zhu, Xing-Quan Elsheikha, Hany M. Int J Parasitol Drugs Drug Resist Article The effect of Camellia sinensis (green tea) on the growth of Acanthamoeba castellanii trophozoites was examined using a microplate based-Sulforhodamine B (SRB) assay. C. sinensis hot and cold brews at 75% and 100% concentrations significantly inhibited the growth of trophozoites. We also examined the structural alterations in C. sinensis-treated trophozoites using transmission electron microscopy (TEM) and scanning electron microscopy (SEM). This analysis showed that C. sinensis compromised the cell membrane integrity and caused progressive destruction of trophozoites. C. sinensis also significantly inhibited the parasite's ability to form cysts in a dose-dependent manner and reduced the rate of excystation from cysts to trophozoites. C. sinensis exhibited low cytotoxic effects on primary corneal stromal cells. However, cytotoxicity was more pronounced in SV40-immortalized corneal epithelial cells. Chromatographic analysis showed that both hot and cold C. sinensis brews contained the same number and type of chemical compounds. This work demonstrated that C. sinensis has anti-acanthamoebic activity against trophozoite and cystic forms of A. castellanii. Further studies are warranted to identify the exact substances in C. sinensis that have the most potent anti-acanthamoebic effect. Elsevier 2020-06-02 /pmc/articles/PMC7281304/ /pubmed/32512260 http://dx.doi.org/10.1016/j.ijpddr.2020.05.001 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fakae, Lenu B.
Stevenson, Carl W.
Zhu, Xing-Quan
Elsheikha, Hany M.
In vitro activity of Camellia sinensis (green tea) against trophozoites and cysts of Acanthamoeba castellanii
title In vitro activity of Camellia sinensis (green tea) against trophozoites and cysts of Acanthamoeba castellanii
title_full In vitro activity of Camellia sinensis (green tea) against trophozoites and cysts of Acanthamoeba castellanii
title_fullStr In vitro activity of Camellia sinensis (green tea) against trophozoites and cysts of Acanthamoeba castellanii
title_full_unstemmed In vitro activity of Camellia sinensis (green tea) against trophozoites and cysts of Acanthamoeba castellanii
title_short In vitro activity of Camellia sinensis (green tea) against trophozoites and cysts of Acanthamoeba castellanii
title_sort in vitro activity of camellia sinensis (green tea) against trophozoites and cysts of acanthamoeba castellanii
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281304/
https://www.ncbi.nlm.nih.gov/pubmed/32512260
http://dx.doi.org/10.1016/j.ijpddr.2020.05.001
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